Effect of aflatoxin B1 on blood serum oestradiol-17² and progesterone concentrations during the luteal phase and the synchronized oestrus of goats

Abstract

The effect of prolonged aflatoxin B 1 (AFB 1 ) administration on blood serum oestradiol-17β and progesterone concentrations in goats during the luteal phase and the synchronized oestrus was investigated. Thirty-six Greek indigenous primiparous goats were used, during the oestrus period; 12 goats received, per os, 50 μg (treated group T50) and 12 goats received 100 μg (treated group T100) AFB 1 /day/head, respectively, for approximately 1.5 month, while 12 goats served as controls (C). On day 36 of the experiment, each goat was injected, i.m, 0.5 ml prostaglandin F 2α (PGF 2α ). Blood samples were collected from each goat twice a week, before PGF 2α injection, as well as every 4 hours from the onset to the end of the synchronized oestrus. Oestradiol-17β and progesterone concentrations in blood serum were determined using radioimmunoassay. During the whole luteal(s) phase(s), linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β and a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100), in a dose dependent manner. During the synchronized oestrus, multiple linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β, as well as a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100) and over time (hours, from the onset to the end of the synchronized oestrus). No significant differences were noticed among the three groups, regarding the body weight of the goats from the onset to the end of AFB 1 administration, the occurrence or the duration of the synchronized oestrus presented by the goats (P > 0.05).In conclusion, prolonged AFB 1 administration at doses of 100 or even of 50 μg/day/head changes the hormonal pattern in blood during the luteal phase and the synchronized oestrus of goats, being in oestrus period.

Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1

“…AFBO can also combine with DNA to form AFB1-N7-Gua, causing DNA mutation [9,10]. AFB1 reduces steroid production by competitively binding StAR protein of rats, affects the secretion of estradiol-17β and progesterone in animal serum, inhibits the growth of oocytes and leads to the decrease of ovarian size and weight [11,12]. In male mice, AFB1 exposure is related to histological changes of testis, reduction of sperm number and differences in sperm motility and litter size [13,14].…”

In Vivo Follicular and Uterine Arterial Indices as an Indicator of Successful Hormonal Stimulation for Inactive Ovaries in Repeat-Breeder Crossbred Dairy Cows Using a Short-Term Progesterone-Based Programme

“…However, the investigation of vascularity of ovarian and uterine arteries after the hormonal treatment for inactive ovaries using the short-term P 4 -based programme has not yet been investigated in repeat-breeder crossbred dairy cows. To the best of the researchers' knowledge, a greater knowledge of uterine and ovarian biologies is essential to increase the applied reproduction in female ruminant animals [8,14].…”

A systems biology approach reveals the endocrine disrupting potential of Aflatoxin B1.

“…Bioinformatics analysis of gene expression patterns deregulated by AFB1 and AFM1 indicated perturbation of biological pathways inherent to steroid hormone synthesis, nuclear receptor and estrogen signaling pathways suggesting an inherent estrogenic component in the mechanism of action of aflatoxin (Marchese et al 2018). AFB1 and its metabolite AFL, produced by an undefined NADPH reductase, can cross the placenta and be reconverted to AFB1 (Storvik et al 2011;Huuskonen et al 2013 Studies in AFB1-exposed goats revealed altered levels of estradiol and progesterone in circulating blood, suggesting effective hormonal disruption by the mycotoxin (Kourousekos and Theodosiadou 2015;Kourousekos et al 2018). Additionally, the selective estrogen receptor modulator Tamoxifen prevented AFB1 from interacting with enzymes involved in placenta hormone production (CYP19A1) and decreased accumulation of its metabolite Aflatoxicol (Storvik et al 2011).…”