Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor

Maiorano, Alfredo Eduardo; Silva, Elda Sabino da; Ottoni, Cristiane Angélica; Piccoli, Rosane A. M.; Fernandez, Rubén Cuervo; Maresma, Beatriz Guilarte; Rodrigues, Maria Filomena de Andrade

doi:10.1007/s10529-020-03006-9

Cited by 15 publications

(5 citation statements)

References 48 publications

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“…Therefore, all further fermentation experiments with the tested strains were incubated for 6 days. Depending on the microbe cultivated and the bio-product studied, increasing agitation speed has also been reported to reduce or promote productivity ( 54 , 55 ). In another study by Saad et al, their experimental results revealed that lipid and GLA content were increased in samples agitated at 600 and 440 rpm, with values as high as 38.71% and 0.058 (g/g), respectively, in oleaginous fungus Cunninghamella bainieri 2A1 ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production in Rhizomucor pusillus and Mucor circinelloides

et al. 2022

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In this study, 18 standard amino acids were tested as a single nitrogen source on biomass, total lipid, total fatty acid (TFA) production, and yield of γ-linolenic acid (GLA) in Rhizomucor pusillus AUMC 11616.A and Mucor circinelloides AUMC 6696.A isolated from unusual habitats. Grown for 4 days at 28°C, shaking at 150 rpm, the maximum fungal biomass for AUMC 6696.A was 14.6 ± 0.2 g/L with arginine and 13.68 ± 0.1 g/L with asparagine, when these amino acids were used as single nitrogen sources, while AUMC 11616.A maximum biomass was 10.73 ± 0.8 g/L with glycine and 9.44 ± 0.6 g/L with valine. These were significantly higher than the ammonium nitrate control (p < 0.05). The highest levels of TFA were achieved with glycine for AUMC 11616.A, 26.2 ± 0.8% w/w of cell dry weight, and glutamic acid for AUMC 6696.A, 23.1 ± 1.3%. The highest GLA yield was seen with proline for AUMC 11616.A, 13.4 ± 0.6% w/w of TFA, and tryptophan for AUMC 6696.A, 12.8 ± 0.3%, which were 38% and 25% higher than the ammonium tartrate control. The effects of environmental factors such as temperature, pH, fermentation time, and agitation speed on biomass, total lipids, TFA, and GLA concentration of the target strains have also been investigated. Our results demonstrated that nitrogen assimilation through amino acid metabolism, as well as the use of glucose as a carbon source and abiotic factors, are integral to increasing the oleaginicity of tested strains. Few studies have addressed the role of amino acids in fermentation media, and this study sheds light on R. pusillus and M. circinelloides as promising candidates for the potential applications of amino acids as nitrogen sources in the production of lipids.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production in Rhizomucor pusillus and Mucor circinelloides

et al. 2022

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“…The transfructosylation activity ( A T ) was determined by incubating 0.1 mL of the soluble enzyme (culture broth) or 1 g of the enzymes immobilized on PHB and GLU‐PHB with 3.7 mL of a sucrose solution (47%, w v −1 ) and 1.2 mL of tris‐acetate buffer at 0.2 mol L −1 and pH 5.5. The reaction was performed in a shaking water bath at 50 °C and 190 rpm for 60 min and stopped by incubation in boiling water for 10 min and then an ice bath for 5 min 4,5,7,40 . The reaction medium was vacuum‐filtered and the concentrations of reducing sugars and glucose were quantified by the methods DNS and GOD‐PAP, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The reaction was performed in a shaking water bath at 50 °C and 190 rpm for 60 min and stopped by incubation in boiling water for 10 min and then an ice bath for 5 min. 4,5,7,40 The reaction medium was vacuum-filtered and the concentrations of reducing sugars and glucose were quantified by the methods DNS and GOD-PAP, respectively. The unit of transfructosylation activity was defined as the amount of enzyme that transfers 1 μmol of fructose per minute under the chosen experimental conditions.…”

Section: Standard Enzymatic Activity Assaymentioning

confidence: 99%

Enhancement of fructosyltransferase stability by immobilization on polyhydroxybutyrate and glutaraldehyde‐activated polyhydroxybutyrate for fructooligosaccharides production

Araújo

Becalette

Silva

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Fructosyltransferase (FTase) enzyme is an important biocatalyst for fructooligossaccaride (FOS) production, but it has low stability in soluble form. The aim of this work was to analyze the stability and kinetics of an fructosyltransferase (FTase E.C.2.4.1.9) from Aspergillus oryzae IPT‐301 immobilized on polyhydroxybutyrate (PHB) and glutaraldehyde‐activated polyhydroxybutyrate (GLU‐PHB). RESULTS The immobilization yields of the FTase on PHB and GLU‐PHB were about 41% and 55%, respectively. The recovery activities of the FTase immobilized on PHB and GLU‐PHB were about 17% and 11%, respectively. At 30 °C, the FTase immobilized on GLU‐PHB showed a half‐life 2.6 and 1.4 times higher than the soluble FTase and the FTase immobilized on PHB, respectively. The FTase immobilized on PHB and GLU‐PHB retained more than 40% and 55% of their initial activity after six sequential reaction batches, respectively. Both immobilized enzymes showed kinetic behaviors that were fitted by the Hill model, with transfructosylation activity maximum at concentrations between 400 g substrate L−1 and 500 g substrate L−1. CONCLUSION These results demonstrate that the FTase immobilized on PHB and, especially, on GLU‐PHB, are promising biocatalysts for FOS production in heterogeneous reaction systems. © 2022 Society of Chemical Industry (SCI).

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“…Other benefits associated with FOS consumption are reduction of serum cholesterol, increased absorption of calcium and magnesium, prevention of colon cancer, and production of B vitamins. In addition, FOS are non-cariogenic sugars, low in calories, sweet tasting and exhibit antioxidant activity through free radical scavenging (Perna et al, 2018;Cunha et al, 2019;Kaplan and Hutkins, 2000;Pereira and Gibson, 2002;Maiorano et al, 2020;Faria et al, 2021). Among the FOS, 1-kestose has shown strong bifidogenic activity which has been associated with multiple beneficial effects on the host, such as an increase in cecal butyrate level and a decrease in serum insulin level (Tochio et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Validation of an Analytical Method for the Quantification of 1-Kestose, Glucose and Sucrose in the Reaction Products of a Fructosyltransferase

Martínez,

Veitia,

Brizuela

et al. 2023

J microb biotech food sci

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The enzymatic synthesis of fructooligosaccharides (FOS) from sucrose by the action of fructosyltransferases produces a mixture of different carbohydrates. The concentration of each carbohydrate involved must be quantified to follow its variation as the kinetics of the reaction progresses. This requires a method such as high-performance liquid chromatography (HPLC) with refractive index detector (IR). The aim of this work was to validate the HPLC-IR analytical method for the quantification of total and individual carbohydrates in mixtures of 1-kestose, sucrose and glucose. For this study, the validation parameters recommended by the FDA (Food and Drug Administration), the ICH (International Council for Harmonisation) and the EMEA (European Medicines Agency) were followed. The method was able to quantify carbohydrates with adequate levels of precision, accuracy and linearity in ranges of 1.6-8.7, 4.3-22.0 and 7.0-41.0 mg/mL for glucose, 1-kestose and sucrose, respectively. The lowest limit of detection was for glucose (0.2 mg/mL) and the highest for sucrose (0.8 mg/mL), while the lowest and highest limit of quantification were also for glucose (0.6 mg/mL) and sucrose (1.8 mg/mL). The detection (0.7 mg/mL) and quantification (1.4 mg/mL) limits for 1-kestose were intermediate. The HPLC-IR method was used to calculate the concentration of the carbohydrates involved in the reaction kinetics of a recombinant fructosyltransferase (Sa1-SSTrec). This analytical technique can be implemented for the analysis of samples from the FOS production process at both laboratory and industrial scale, but also the technique is useful to detect 1-kestose in functional foods based in fruit juices.

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Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor

Cited by 15 publications

References 48 publications

Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production in Rhizomucor pusillus and Mucor circinelloides

Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production in Rhizomucor pusillus and Mucor circinelloides

Enhancement of fructosyltransferase stability by immobilization on polyhydroxybutyrate and glutaraldehyde‐activated polyhydroxybutyrate for fructooligosaccharides production

Validation of an Analytical Method for the Quantification of 1-Kestose, Glucose and Sucrose in the Reaction Products of a Fructosyltransferase

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