Effect of Aging on Growth and Flowering of the Terminal Bud of Pharbitis nil

Owens, Vivian; Paolillo, Dominick J.

doi:10.2307/2444300

Cited by 3 publications

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“…In similar experiments, maximal expression of terminal flower buds was not observed until seedlings were 6 days old (equivalent to 5 DPI in our experiments) at the time of induction (King and Evans 1969) or 4 DPI according to Owens and Paolillo (1986b). Likewise, maximal flowering (100% of the individuals flowering) was obtained only with seedlings equivalent to 4 DPI or older when grown under CL prior to a 16 h dark inductive period (Marushige and Takimoto 1967).…”

Section: Influence Of Seedling Age On the Synchrony Of The Floral Ressupporting

confidence: 70%

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

Bassett

Mothershed

Galau

1991

J Plant Growth Regul

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Abstract. An unknown substance(s) produced in the cotyledons of seedlings of the Japanese morning glory (Pharbitis nil) during a defined period of darkness triggers the subsequent initiation of floral buds at apical and axillary meristems. Recent studies have concentrated on characterizing molecular changes as a possible mechanism associated with its synthesis, but these have failed to eliminate interference due to lack of development unity in the sampled population and to consider different kinetic alternatives of those potential changes. The current study demonstrates that numerous age-related changes occur in polypeptides from cotyledons during growth under noninductive conditions, but that these are minimal in older seedlings selected for improved synchrony of the floral response. Polypeptides from older seedlings sampled at various times during and after a dark inductive period were examined by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). No differences associated with floral induction could be detected. The results indicate that changes in gene expression correlated with floral-induction do not occur in the abundant polypeptide fraction within the limitations of the techniques employed.Floral initiation in many plants is triggered by changes in day length, a process termed photoperiodic induction, and is believed to result from the synthesis of a substance (or substances) within the leaves and cotyledons of the induced plant (for a review see Kinet et al. 1985). Although many attempts have been made to isolate and identify this floral-inducing substance, no success has been reported to date. Changes in gene expression have been proposed as one possible mechanism regulating or accompanying the synthesis of a floralinducing substance. Recent studies have supported this hypothesis in a number of experimental systems (Kannangara et al. 1990, Lay-Yee et al. 1987a,b, Warm 1984. However, other researchers have failed to find evidence for rapid changes in gene expression during induction (Friedman et al. 1987, Kimpel and Doss I989, Ono et al. 1988), suggesting that evidence testing this hypothesis and perhaps the hypothesis itself should be critically reevaluated.We have examined polypeptides present in vivo in cotyledons of the Japanese morning glory cv. Violet during growth and floral induction. The aim of the growth studies was to catalogue age-related differences in polypeptide profiles of seedlings that are capable of responding to photoperiodic induction and to determine morphological markers (after Lamoreaux et al. 1978) that correlate with these differences as criteria for selecting populations with improved synchrony. These populations were then used to examine cotyledon polypeptides during photoperiodic induction. The goal of these studies was to confirm whether or not changes in gene expression accompany this process, as some have reported, including changes that might arise as a consequence of posttranslational modification. We find no evidence of photoinduction-specific changes in t...

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Section: Influence Of Seedling Age On the Synchrony Of The Floral Ressupporting

confidence: 70%

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

Bassett

Mothershed

Galau

1991

J Plant Growth Regul

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BOUGAINVILLEA INFLORESCENCE MERISTEM DEVELOPMENT: COMPARATIVE ACTION OF GA₃ IN VIVO AND IN VITRO

Steffen

Sachs

Hackett

1988

American J of Botany

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Although gibberellic acid (GA3) promotes stem elongation and inhibits flowering in Bougainvillea, no inhibition of floret initiation was observed when excised meristems were cultured in vitro in media containing GA. These results are predicted by a nutrient diversion hypothesis in which GA‐induced inhibition of flower initiation in intact plants is considered an indirect result of growth promotion of competing vegetative sinks with consequent diversion of nutrients away from the inflorescence meristem. The failure to observe GA‐induced inhibition of floret initiation in vitro was probably the result of an excess of metabolites for growth and differentiation of the excised meristems in the media.

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Roles of Photosynthesis and Assimilate Partitioning in Flower Initiation

Sachs¹

1987

Manipulation of Flowering

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Effect of Aging on Growth and Flowering of the Terminal Bud of Pharbitis nil

Cited by 3 publications

References 0 publications

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

BOUGAINVILLEA INFLORESCENCE MERISTEM DEVELOPMENT: COMPARATIVE ACTION OF GA₃ IN VIVO AND IN VITRO

Roles of Photosynthesis and Assimilate Partitioning in Flower Initiation

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Effect of Aging on Growth and Flowering of the Terminal Bud of Pharbitis nil

Cited by 3 publications

References 0 publications

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

BOUGAINVILLEA INFLORESCENCE MERISTEM DEVELOPMENT: COMPARATIVE ACTION OF GA3 IN VIVO AND IN VITRO

Roles of Photosynthesis and Assimilate Partitioning in Flower Initiation

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Product

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BOUGAINVILLEA INFLORESCENCE MERISTEM DEVELOPMENT: COMPARATIVE ACTION OF GA₃ IN VIVO AND IN VITRO