Effect of Adrenotropic Hormone on Ascorbic Acid and Cholesterol Content of the Adrenal.

Chemical Changes in the Tissues of the Rat After Adrenalectomy

“…This procedure was found to be associated with a marked fall in the ascorbic acid content of the adrenal gland. Since such a fall is a feature of the adrenal response to stress [Sayers, Sayers, Lewis & Long, 1944;Sayers, Sayers, Liang & Long, 1945] the use of sucrose and inulin was abandoned. In the experiments recorded below ammonium thiocyanate was used to estimate the extracellular fluid volume, since it can be determined accurately in low concentrations and administered in small amounts.…”

60 YEARS OF POMC: N-terminal POMC peptides and adrenal growth

“…However, the adrenal weight assay is incredibly insensitive requiring large amounts of pituitary extract to see an effect, and inevitably, this led to a quest to find a better bioassay. The first alternative was an assay developed by Sayers, which was based on the observation that levels of ascorbic acid are depleted in adrenals following treatment with pituitary extracts (Sayers et al 1944). Unknown at the time, it is actually ACTH that causes this effect (the significance of the depletion is still unclear), and with the further development in the 1950s of assays using corticosteroids as the endpoint, the scene was set to identify what we now call ACTH (Bell 1954, Howard et al 1955, Shepherd et al 1956).…”

Changes in Rat Adrenal Ascorbic Acid and Glutathione Following Hypophysectomy and ACTH Stimulation1

“…A paralReceived January 28,1963. 1 Presented in part to the 43rd Meeting of the Endocrine Society, New York, 1961. 2 Supported in part by American Cancer Society Fellowship PF-89, USPH Research Grant #A-4131 and USPH Training Grant #2A-5176.…”