Effect of ageing on CMV-specific CD8 T cells from CMV seropositive healthy donors

Pita-López, María Luisa; Gayoso, Inmaculada; DelaRosa, Olga; Casado, Javier G.; Alonso, Corona; Muñoz-Gomáriz, Elisa; Tarazona, Raquel; Solana, Rafael

doi:10.1186/1742-4933-6-11

Cited by 73 publications

(60 citation statements)

References 64 publications

(71 reference statements)

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“…This observation is consistent with previous observations showing decreased INFγ secretion in the PBMCs in elderly individuals (Olive et al 2001;Sester et al 2002). CMV-specific CD8 + T cells are increased in old individuals (65-101 years), but not in middle-aged/elderly (41-64 years) individuals, based on staining with HLA-A2 CMV pp65 pentamers (Pita-Lopez et al 2009). Another report demonstrated similar levels of EBV-and CMV-specific CD8 + T cells between young (young 20-61) and old (75-82 years) individuals, identified through HLA-A2 tetramers to EBV BMLF1 and CMV pp65 epitopes, respectively (Colonna-Romano et al 2007;Vescovini et al 2014).…”

Section: Cd8supporting

confidence: 82%

Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets

Stervbo

Bozzetti

Baron³

et al. 2015

AGE

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Immunosenescence results from a continuous deterioration of immune responses resulting in a decreased response to vaccines. A well-described age-related alteration of the immune system is the decrease of de novo generation of T and B cells. In addition, the accumulation of memory cells and loss of diversity in antigen specificities resulting from a lifetime of exposure to pathogens has also been described. However, the effect of aging on subsets of γδTCR + T cells and Tregs has been poorly described, and the efficacy of the recall response to common persistent infections in the elderly remains obscure. Here, we investigated alterations in the subpopulations of the B and T cells among 24 healthy young (aged 19-30)

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Section: Cd8supporting

confidence: 82%

Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets

Stervbo

Bozzetti

Baron³

et al. 2015

AGE

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“…It might be interesting to know whether these cells are a by product of timeenduring stimulation by an unknown, infectious agent. One possible candidate would be CMV, since Chidrawar et al (2009) have demonstrated that CMV infection in the elderly influences not only T lymphocytes (Pita-Lopez et al 2009;Pawelec et al 2009Pawelec et al , 2010, but also negatively affects B cells.…”

Section: Discussionmentioning

confidence: 99%

B cell immunosenescence: different features of naive and memory B cells in elderly

et al. 2011

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Elderly people show a reduced protection against new infections and a decreased response to vaccines as a consequence of impairment of both cellular and humoral immunity. In this paper we have studied memory/naïve B cells in the elderly, evaluating surface immunoglobulin expression, production of the pro-and anti-inflammatory cytokines, tumor necrosis factor (TNF)-a and interleukin (IL)-10, and presence of somatic hypermutation, focusing on the IgG ? IgD -CD27 -double negative (DN) B cells that are expanded in the elderly. Our results show that naïve B cells from young donors need a sufficiently strong stimulus to be activated ''in vitro'', while naïve B cells from old subjects are able to produce IL-10 and TNF-a when stimulated ''physiologically'' (a-CD40/IL-4), suggesting that these cells might play a role in the control of the immuno-inflammatory environment in the elderly. In addition, in the elderly there is an accumulation of DN B cells with a reduced rate of somatic hypermutation. Thus, DN B lymphocytes may be exhausted cells that are expanded and accumulate as a by-product of persistent stimulation or impaired germinal center formation.

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“…The most significant age‐related change in the human immune system is the quality and phenotype of the cytotoxic CD8 T‐cell subset. Indeed, with age, and in chronic infections such as human immunodeficiency virus (Appay et al ., 2007) and cytomegalovirus (Pita‐Lopez et al ., 2009), the majority of CD8 + T cells become antigen‐experienced and acquire a deregulated, pro‐inflammatory phenotype. These late‐differentiated CD8 + T cells possess many features of replicative senescence that have been characterized in long‐term in vitro cultures (Signer et al ., 2008).…”

Section: Cellular Senescence and Immune Cell Fate Decisionmentioning

confidence: 99%

Cellular senescence impact on immune cell fate and function

et al. 2016

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SummaryCellular senescence occurs not only in cultured fibroblasts, but also in undifferentiated and specialized cells from various tissues of all ages, in vitro and in vivo. Here, we review recent findings on the role of cellular senescence in immune cell fate decisions in macrophage polarization, natural killer cell phenotype, and following T‐lymphocyte activation. We also introduce the involvement of the onset of cellular senescence in some immune responses including T‐helper lymphocyte‐dependent tissue homeostatic functions and T‐regulatory cell‐dependent suppressive mechanisms. Altogether, these data propose that cellular senescence plays a wide‐reaching role as a homeostatic orchestrator.

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Effect of ageing on CMV-specific CD8 T cells from CMV seropositive healthy donors

Cited by 73 publications

References 64 publications

Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets

Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets

B cell immunosenescence: different features of naive and memory B cells in elderly

Cellular senescence impact on immune cell fate and function

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