Effect of age on pyruvate kinase and lactate dehydrogenase distribution in rat kidney

Hb, Burch; Lowry, O. H.; Perry, SG; Fan, L. T.; Fagioli, Sabrina

doi:10.1152/ajplegacy.1974.226.5.1227

Cited by 21 publications

(4 citation statements)

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“…Our results concerning the localization of LPK in rat kidney cortex confirm those obtained by other authors using biochemical methods (Ibsen, 1977;Burch et al, 1974;Ibsen and Trippet, 1972;Jimenez de Asua, 1971). These authors, however, did not establish the striking difference in the L-PK pattern in kidney between male and female animals.…”

Section: Discussionsupporting

confidence: 91%

“…Mi-type pyruvate kinase (Mi-PK) is the main isoenzyme of skeletal and cardiac muscle and of brain Uimenez de Asua et al, 1971;Buechner and Pfleiderer, 1955), whereas the Mz-type PK (M2-PK) is present in most other tissues (Imamura and Enaka, 1972;Jimenez de Asua et al, 1971). The Ltype PK (LPK) occurs in liver (van Berkel et al, 1972) and in kidney proximal tubules (Reinacher and Eigenbrodt, 1981;Reinacher et al, 1979;Burch et al, 1974;Ibsen and Trippet, 1972;Jimenez de Asua et al, 1971). LPK has also been detected in the intestinal mucosa (Ogier et al, 1987;Saheki et al, 1979;Ostermann and Fritz, 1974).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistological demonstration of pyruvate kinase isoenzyme type L in rat with monoclonal antibodies.

Domingo¹,

Einig²,

Eigenbrodt³

et al. 1992

J Histochem Cytochem.

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Four monoclonal antibodies (MAb) specific for the Gtype isoenzyme of rat pyruvate kinase (GPK) were produced and characterized. They detect at least two different epitopes of the isoenzyme, as shown in interference binding assay and Western blot analysis after peptide mapping. The MAb were used in immunohistology to demonstrate the GPK isoenzyme in paraffin-embedded normal rat tissues. GPK was found only in hepatocytes, kidney proximal tubules, islet cells of pancreas, and epithelial cells of the villi of small intestine. The content of GPK in hepatocytes was often lower in the periportal areas as compared with the periveneous zone. In kidneys a dearcut difference in GPK content and distribution existed between male and female rats. Male animals possessed more GPK in the kidney cortex than females. The GPK content in the inner cortical zone (straight proximal tubules) was higher than in the outer cortical zone (convoluted proximal tubules) in kidneys of males. In contrast, female rats displayed less GPK in the inner than in the outer cortical zone of the kidneys. Only some of them exhibited the same amount of the isoenzyme in both parts of the kidney proximal tubules. (J Histochem Cytochem 40665473, 1992)

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Immunohistological demonstration of pyruvate kinase isoenzyme type L in rat with monoclonal antibodies.

Domingo¹,

Einig²,

Eigenbrodt³

et al. 1992

J Histochem Cytochem.

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“…Glycolysis is the metabolism of glucose into pyruvate, which can be further oxidized through the TCA cycle or metabolized into lactate. Glycolytic enzymes such as hexokinase, phosphofructokinase, and pyruvate kinase have the highest expression in thick ascending limbs and distal and collecting ducts [ 38 , 39 , 40 ]. Consistent with the enzyme expression levels, glucose oxidation and ATP generation from glucose in microdissected rat proximal tubules was significantly less than more distal tubule segments [ 41 , 42 ].…”

Section: Metabolism In the Healthy Kidneymentioning

confidence: 99%

Sugar or Fat? Renal Tubular Metabolism Reviewed in Health and Disease

Gewin

2021

Nutrients

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The kidney is a highly metabolically active organ that relies on specialized epithelial cells comprising the renal tubules to reabsorb most of the filtered water and solutes. Most of this reabsorption is mediated by the proximal tubules, and high amounts of energy are needed to facilitate solute movement. Thus, proximal tubules use fatty acid oxidation, which generates more adenosine triphosphate (ATP) than glucose metabolism, as its preferred metabolic pathway. After kidney injury, metabolism is altered, leading to decreased fatty acid oxidation and increased lactic acid generation. This review discusses how metabolism differs between the proximal and more distal tubular segments of the healthy nephron. In addition, metabolic changes in acute kidney injury and chronic kidney disease are discussed, as well as how these changes in metabolism may impact tubule repair and chronic kidney disease progression.

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“…Zusammenfassung:Die Glycolysekapazität in der Rattenniere wurde unter Bedingungen gesteigerter Gluconeogenese, wie nach 48 h Hunger und 48 h Acidose durch Sondenfütterung mit einer NH 4 Cl/Glucose-Lösung, untersucht. Ad libitum und durch eine Sonde mit Glucoselösung gefüt-terte Ratten dienten als Kontrollen.…”

Section: Unveränderte Glycolysekapazität In Der Rattenniere Unter Bedunclassified

Unchanged Glycolytic Capacity in Rat Kidney under Conditions of Stimulated Gluconeogenesis. Determination of Phosphofructokinase and Pyruvate Kinase in Microdissected Nephron Segments of Fasted and Acidotic Animals

Schmid¹,

Mall²,

Scholz³

et al. 1980

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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The glycolytic capacity in rat kidney was studied under conditions of stimulated gluconeogenesis like 48 h starvation and 48 h acidosis induced by force-feeding with a NH4Cl/glucose solution. Rats fed ad libitum and force-fed with a glucose solution served as controls. The specific activities of the key enzymes phosphofructokinase and pyruvate kinase were determined in single segments of the nephron. The determinations were performed by microdissection of lyophilized tissue sections followed by enzymatic analysis, including oil well technique, enzymatic amplification, and fluorometric measurement.In the distal portion of the nephron of both fed and force-fed animals a 10-fold higher specific activity of phosphofructokinase and pyruvate kinase was found compared with the proximal portion, known as the site of renal gluconeogenesis. Starvation and acidosis altered neither the distribution pattern nor the specific activities of these enzymes.From these results it is concluded that renal glycolysis does not undergo alterations at the level of enzymatic activities in favour of renal gluconeogenesis, probably because of the separate localization of both pathways within the nephron.

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Effect of age on pyruvate kinase and lactate dehydrogenase distribution in rat kidney

Cited by 21 publications

References 0 publications

Immunohistological demonstration of pyruvate kinase isoenzyme type L in rat with monoclonal antibodies.

Immunohistological demonstration of pyruvate kinase isoenzyme type L in rat with monoclonal antibodies.

Sugar or Fat? Renal Tubular Metabolism Reviewed in Health and Disease

Unchanged Glycolytic Capacity in Rat Kidney under Conditions of Stimulated Gluconeogenesis. Determination of Phosphofructokinase and Pyruvate Kinase in Microdissected Nephron Segments of Fasted and Acidotic Animals

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