Effect of administration timing of postchemotherapy granulocyte colony-stimulating factor on host-immune cell recovery and CD8<sup>+</sup> T-cell response

Salem, Mohamed L.; Nassef, Mohamed; Salam, Soha Gomaa Ramadan Abdel; Zidan, Abdel‐Aziz A.; Mahmoud, Mohamed H.; Badr, Gamal; Rubinstein, Mark P.; Cole, David J.

doi:10.1080/1547691x.2016.1194917

Cited by 7 publications

(10 citation statements)

References 20 publications

(29 reference statements)

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“…However, again, effects of the G-CSF were short-lived. In this study G-CSF showed immune stimulating effect resulted in ameliorating effect on leucocytes count in bone marrow and spleen, Also increasing the lymphocytes count, relative and absolute numbers of neutrophils These results were consistent with those from (Salem et al, 2016), (Demetri and Griffin 1991) who noted G-CSF administration induced increases in WBC counts by acting as a potent hematopoietic growth factor.…”

Section: Effect Of Sp Treatment On Alt and Ast Activitiessupporting

confidence: 92%

“…injection of PBS (Phosphate buffer saline) as control, once i.p. injection of CTX (4 mg/mouse) for induction of leucopenia, subcutaneous (s.c.) injection two days after CTX injection (the time point where leucopenia that occurred (Salem et al, 2016) of 5 µg/mouse G-CSF (Neupogen, CA, USA) daily for 5 consecutive days. To test the impact of SP on leucopenia, mice were treated for 7 consecutive days orally using stomach gavage of 500 mg/mouse starting on the day of CTX injection.…”

Section: Treatment Protocolmentioning

confidence: 99%

“…Despite its therapeutic benefits, CTX has been found to have several side effects as particular systemic leucopenia. Detailed analysis of this leucopenia in mice showed that CTX decreased the absolute number of leukocytes in the peripheral blood (PBL) at days 3-15, and in the spleen at days 3-6 and came back to normal range at the recovery phase (day 9) (Salem et al, 2009;Salem et al, 2010a and b;Salem et al, 2016;). These leucopenia effects of CTX make it a reliable model to study the ameliorating effects of certain compound on it.…”

Section: Introductionmentioning

confidence: 99%

“…Granulocyte colony-stimulating factor (G-CSF), a hematopoietic growth factor is considered as an adjuvant therapy during anti-cancer to correct leukopenia. It stimulates the immune response through activating the release of neutrophils from bone marrow to blood (Salem et al, 2016). G-CSF regulates granulocyte production and is produced by a diversity of cells in response to inflammation and infection (Gross-weege et al, 1997, Rubinstein et al, 2013.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Medhat

El‐Sheekh²,

Abo-Shady³

et al. 2019

Journal of Cancer and Biomedical Research

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Spirulina platensis (SP) is a blue-green alga used as a dietary supplement as it possesses antioxidant, anti-inflammatory and hepatoprotective effects with clinical importance in several disorders. The aim of this study was to evaluate the ameliorating effects of SP extract on toxicity induced mice by treatment with the anticancer drug cyclophosphamide (CTX). To this end, SP was cultured under optimal conditions of growth at pH 9 for 9 days using Zarrouk , s medium. The active ingredients including pigments, phycobiliproteins and total soluble proteins were estimated in SP suspensions which were then dried to a powder form. To assess antitoxicity, adult male albino mice were treated with interperitoneal (i.p.) injection of PBS, single dose of CTX (4mg/mouse), CTX (i.p.) followed by subcutaneous (s.c.) injection of G-CSF (5μg/mouse) daily for 5 consecutive days or CTX (i.p.) followed by oral administration of 0.5 gm/mouse SP extract for 7 consecutive days. After 7 days of initial treatment with CTX, mice were sacrificed; blood, spleen, bone marrow and liver were harvested to assess CBC, total count for spleen and bone marrow and liver for biochemical analysis. The results showed that SP showed the highest yield of dry weight and pigment content C-phycocyanin (CPC), allophycocyanin (APC), phycoerthrin (PE) and total phycobiliproteins (phycobilins) at the 9 th day of growth at pH 9 in culture. Oral administration of SP induced amelioration of CTX induced leucopenia in blood, bone marrow and spleen comparable to those of G-CSF. It also ameliorated the dysfunction in the liver enzymes ALT and AST. Interestingly, however, SP showed higher antioxidant effects than those of G-CSF as reflected by the higher activities of the anti-oxidant MDA and GSH. Conclusion: SP showed potent antitoxic effect through antioxidant activity and thus could be a useful co-adjuvant agent against chemotherapeutic drugs toxicity including cyclophosphamide. Protective effect of Spirulina platensis on Cyclophophamide …El-Sheekh et al., 2019

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Section: Effect Of Sp Treatment On Alt and Ast Activitiessupporting

confidence: 92%

Section: Treatment Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Medhat

El‐Sheekh²,

Abo-Shady³

et al. 2019

Journal of Cancer and Biomedical Research

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“…The findings from this study suggest that harnessing neutrophil cytotoxicity in bone by regulating neutrophil maturation and mobilization could prevent PCa growth in bone. For example, granulocyte colony-stimulating factor (G-CSF) is a primary regulator of neutrophil proliferation and differentiation and is often given to cancer patients to replenish the neutrophil population after chemotherapy-related neutropenia [43]. G-CSF mimetics are currently in clinical trial in combination with chemotherapy and could be considered for treatment of BM-PCa [44].…”

Section: Discussionmentioning

confidence: 99%

Neutrophils are mediators of metastatic prostate cancer progression in bone

Costanzo-Garvey

Keeley

Case

et al. 2020

Cancer Immunol Immunother

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Bone metastatic prostate cancer (BM-PCa) significantly reduces overall patient survival and is currently incurable. Current standard immunotherapy showed promising results for PCa patients with metastatic, but less advanced, disease (i.e., fewer than 20 bone lesions) suggesting that PCa growth in bone contributes to response to immunotherapy. We found that: (1) PCa stimulates recruitment of neutrophils, the most abundant immune cell in bone, and (2) that neutrophils heavily infiltrate regions of prostate tumor in bone of BM-PCa patients. Based on these findings, we examined the impact of direct neutrophilprostate cancer interactions on prostate cancer growth. Bone marrow neutrophils directly induced apoptosis of PCa in vitro and in vivo, such that neutrophil depletion in bone metastasis models enhanced BM-PCa growth. Neutrophil-mediated PCa killing was found to be mediated by suppression of STAT5, a transcription factor shown to promote PCa progression. However, as the tumor progressed in bone over time, neutrophils from late-stage bone tumors failed to elicit cytotoxic effector responses to PCa. These findings are the first to demonstrate that bone-resident neutrophils inhibit PCa and that BM-PCa are able to progress via evasion of neutrophil-mediated killing. Enhancing neutrophil cytotoxicity in bone may present a novel therapeutic option for bone metastatic prostate cancer.

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G‐CSF induces CD15⁺ CD14⁺ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment

et al. 2022

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Objectives. Recombinant granulocyte colony-stimulating factor (G-CSF) is frequently administered to patients with cancer to enhance granulocyte recovery post-chemotherapy. Clinical trials have also used G-CSF to modulate myeloid cell function in pregnancy and inflammatory diseases. Although the contribution of G-CSF to expanding normal granulocytes is well known, the effect of this cytokine on the phenotype and function of immunosuppressive granulocytic cells remains unclear. Here, we investigate the impact of physiological and iatrogenic G-CSF on an as yet undescribed granulocyte phenotype and ensuing outcome on T cells in the settings of cancer and pregnancy. Methods. Granulocytes from patients treated with recombinant G-CSF, patients with late-stage cancer and women enrolled on a trial of recombinant G-CSF were phenotyped by flow cytometry. The ability and mechanism of polarised granulocytes to suppress T-cell proliferation were assessed by cell proliferation assays, flow cytometry and ELISA. Results. We observed that G-CSF leads to a significant upregulation of CD14 expression on CD15 + granulocytes. These CD15 + CD14 + cells are identified in the blood of patients with patients undergoing neutrophil mobilisation with recombinant G-CSF, and physiologically in women early in pregnancy or in those treated as a part of a clinical trial. Immunohistochemistry of tumor tissue or placental tissue identified the expression of G-CSF. The G-CSF upregulates the release of reactive oxygen species (ROS) in CD15 + CD14 + cells leading to the suppression of T-cell proliferation. Conclusions. G-CSF induces a population of ROS + immunosuppressive CD15 + CD14 + granulocytes. Strategies for how recombinant G-CSF

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Effect of administration timing of postchemotherapy granulocyte colony-stimulating factor on host-immune cell recovery and CD8⁺ T-cell response

Cited by 7 publications

References 20 publications

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Neutrophils are mediators of metastatic prostate cancer progression in bone

G‐CSF induces CD15⁺ CD14⁺ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment

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Effect of administration timing of postchemotherapy granulocyte colony-stimulating factor on host-immune cell recovery and CD8+ T-cell response

Cited by 7 publications

References 20 publications

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Neutrophils are mediators of metastatic prostate cancer progression in bone

G‐CSF induces CD15+ CD14+ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment

Contact Info

Product

Resources

About

Effect of administration timing of postchemotherapy granulocyte colony-stimulating factor on host-immune cell recovery and CD8⁺ T-cell response

G‐CSF induces CD15⁺ CD14⁺ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment