Effect of Adriamycin on the Reproductive Integrity of Cultured Leukemia L1210 and P388 Cells 2

Abstract

Proliferating cultured P388 cells exhibited a greater degree of sensitivity to adriamycin than did proliferating cultured L1210 cells, although both leukemia cell populations had approximately the same doubling time. The rate of reduction in viability when cultured L1210 cell populations were exposed to adriamycin (0.0625-2.0 microgram/ml, concentrations that are comparable to tissue drug levels during therapy) was concentration-dependent. Therefore, the results indicated a possible therapeutic advantage to be gained by an increase in drug concentrations (within the limits of acceptable host toxicity) at the target cell site.

Collateral Sensitivity to Methotrexate and Dichloromethotrexate of Cultured Lymphocytic Leukemia L1210 Cells Resistant to 6-Mercaptopurine

“…The L1210/ 6-MP lymphocytic leukemia developed by Law et al (3) and the L1210/0 lymphocytic leukemia were obtained from the tumor bank of the Division of Drug Cancer Treatment, National Cancer Institute, Bethesda, Maryland. Cultured cell populations derived from L1210/0 and L1210/6-MP lymphocytic leukemias transplanted in DBA/2 mice were propagated in Dulbecco's modified eagle's minimum essential medium (DMMEM) supplemented with 10% horse serum with 5 p M 2-mercaptoethanol (growth medium) (5). The cell lines were maintained in stationary suspension cultures.…”

“…Proliferating cell populations were exposed to either 6-MP (1.7-170 pglml) for 24 o r 48 hr, or to MTX (0.055-5.5 pg/ml), o r DCMTX (1.0-100 pg/ml) for 24 hr. The cells were harvested by sedimentation, washed in a balanced salts solution, and the number of cells surviving drug treatment was determined by colony formation in semisolid medium (5).…”