Effect of aeration on the fertilising ability of turkey semen stored for 48 hours at 5 and 15 °C : A study from the 33rd to the 47th week of age

Abstract

Introduction.

Current role of semen storage and artificial insemination in the turkey industry

“…Constant or intermittent agitation (5 min/h) with a circular shaker at 150 to 300 rpm, or aeration using a peristaltic pump where a Teflon tube brings the air inside the sample, are very effective (Huyghebaert et al, 1985;Lake et al, 1984). The basic principle is that each spermatozoon is supplied regularly with oxygen, and one of the first conditions to meet this requirement is that the air is brought to the bottom of the sample (Huyghebaert et al, 1984).…”

“…However, differences in fertility were observed between diluents when semen was stored at 5 or 10°C (Huyghebaert and De Groóte, 1983). Lake et al (1984) compared temperatures of 5 and 15°C for 24 and 48 h storage, respectively. Although the loss in fertility after 24 h of storage was only 20% when semen was stored at 15°C, the difference was increased to 40% after 48 h of storage.…”

“…In a recent study, Sexton (1988) demonstrated major differences in fertility between diluents after 24 h of storage at 5°C. Lake et al (1984) modified their original diluent (Lake and Ravie, 1982) according to the composition of the turkey ductus deferens fluid. However, a direct comparison between the two storage media was not made.…”

“…Lake et al (1984) stored semen for 48 h and agitated the samples constantly. With weekly inseminations of maximum 180 X 10 6 spermatozoa (after the 6th week), 85-6% fertility was obtained during a 14-week period, while the control treatment produced 93-8% fertility.…”

Parâmetros reprodutivos de perdizes machos (Rhynchotus rufescens) criadas em cativeiro: comparação entre os índices reprodutivos de animais acasalados e inseminados

“…A composição iônica do diluente geralmente é baseada na composição do plasma seminal (LAKE; CHERMS; WISHART, 1984). Porém, dependendo da temperatura de estoque, a concentração de íons ou de cátions pode variar (CHAUDHURI; LAKE, 1988) e meios completamente diferentes podem ser usados com os mesmos resultados.…”