Effect of adrenaline on glucose uptake by the canine larger bowel

Oyebola, D.D.O.

doi:10.4314/ajbr.v4i3.53891

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…[27] It is also reported that adrenaline produces hyperglycemia by increasing glucose uptake from both the large and small intestine. [28] The compound (CC2) probably prevented the rise in blood sugar by inhibiting adrenaline induced stimulation of α 2 receptors present in the pancreatic β-cells thus helping insulin release. [29,30] Estimation of glycogen level in animal studies may be considered as the best marker for assessing antihyperglycemic activity of any drug in experimental diabetes.…”

Section: Discussionmentioning

confidence: 99%

Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent

Osigwe¹,

Akah²,

Nworu³

et al. 2017

J Phytopharmacol

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The leaves of Newbouldia laevis is traditionally used to treat diabetes mellitus in southeast Nigeria. The apigenin isolated from the methanol fraction of dichloromethane/methanol (1:1) extract of the leaves was evaluated for antidiabetic and antihyperglycemic activity in alloxan-induced diabetic rats and on normal rats. Treatment of alloxan-diabetic rats with the compound (apigenin) significantly (p < 0.05) reduced blood glucose, and increased the liver and muscle glycogen content. The adrenaline-induced elevation of blood glucose of normal rats was significantly (p < 0.05) reduced by the isolated compound. These results suggest that apigenin may be the anti-diabetic principle in the leaves of Newbouldia laevis..

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Section: Discussionmentioning

confidence: 99%

Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent

Osigwe¹,

Akah²,

Nworu³

et al. 2017

J Phytopharmacol

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“…Adrenaline produces hyperglycemia by inhibiting insulin release, stimulating glycogenolysis in muscle and thus providing substrate in the form of lactate for hepatic gluconeogenesis, stimulating glucagon secretion and stimulating ACTH secretion which, in turn, stimulates glucocorticoid secretion from the adrenal cortex (Kraus-Friedmann, 1984; Krusteva, 1992; Alada et al, 2001). However, the test compounds did not significantly reduce the adrenaline induced hyperglycemia suggesting that they probably do not mediate their action via inhibition of adrenaline-induced stimulation of α 2 receptors in β-cells of pancreas and thus promoting hyperinsulinemia.…”

Section: Discussionmentioning

confidence: 99%

“…Group II rats served as the model control and were pretreated with 10 ml/kg per os 10% Tween 80-distilled water 1 hr before intraperitoneal injection of 50 µg/kg adrenaline. Groups III -VI rats which served as the treatment groups were orally pretreated with 50 mg/kg HU, HU Af , HU n and erinidine, respectively, for 1 hr before intraperitoneal injection of 50 µg/kg adrenaline, as described by Alada et al (2001).…”

Section: Adrenaline-induced Hyperglycemia Testmentioning

confidence: 99%

Antihyperglycemic profile of erinidine isolated from<i> Hunteria umbellate</i> seed

Adeneye

Fadhel-Albayati³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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Water decoction made from the seed of Hunteria umbellata is widely used in the traditional management of diabetes mellitus by Nigerian herbalists, particularly, in the southwest region of the country. Recently, a new bisindole alkaloid, erinidine, was isolated but its antihyperglycemic profile remains largely un-investigated scientifically. This forms the basis for the current study which is primarily designed at investigating the antihyperglycemic profile of erinidine and other fractions in both in vitro and in vivo models of diabetes mellitus. In the present study, erinidine was isolated and purified using the earlier described methods and its antihyperglycemic potentials tested in in vitro models such as dipeptidylpeptidase (IV), glycogen phosphorylase, HIT-T15 cell insulin secretion, glucose uptake activity, aldose reductase assays and α-glucosidase inhibition assay testings. In addition, 50 mg/kg of erinidine and that of other fractions were evaluated in in vivo models of normal and chemically-induced hyperglycemic rats. Results showed that erinidine was a light yellow, amorphous solid with UV (CHCl 3 ) λ max 256 nm, HRESIMS m/z 382.1881 [(M+H) + ] (calculated for C 22 H 26 N 4 O 2 , 382.1876) and melting point of 230 ºC. The in vitro study showed the antihyperglycemic action of erinidine to be weakly mediated via α-glucosidase inhibition mechanism as the results for other in vitro tests such as dipeptidylpeptidase (IV), glycogen phosphorylase, HIT-T15 cell insulin secretion, glucose uptake activity and aldose reductase assays were all negative. However, the in vivo results showed 50 mg/kg erinidine given per os to normal and alloxan-induced hyperglycemic rats to significantly (p<0.05, p<0.001) attenuate an increase in their post-absorptive blood glucose concentrations after 3 g/kg glucose loading in the rats, suggesting its antihyperglycemic mechanism to be via α-glucosidase inhibition. This result, although, further corroborated the in vitro findings but also suggests that erinidine needs to be biotransformed in vivo for its inhibitory activity on intestinal glucose absorption to become evident. Thus, the present study suggests erinidine to be the possible antihyperglycemic agent in Hunteria umbellata seed extract mediating its antihyperglycemic action via intestinal glucose uptake inhibition. Key words: Hunteria umbellata, Erinidine, Hyperglycemia, Alpha-glucosidase inhibitionList of Abbreviations ACTH -adrenocorticotropic hormone; ASW -automatic switchable; CHCl 3 -chloroform;12 CNMR -carbon nuclear magnetic resonance; DCM -dichloromethane; DM -diabetes mellitus; DMEM -Dulbecco's Modified Eagle medium; DMSOdimethylsulfoxide; DPBS -Dulbecco's phosphate buffer solution; DPP-IV -dipeptidylpeptidase-IV; ELISA -enzyme-linked immunosorbent assay; Gpa -glycogen phosphorylase; HBSS -Hank's balanced salt solution; HCl -hydrochloric acid; HIT-T15 -Insulin-secreting hamster insulinoma; HU Af -crude alkaloid fraction from the aqueous seed extract of Hunteria umbellata HU n -neutral compounds from the aque...

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“…Conversely, rats whose blood glucose levels surpassed 300 mg/dL were administered an injection of 20 IU/kg. A single subcutaneous injection of insulin was administered daily (12) . Concomitantly, 1 ml of saline was taken orally each day for duration of 4 weeks.…”

Section: -Diabetic +Insulin Groupmentioning

confidence: 99%

Effect of Glycemic Control Using Different Antidiabetic Drugs on Cognitive Functions in Experimentally-Induced Type 2DM: A Comparative Study

2024

The Egyptian Journal of Hospital Medicine

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Background: Neurocognitive impairment is recognized as the fourth microvascular complications of type 2 diabetes mellitus (T2DM). Objectives: To compare the effect of glycemic control on cognitive functions in diabetic rats by using different anti-diabetic drugs. Materials and Methods: Fifty male albino rats were equally categorized into: Control group, Janumet group, Diabetic non-treated group, Diabetic+Insulin group and Diabetic+Janumet group. At the end of 4 weeks, neurocognitive assessment was done by using Morris water maze (MWM) and Y-maze tests. Blood samples were collected to estimate glycemic state, lipid profile and total antioxidant capacity level (TAC). Rats' brains were extracted. The right half of the brain was utilized for lipid peroxidation marker (MDA), tumor necrosis factor alpha (TNF-α), superoxide dismutase (SOD), and interleukin 1B (IL-B) measurement. The left half was utilized for histopathological study of the hippocampal tissue. Results: Diabetic group showed significant increase in FBG, HBA1C, HOMA-IR, total cholesterol, triglyceride, MDA, TNF-alpha and IL-1B, and decrease in serum insulin, HDL, TAC and SOD. Neurocognitive impairment and hippocampal degenerative changes were also obvious in diabetic rats. Diabetic+ Janumet group demonstrated significant improvement in the measured biomarkers and neurobehavioral tests with restoration of the hippocampal tissue structure in comparison with Diabetic+ Insulin group. However, there was an insignificant difference in lipid profile markers between Diabetic+ Janumet group and Diabetic+ Insulin group. Insignificant difference between Control and Janumet groups regarding all measured parameters was detected. Conclusion: Both insulin and Janumet drugs alleviates the metabolic, neurocognitive impairment and hippocampal changes associated with T2DM. However, the improvement was more pronounced with Janumet treatment than insulin treatment. The synergistic hypoglycemic, anti-oxidant and anti-inflammatory effects of Janumet's components may account for this improvement.

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Effect of adrenaline on glucose uptake by the canine larger bowel

Cited by 6 publications

References 8 publications

Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent

Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent

Antihyperglycemic profile of erinidine isolated from<i> Hunteria umbellate</i> seed

Effect of Glycemic Control Using Different Antidiabetic Drugs on Cognitive Functions in Experimentally-Induced Type 2DM: A Comparative Study

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