Effect of aflatoxin B1 on the seminiferous tubules and the possible protective role of curcumin in adult albino rats (Light and electron microscopic study)

Elkordy, Eman Ali; Gazia, Maha Mohamed Abo

doi:10.1097/01.ehx.0000481141.52450.03

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Any structural changes including histomorphometric and spermiogenic alterations could be related to the toxic agent‐induced direct and/or indirect actions. We and others previously showed that not only CPA exposure but other mycotoxins including aflatoxin B1, ochratoxin A, fumonesin B1, and zearalenone exposure resulted in direct structural changes such as vacuolation in spermatogonia, creation of multinuclear giant cells, disorganization, changes in the number and thickness of spermatogenesis cell series, atrophy and necrosis of the seminiferous tubules, thickening in the basement membrane of the testis and epithelium of the seminiferous tubules 50‐55 . The direct effects of toxicants may related to their chemical reactivity with target site and/or any changes in the function of various cells and organelles.…”

Section: Discussionmentioning

confidence: 95%

Cyclopiazonic acid induced p53‐dependent apoptosis in the testis of mice: Another male related risk factor of infertility

Bonyadi

Hasanzadeh

Malekinejad

2021

Environmental Toxicology

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Cyclopiazonic acid (CPA) is an indole tetrameric acid mycotoxin. This study carried out to investigate the potential effects of CPA on male reproductive system. In the current study, 40 adult male mice were divided into five groups (n = 8). The control group did not expose to CPA, while animals in vehicle‐received group; received the CPA‐solvent (0.05% dimethyl sulfoxide) and the animals of third, fourth, and fifth groups received CPA 0.03, 0.06, and 0.12 mg/kg, body weight, respectively for 28 days. Morphometric and morphological deviations, spermatogenesis indices, malondialdehyde (MDA) content, total thiol molecules (TTM) concentration, total antioxidant capacity (TAC), protein carbonylation rate (CO), and nitric oxide (NO) concentration were examined. The expression changes of apoptotic genes (P53, Bcl‐2, and Caspase III) at mRNA level were also evaluated by qPCR technique. Reduction in the Leydig and Sertoli cells population, diameter of seminiferous tubules, and spermatogenesis parameters was significant only in the group that received the highest dose of CPA. An increase in the level of MDA, NO, and CO in testicular tissue and reduction of TAC and TTM were observed in the CPA‐exposed groups. Significant up‐regulation (p < .05) in the expression of P53 and Caspase III genes and down‐regulation of Bcl‐2 gene were found in the CPA‐received groups. These results are indicating the detrimental effects of CPA on the testicles, which may attribute to the CPA‐induced oxidative stress and apoptosis. Moreover, results also help to understand a serious concern about the presence of CPA in foods as a potential risk factor in male‐related infertility.

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Section: Discussionmentioning

confidence: 95%

Cyclopiazonic acid induced p53‐dependent apoptosis in the testis of mice: Another male related risk factor of infertility

Bonyadi

Hasanzadeh

Malekinejad

2021

Environmental Toxicology

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“…With disorganized germinal epithelium and thickened basement membranes, some tubules shrank. The majority of the spermatogenic and Sertoli cells had pyknotic nuclei and vacuolated cytoplasm [ 57 ]. Aboelhassan et al [ 58 ] revealed that, when compared to the control group, the AFB1 therapy caused some seminiferous tubules to degenerate in the testis.…”

Section: Discussionmentioning

confidence: 99%

Camel milk or silymarin could improve the negative effects that experimentally produced by aflatoxin B1 on rat’s male reproductive system

Hassaneen,

Hemeda,

El Nahas

et al. 2024

BMC Vet Res

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Background Camel milk and silymarin have many different beneficial effects on several animal species. Meanwhile, Aflatoxins are mycotoxins with extraordinary potency that pose major health risks to several animal species. Additionally, it has been documented that aflatoxins harm the reproductive systems of a variety of domestic animals. The present design aimed to investigate the impact of aflatoxin B1 (AFB1) on rat body weight and reproductive organs and the ameliorative effects of camel milk and silymarin through measured serum testosterone, testes pathology, and gene expression of tumor necrosis factor (TNF-α), luteinizing hormone receptor (LHR), and steroidogenic acute regulatory protein (StAR) in the testes. A total of sixty mature male Wister white rats, each weighing an average of 83.67 ± 0.21 g, were used. There were six groups created from the rats. Each division had ten rats. The groups were the control (without any treatment), CM (1 ml of camel milk/kg body weight orally), S (20 mg silymarin/kg b. wt. suspension, orally), A (1.4 mg aflatoxin/kg diet), ACM (aflatoxin plus camel milk), and AS (aflatoxin plus silymarin). Results The results indicated the positive effects of camel milk and silymarin on growth, reproductive organs, and gene expression of TNF-α, LHR, and StAR with normal testicular architecture. Also, the negative effect of AFB1 on the rat’s body weight and reproductive organs, as indicated by low body weight and testosterone concentration, was confirmed by the results of histopathology and gene expression. However, these negative effects were ameliorated by the ingestion of camel milk and silymarin. Conclusion In conclusion, camel milk and silymarin could mitigate the negative effect of AFB1 on rat body weight and reproductive organs.

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Lycopene ameliorates aflatoxin B1-induced testicular lesion by attenuating oxidative stress and mitochondrial damage with Nrf2 activation in mice

Huang

Cao

Cui

et al. 2023

Ecotoxicology and Environmental Safety

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Effect of aflatoxin B1 on the seminiferous tubules and the possible protective role of curcumin in adult albino rats (Light and electron microscopic study)

Cited by 4 publications

References 46 publications

Cyclopiazonic acid induced p53‐dependent apoptosis in the testis of mice: Another male related risk factor of infertility

Cyclopiazonic acid induced p53‐dependent apoptosis in the testis of mice: Another male related risk factor of infertility

Camel milk or silymarin could improve the negative effects that experimentally produced by aflatoxin B1 on rat’s male reproductive system

Lycopene ameliorates aflatoxin B1-induced testicular lesion by attenuating oxidative stress and mitochondrial damage with Nrf2 activation in mice

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