Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis

Gomes, Carolina Cintra; Freitas, Deborah Queiroz; Araújo, Ana Lucélia de; Ramírez‐Sotelo, Laura Ricardina; Yamamoto‐Silva, Fernanda Paula; Silva, Brunno Santos de Freitas; Távora, Débora de Melo; Almeida, Solange María de

doi:10.1016/j.joms.2017.11.019

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…The findings of the present study suggest that although BV/TV, Tb.Th, and Tot.Po were low, there was no further decrease in bone mass or micro-architecture, relaying that ALN is able to prevent the bone deterioration caused by ovariectomy. This is in line with both the studies of Gomes et al and Romualdo et al [ 34 , 71 ].…”

Section: Discussionsupporting

confidence: 93%

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

Chatterjee

Faot

Correa

et al. 2021

IJMS

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The aim of the study was to quantify the micro-architectural changes of the jaw bone in response to ovariectomy, exposed or not to bisphosphonate treatment. A total of 47 Wistar rats were ovariectomized (OVX) or sham-operated (shOVX) and exposed to osteoporosis preventive treatment for eight weeks either with bisphosphonates (alendronate, ALN; group OVX-ALN) three days/week at a dose of 2 mg/kg or with saline solution (untreated control condition; group OVX). The bone morphometric parameters of the trabecular jaw bone were assessed using ex vivo micro-computed tomography. The regions of interest investigated in the maxilla were the inter-radicular septum of the second molar and the tuber. The regions quantified in the mandible included the three molar regions and the condyle. A one-way analysis of variance followed by pairwise comparison using Tukey’s HSD and the Games–Howell test was conducted to explore significant differences between the groups. In the maxilla, OVX decreased the bone volume in the inter-radicular septum of the second molar. Bisphosphonate treatment was able to prevent this deterioration of the jaw bone. The other investigated maxillary regions were not affected by (un)treated ovariectomy. In the mandible, OVX had a significant negative impact on the jaw bone in the buccal region of the first molar and the inter-radicular region of the third molar. Treatment with ALN was able to prevent this jaw bone loss. At the condyle site, OVX significantly deteriorated the trabecular connectivity and shape, whereas preventive bisphosphonate treatment showed a positive effect on this trabecular bone region. No significant results between the groups were observed for the remaining regions of interest. In summary, our results showed that the effects of ovariectomy-induced osteoporosis are manifested at selected jaw bone regions and that bisphosphonate treatment is capable to prevent these oral bone changes.

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Section: Discussionsupporting

confidence: 93%

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

Chatterjee

Faot

Correa

et al. 2021

IJMS

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“…Then 3D and 2D analyses were performed using a software analyzer. Bone microstructure analysis was conducted in the region of interest, and finally, bone mineral density (BMD), bone mineral content (BMC), trabecular bone volume fraction (BV/TV), trabecular thickness (Tb.Th), bone surface-to-volume ratio (BS/BV), and trabecular separation (Tb.Sp) were determined [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

MiR-210 improves postmenopausal osteoporosis in ovariectomized rats through activating VEGF/Notch signaling pathway

Ren

Zhu

Tan

et al. 2023

BMC Musculoskelet Disord

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Background To explore the effect and mechanism of action of miR-210 on postmenopausal osteoporosis (PMPO) in ovariectomized rats in vivo. Methods An ovariectomized (OVX) rat model was established by ovariectomy. Tail vein injection was performed to overexpress and knock down miR-210 in OVX rats, followed by the collection of blood and femoral tissues from each group of rats. And quantitative real-time polymerase chain reaction (qRT-PCR) was applied to assess the expression level of miR-210 in femoral tissues of each group. Micro computed tomography (Micro CT) was adopted to scan the microstructure of the femoral trabecula in each group to obtain relevant data like bone mineral density (BMD), bone mineral content (BMC), trabecular bone volume fraction (BV/TV), trabecular thickness (Tb.Th), bone surface-to-volume ratio (BS/BV), and trabecular separation (Tb.Sp). ELISA was used for determining the level of bone alkaline phosphatase (BALP), amino-terminal propeptide of type I procollagen (PINP), osteocalcin (OCN), and C-terminal telopeptide of type I collagen (CTX-1) in serum; and Western blot for the protein level of Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and collagen type I alpha 1 (COL1A1) in femoral tissues. Results MiR-210 expression was significantly decreased in femoral tissues of OVX rats. Overexpression of miR-210 could obviously increase BMD, BMC, BV/TV and Tb.Th, whereas significantly decrease BS/BV and Tb.Sp in femurs of OVX rats. Moreover, miR-210 also downregulated BALP and CTX-1 level, upregulated PINP and OCN level in the serum of OVX rats promoted the expression of osteogenesis-related markers (Runx2, OPN and COL1A1) in the femur of OVX rats. Additionally, further pathway analysis revealed that high expression of miR-210 activated the vascular endothelial growth factor (VEGF)/Notch1 signaling pathway in the femur of OVX rats. Conclusion High expression of miR-210 may improve the micromorphology of bone tissue and modulate bone formation and resorption in OVX rats by activating the VEGF/Notch1 signaling pathway, thereby alleviating osteoporosis. Consequently, miR-210 can serve as a biomarker for the diagnosis and treatment of osteoporosis in postmenopausal rats.

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“…The low-resolution MDCT is considered to be the input image

. Some high-resolution images such as HR-pQCT [ 71 ], micro-CT [ 72 , 73 , 74 ], etc., can be treated as the mapping targets

. In this paper, we only consider generating micro-CT-like images, so we set

, and the micro-CT image is the only

.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Possibility of Measuring Vertebrae Bone Structure Metrics Using MDCT Images: An Unpaired Image-to-Image Translation Method

2023

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Bone structure metrics are vital for the evaluation of vertebral bone strength. However, the gold standard for measuring bone structure metrics, micro-Computed Tomography (micro-CT), cannot be used in vivo, which hinders the early diagnosis of fragility fractures. This paper used an unpaired image-to-image translation method to capture the mapping between clinical multidetector computed tomography (MDCT) and micro-CT images and then generated micro-CT-like images to measure bone structure metrics. MDCT and micro-CT images were scanned from 75 human lumbar spine specimens and formed training and testing sets. The generator in the model focused on learning both the structure and detailed pattern of bone trabeculae and generating micro-CT-like images, and the discriminator determined whether the generated images were micro-CT images or not. Based on similarity metrics (i.e., SSIM and FID) and bone structure metrics (i.e., bone volume fraction, trabecular separation and trabecular thickness), a set of comparisons were performed. The results show that the proposed method can perform better in terms of both similarity metrics and bone structure metrics and the improvement is statistically significant. In particular, we compared the proposed method with the paired image-to-image method and analyzed the pros and cons of the method used.

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Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis

Cited by 5 publications

References 22 publications

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

MiR-210 improves postmenopausal osteoporosis in ovariectomized rats through activating VEGF/Notch signaling pathway

Exploring the Possibility of Measuring Vertebrae Bone Structure Metrics Using MDCT Images: An Unpaired Image-to-Image Translation Method

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