Effect of age on cerebrospinal fluid levels of metabolites of biopterin and biogenic amines

Komori, H.; Matsuishi, T.; Yamada, S.; Ueda, N.; Yamashita, Y.; Kato, H.

doi:10.1080/080352599750030059

Cited by 4 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding has been explained by the presence of large amounts of the key enzymes regulating both metabolic pathways during the mitosis, neurogenesis, migration and network formation of dopamine and serotonin neurons in foetus brain (Sladek et al, 1995). In agreement with previous studies (Hyland et al, 1993;Komori et al, 1999), we also observed a negative association between all BA and the age of controls. This finding led us to stratify reference values into six groups according to age.…”

Section: Discussionsupporting

confidence: 94%

“…This finding led us to stratify reference values into six groups according to age. We observed significantly higher 5-HIAA and HVA values in controls younger than 1 month compared with controls of 2-5 months (Table 2), in contrast to previously reported reference values (Hyland et al, 1993;Komori et al, 1999), which included larger ageintervals for the reference groups. It is likely that the different number of controls studied and the age-distribution of them may explain these differences.…”

Section: Discussioncontrasting

confidence: 86%

“…Moreover, the number of subjects studied for reference values establishment was limited (75 in reference from Hyland et al, 1993; 56 in reference from Komori et al, 1999). Higher 5-HIAA and HVA CSF concentrations have been observed in the first months of life.…”

Section: Discussionmentioning

confidence: 95%

“…However, there are few reports concerning detailed analytical procedures for the diagnosis of these congenital disorders. Moreover, data concerning reference values in a paediatric population have scarcely been reported (Hyland et al, 1993;Komori et al, 1999), to our knowledge.…”

Section: Introductionmentioning

confidence: 91%

See 3 more Smart Citations

HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins

Ormazábal

García‐Cazorla

Fernández

et al. 2005

Journal of Neuroscience Methods

107

112

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 86%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 91%

See 2 more Smart Citations

HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins

Ormazábal

García‐Cazorla

Fernández

et al. 2005

Journal of Neuroscience Methods

107

112

View full text Add to dashboard Cite

“…As oxidized pterins are fluorescent while their reduced counterparts are not, the most widely used method for pterin determination in CSF requires carrying out two HPLC (high performance liquid chromatography) runs coupled to fluorescence detection (FD) including two off-line iodine oxidation procedures for complete analysis. − The first run involves oxidation under acidic conditions of both BH4 and BH2 into biopterin (B), and NH2 into neopterin (N) prior to injection into the chromatograph. The second run involves the oxidation under alkaline conditions of BH2 into B and BH4 into pterin (P).…”

mentioning

confidence: 99%

Simultaneous Determination of All Forms of Biopterin and Neopterin in Cerebrospinal Fluid

Guibal

Lévêque

Doummar

et al. 2014

ACS Chem. Neurosci.

View full text Add to dashboard Cite

In humans, genetic defects of the synthesis or regeneration of tetrahydrobiopterin (BH4), an essential cofactor in hydroxylation reactions, are associated with severe neurological disorders. The diagnosis of these conditions relies on the determination of BH4, dihydrobiopterin (BH2), and dihydroneopterin (NH2) in cerebrospinal fluid (CSF). As MS/MS is less sensitive than fluorescence detection (FD) for this purpose, the most widely used method since 1980 involves two HPLC runs including two differential off-line chemical oxidation procedures aiming to transform the reduced pterins into their fully oxidized fluorescent counterparts, biopterin (B) and neopterin (N). However, this tedious and time-consuming two-step indirect method underestimates BH4, BH2, and NH2 concentrations. Direct quantification of BH4 is essential for studying its metabolism and for monitoring the efficacy of BH4 supplementation in patients with genetic defects. Here we describe a single step method to simultaneously measure BH4, BH2, B, NH2, and N in CSF by HPLC coupled to FD after postcolumn coulometric oxidation. All target pterins were quantified in CSF with a small volume (100 μL), and a single filtration step for sample preparation and analysis. As compared to the most widely used method in more than 100 CSF samples, this new assay is the easiest route for accurately determining in a single run BH4, BH2, and NH2 in CSF in deficit situations as well as for monitoring the efficacy of the treatment.

show abstract

Early life stress and novelty seeking behavior in adolescent monkeys

Parker

Rainwater

Buckmaster

et al. 2007

Psychoneuroendocrinology

View full text Add to dashboard Cite

SUMMARYRecent evidence suggests that early exposure to mild stress promotes the development of novelty seeking behavior. Here we test this hypothesis in squirrel monkeys and investigate whether novelty seeking behavior is associated with differences in cerebrospinal fluid (CSF) levels of the serotonin metabolite 5-hydroxyindoleacetic acid (5HIAA), the dopamine metabolite homovanillic acid (HVA), the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethylene glycol (MHPG), and the neuropeptide corticotrophin-releasing factor (CRF). Monkeys were randomized early in life to either mild intermittent stress (IS) or no stress (NS) conditions, and subsequently presented with opportunities to interact with a familiar or novel object in a test box that was connected to each monkey's home cage. To further minimize the potentially stressful nature of the test situation, monkeys were acclimated to the test procedures prior to study initiation. Post-test plasma levels of cortisol in IS and NS monkeys did not differ significantly from baseline levels measured in undisturbed conditions. During testing, more IS than NS monkeys voluntarily left the home cage, and IS monkeys spent more time in the test box compared to NS monkeys. More IS than NS monkeys engaged in object exploration in the test box, and IS monkeys preferred to interact with the novel vs. familiar object. Novelty seeking was not associated with differences in 5HIAA, HVA, MHPG, or CRF, but correlated with differences in object exploration observed in a different test situation at an earlier age. These trait-like differences in novelty seeking appear to reflect mild early stress-induced adaptations that enhance curiosity and resilience.

show abstract

Effect of age on cerebrospinal fluid levels of metabolites of biopterin and biogenic amines

Cited by 4 publications

References 12 publications

HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins

HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins

Simultaneous Determination of All Forms of Biopterin and Neopterin in Cerebrospinal Fluid

Early life stress and novelty seeking behavior in adolescent monkeys

Contact Info

Product

Resources

About