Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Ahmed, S. I.; Khan, M. Islam; Ahmad, Mushtaq; Iqbal, Sajid

doi:10.1080/1828051x.2018.1424569

Cited by 31 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At 7 years old, swamp buffalo bull had the poorest (P<0.01) sperms motility postthawing and recovery rate compared to 5 and 6 years old. In line with this finding, Ahmed et al (2018) also found that aged buffalo bulls had lower sperms motility post-thawing than the young bulls. In other livestock such as dairy cattle, it was also reported that the bull age had a significant effect on sperms motility post-thawing (Murphy et al, 2018).…”

Section: Resultssupporting

confidence: 77%

See 1 more Smart Citation

The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center

Isnaini

Harsi²,

Zamani

2019

JIIP

View full text Add to dashboard Cite

This study aimed to assess the effect of age on semen freezability (sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate) of swamp buffalo bull kept in Lembang Artificial Insemination Center. A total of 115 ejaculates collected from one swamp buffalo bull at 5, 6 and 7 years old were included in the study. The results showed that the overall mean values of sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate of swamp buffalo bull were 62.13%, 43.61%, 62.16%, and 114.16 doses, respectively. The age of bull did not show significant (P>0.05) effect on sperms motility before freezing, however the sperms motility post-thawing, recovery rate, and straws production per ejaculate was influenced (P<0.01) by bull age. At seven years old, the poorest (P<0.01) post-thawing sperms motility and recovery rate were noted in comparison to 5 and 6 years old, nevertheless the difference in post-thawing sperms motility was only less than 3% point, which was not biologically important. On the other hand, the semen collection at seven years old had a higher (P<0.01) straws production per ejaculate than those at 5 and 6 years old (133.70 vs. 106.39 and 90.61 doses, respectively). In conclusion, this study provides evidence that the semen freezability of swamp buffalo bull is varied among the age with the peak frozen semen production was achieved at seven years old.

show abstract

Section: Resultssupporting

confidence: 77%

“…Previously, Amin et al (1999) showed that sperms motility post-thawing of swamp buffalo bulls ranged from 41.43 to 55.71%. In another study, Ahmed et al (2018) found that sperms motility post-thawing of Nili-Ravi buffalo bulls was between 43.80 to 46.60%.…”

Section: Resultsmentioning

confidence: 95%

The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center

Isnaini

Harsi²,

Zamani

2019

JIIP

View full text Add to dashboard Cite

show abstract

“…Sperm abnormality can be categorized as primary or secondary abnormality both have different causes. Primary abnormality ccurred on the spermatogenesis process inside testis because the maturation process of sperm did not function properly, while secondary abnormality often occurred after sperm cells leaving tubulous seminiferous or outside main reproductive organs [21]. Sperm abnormality on frozen semen generally classified as a secondary abnormality which was caused by few defections on several parts of the sperm cell body especially on sperm tail.…”

Section: Sperm Abnormalitymentioning

confidence: 99%

Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality

2020

View full text Add to dashboard Cite

The purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment in addition to the extender consisted of T0 (no addition of vitamin E), T1 (0,134 gram/100 ml extender), T2 (0,268 gram/100 ml extender) and T3 (0,402 gram/100 ml extender). Post-thawing evaluation conducted 24 hours after the freezing process and observed after the thawing process in 37°C water bath for 30 seconds. Parameters observed in this research were the post-thawing quality of frozen-thawed Simmental bull semen based on motility, mortality and abnormality percentage. Sperm motility evaluated using a microscope with 100x and 400x magnifying, sperm mortality observed using 400x magnifying and counted based on 0,2% eosin-negrosin staining, sperm abnormality observed using 400x magnifying and counted based on the number of morphologically abnormal sperm cells. Semen post-thawing motility was not significantly affected by vitamin E addition of T1, T2 and T3 (P<0,05). T1 and T2 were able to significantly lower the mortality percentage compared to T0 and T3 (P<0,05). T1 and T2 were also very significantly affecting the decreased of abnormality percentage compared to T0 and T3 (P<0,01). T1 generally gave the best result on the improvement of post-thawing motility and decreased the percentage of sperm mortality and abnormality.

show abstract

“…Malondialdehyde concentration in frozenthawed Nili-Ravi buffalo bull semen was assessed as described by Ahmed et al, (2018). Briefly semen was mixed with 8% (w/v) sodium dodecyl sulfate solution followed by addition of 20% (v/v) acetic acid.…”

Section: Catalase and Lipid Peroxidation Assessmentmentioning

confidence: 99%

Proximate Composition and Fatty Acid Profiles of Selected Fish Species From Pakistan

2020

THE JAPS

View full text Add to dashboard Cite

This study was designed to investigate the effect of L-lysine on post-thaw quality of Nili-Ravi buffalo bull semen. Twenty eight ejaculates were collected from four Nili-Ravi buffalo bulls. Semen samples were pooled and split into five equal aliquots and diluted in Tris-citric acid extender containing different concentrations of L-lysine (0.25, 0.5, 1 and 2mM). As a control, semen was extended without addition of L-lysine. The semen samples were cryo-preserved in liquid nitrogen. Post-thaw semen assessment revealed that sperm motility, viability, plasma membrane integrity, acrosomal integrity, mitochondrial integrity and DNA integrity were significantly improved (P ≤ 0.05) in 2mM L-lysine supplemented group as compared to control. For other groups supplemented with L-lysine; post-thaw motility, acrosomal integrity and mitochondrial integrity were also improved (P ≤ 0.05) for 1mM treated group as compared to control. DNA integrity was improved significantly by all L-lysine treated groups as compared to control. Malondialdehyde concentration was higher (P ≤ 0.05) in control group as compared to L-lysine treated groups. Significantly higher rate of catalase was recorded for 1mM and 2mM L-lysine treated groups as compared to control. In conclusion, the supplementation of 2mM L-lysine in semen extender can be used for cryopreservation of Nili-Ravi buffalo bull semen.

show abstract

Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Cited by 31 publications

References 24 publications

The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center

The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center

Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality

Proximate Composition and Fatty Acid Profiles of Selected Fish Species From Pakistan

Contact Info

Product

Resources

About