Effect of Age, Nutrition, Sex, and Time of Day on Response of Boll Weevils to an Attractant from Cotton12

Hardee, D. D.; Mitchell, E. B.; Huddleston, P.M.

doi:10.1093/aesa/59.5.1024

Cited by 12 publications

(7 citation statements)

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“…Each sex was isolated in a separate rearing chamber at 13 h photophase, 25 ± 4 °C and 60 ± 10% RH until the bioassay. All food sources were removed on the third day, and beetles were maintained on water until they were used in the experiments when they were five days old, at which time they would be maximally responsive to semiochemicals 44 , 45 . At least 2 h before the experiment, beetles were isolated by sex and placed individually into 1.5 mL microtubes containing a small hole in the bottom and top to allow air flux during the semiochemical experiments and isolated by sex until the experiment.…”

Section: Methodsmentioning

confidence: 99%

Systemic and sex-biased regulation of OBP expression under semiochemical stimuli

Paula

Togawa

Costa

et al. 2018

Sci Rep

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Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement in insect olfaction, while their regulation in response to semiochemical stimuli has remained poorly known. Previous studies of semiochemical response were basically done using electrophysiology, which integrates the response of the set of OBPs present in an antenna or sensillum, without revealing the regulation of OBPs or which ones might be involved. In this study we used boll weevil as a model and mined its OBPs by RNA-Seq to study their simultaneous antennal expression by qPCR under controlled semiochemical stimuli with aggregation pheromone and plant volatiles. In the absence of a semiochemical stimulus, 23 of 24 OBPs were constitutively expressed in the antenna in both sexes. Semiochemicals changed systemically the expression of OBPs in both sexes. There were different patterns of up- and down-regulation in female antennae for each semiochemical stimulus, consistent with female chemical ecology. On the other hand, the only response in males was down-regulation of some OBPs. We suggest that these systemic changes in OBP expression might be related to enhancing detection of the semiochemical stimuli and/or priming the olfactory system to detect other environmental chemicals.

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Section: Methodsmentioning

confidence: 99%

Systemic and sex-biased regulation of OBP expression under semiochemical stimuli

Paula

Togawa

Costa

et al. 2018

Sci Rep

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“…Many endogenous factors, including age, physiological state, nutrition, and sex of the individual can affect behavioral responses (e.g., Hardee et al 1966;Hawkes & Coaker 1976). Exogenous factors including level of illumination (Sparks & Cheatham 1970), humidity (e.g., Traynier 1967), time of day (Hardee et al 1966), and even barometric pressure (Steinberg et al 1992) also may influence responses. Ultimately, the careful use of extensive controls, both positive and negative, is the best approach to identify and account for effects of such variables.…”

Section: General Considerations For Laboratory Bioassaysmentioning

confidence: 99%

Bioassay Methods with Terrestrial Invertebrates

Hare¹

1998

Methods in Chemical Ecology Volume 2

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“…The active crude extract was tested in an olfactometer developed by Hardee et al, (1966). Hedin et al, (Pets.…”

Section: Field Plot Studies With Frego Bract Cottonsmentioning

confidence: 99%

FACTORS CONTRIBUTING TO RESISTANCE AND SUSCEPTIBILITY OF COTTON AND OTHER HOSTS TO THE BOLL WEEVIL, ANTHONOMUS GRANDIS

Maxwell

Jenkins

Parrott

et al. 1969

Entomologia Exp Applicata

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A large portion of the world's germ plasm of cotton has been screened at the USDA Boll Weevil Research Laboratory, State College, Mississippi during the period 1962--68 utilizing developed techniques.An oviposition suppression factor causing 25---40.% reduction in number of eggs laid by the weevil has been found in Gossypium barbadense and successfully moved into upland cotton, G. hirsutum.Two years field data with frego cottons indicate that the frego character contributes a significant degree of non-preference for egg laying by the boll weevil under field conditions. The frego character is currently being combined with selected lines of G. hirsutum carrying the oviposition suppression factor.A seedling screening technique utilizing field cages and greenhouse sand flats has been shown to have significant value in screening for individual resistant plants within a commercial variety. A number of selections with 25 % or more resistance to oviposition are under extensive investigation.A number of biologically active materials have been detected in the cotton plant and other hosts of the boll weevil which significantly influences feeding stimulation, feeding deterrency, repellency and attractancy of the weevil to its hosts.A highly active square abscission factor has been detected and extracted successfully from 2nd and 3rd instar larvae. The material injected into cotton squares causes 95% abscission of squares within 48--60 hours.The boll weevil, Anthonomus grandis Boheman, is a very destructive insect on cotton, Gossypium hirsutum L. in the U.S. Cotton Belt. The weevil first entered the country from Mexico in 1892 and by 1922 had covered the entire Cotton Belt. Knipling (1966) estimated that the boll weevil causes a $300 million annual loss plus insecticide costs estimated at $70 million annually.The USDA Boll Weevil Research Laboratory was built and staffed with personnel dedicated to developing "new approaches" which would lead to the economic control and hopefully eventual eradication of this pest without increasing the use of insecticides in our environment.Host plant resistance was one of the areas selected for major emphasis at this laboratory. The host plant resistance team, composed of geneticists and entomologists, began accumulating and screening the world germ plasm of cotton in 1962.

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Effect of Age, Nutrition, Sex, and Time of Day on Response of Boll Weevils to an Attractant from Cotton12

Cited by 12 publications

References 0 publications

Systemic and sex-biased regulation of OBP expression under semiochemical stimuli

Systemic and sex-biased regulation of OBP expression under semiochemical stimuli

Bioassay Methods with Terrestrial Invertebrates

FACTORS CONTRIBUTING TO RESISTANCE AND SUSCEPTIBILITY OF COTTON AND OTHER HOSTS TO THE BOLL WEEVIL, ANTHONOMUS GRANDIS

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