Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat

Kayalı, Refik; Çakatay, Ufuk; Akçay, Tülay; Altuğ, Tuncay

doi:10.1002/cbf.1190

Cited by 176 publications

(92 citation statements)

References 41 publications

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“…Kayali et al [93] found that LA administration caused an increase in protein carbonyl and nitrotyrosine levels and a decrease in total thiol, non-protein thiol, and lipid hydroperoxide levels in the brain tissue of aged rats. The authors assumed prooxidative effects of LA in the brain tissue of aged rats may be due to the prooxidant effects of LA [93]. However, the results should be further confirmed with more convincing parameters and methods.…”

Section: Antioxidant Defensementioning

confidence: 98%

The Effects and Mechanisms of Mitochondrial Nutrient α-Lipoic Acid on Improving Age-Associated Mitochondrial and Cognitive Dysfunction: An Overview

2007

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We have identified a group of nutrients that can directly or indirectly protect mitochondria from oxidative damage and improve mitochondrial function and named them "mitochondrial nutrients". The direct protection includes preventing the generation of oxidants, scavenging free radicals or inhibiting oxidant reactivity, and elevating cofactors of defective mitochondrial enzymes with increased Michaelis-Menten constant to stimulate enzyme activity, and also protect enzymes from further oxidation, and the indirect protection includes repairing oxidative damage by enhancing antioxidant defense systems either through activation of phase 2 enzymes or through increase in mitochondrial biogenesis. In this review, we take alpha-lipoic acid (LA) as an example of mitochondrial nutrients by summarizing the protective effects and possible mechanisms of LA and its derivatives on age-associated cognitive and mitochondrial dysfunction of the brain. LA and its derivatives improve the age-associated decline of memory, improve mitochondrial structure and function, inhibit the age-associated increase of oxidative damage, elevate the levels of antioxidants, and restore the activity of key enzymes. In addition, co-administration of LA with other mitochondrial nutrients, such as acetyl-L: -carnitine and coenzyme Q10, appears more effective in improving cognitive dysfunction and reducing oxidative mitochondrial dysfunction. Therefore, administrating mitochondrial nutrients, such as LA and its derivatives in combination with other mitochondrial nutrients to aged people and patients suffering from neurodegenerative diseases, may be an effective strategy for improving mitochondrial and cognitive dysfunction.

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Section: Antioxidant Defensementioning

confidence: 98%

The Effects and Mechanisms of Mitochondrial Nutrient α-Lipoic Acid on Improving Age-Associated Mitochondrial and Cognitive Dysfunction: An Overview

2007

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“…Advanced oxidation protein product (AOPP) levels were determined according to the method of Kayali et al (2006). Briefly, 0.4 ml of lung extract supernatant was treated with 0.8-ml phosphate buffer (0.1 M; pH 7.4).…”

Section: Aopp Levelsmentioning

confidence: 99%

Dietary unsaponifiable fraction of extra virgin olive oil supplementation attenuates lung injury and DNA damage of rats co-exposed to aluminum and acrylamide

Ghorbel

Chaâbane

Boudawara

et al. 2016

Environ Sci Pollut Res

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Aluminum chloride (AlCl3) and acrylamide (ACR) are well known as environmental pollutants inducing oxidative stress. Our study investigated the effects of these contaminants and if the hydrophilic fraction of extra virgin olive oil was able to prevent lung oxidative stress and DNA damage. Animals were divided into four groups of six each: group 1, serving as controls, received distilled water; group 2 received in drinking water aluminum chloride (50 mg/ kg body weight) and by gavage acrylamide (20 mg/kg body weight); group 3 received both aluminum and acrylamide in the same way and the same dose as group 2 and hydrophilic fraction from olive oil (OOHF) (1 ml) by gavage; group 4 received only OOHF by gavage. Exposure of rats to both aluminum and acrylamide provoked oxidative stress in lung tissue based on biochemical parameters and histopathological alterations. In fact, we have observed an increase in malondialdehyde (MDA), H2O2, and advanced oxidation protein product (AOPP) and a decrease in reduced glutathione (GSH), non-protein thiols (NPSH), and vitamin C levels. Activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also decreased. Histopathological changes in lung tissue were noted like emphysema, vascular congestion, and infiltration of inflammatory cells. A random DNA degradation was observed on agarose gel in the lung of AlCl3 and acrylamide (ACR)-treated rats. Co-administration of OOHF to treated rats improved biochemical parameters to near control values and lung histoarchitecture. The smear formation of genomic DNA was reduced. The hydrophilic fraction of extra virgin olive oil might provide a basis for developing a new dietary supplementation strategy in order to prevent lung tissue damage.

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“…Tissue samples (200 mg) were homogenized manually in 2 ml of homogenizing bu er (100 mM KH2PO4-K2HPO4, pH 7.4, plus 0.1% (w/v) digitonin) in a glass homogenizer to avoid disruption of nuclear membranes. In this way, contamination by nucleic acids was minimized [12]. Homogenates obtained from the rats were centrifuged at 5000 × g for 10 min, and the various analytes were assayed using the supernatant fraction.…”

Section: Preparation Of Tissue Samplesmentioning

confidence: 99%

Effects of Hormone Replacement Therapy on Plasma and Tissue Fibrinolytic Activity in a Rat Model of Surgically Induced Menopause

Topçuoğlu¹,

Albayrak²,

Erman³

et al. 2014

CIM

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Purpose: e purpose of this study was to analyze the e ects of estrogen de ciency and hormone replacement therapy (HRT) on brinolytic activity in a rat mode of surgicallyinduced menopause.Methods: Twelve-week-old, sexually mature female Sprague-Dawley rats, each weighing 200-250 g, were randomly divided into four groups: (1) sham-operated group, (2) ovariectomy group, (3) ovariectomy group followed by oral administration of daily 17β-estradiol (0.02 mg/kg/day) (E2) + norethisterone acetate (0.01 mg/kg/day), and (4) ovariectomy group followed by oral administration of daily 17β-estradiol (0.01 mg/kg/ day) + drospirenone (0.02 mg/kg/day). Tissue plasminogen activator (tPA) antigen, plasminogen activator inhibitor-1 (PAI-1) antigen, and PAI-1/tPA levels were measured as markers of brinolysis in plasma and liver and brain tissue.Results: Compared with sham-operated rats, ovariectomized rats showed higher levels of brinolytic activity; however, the increased brinolytic activity in plasma and liver tissue was signi cantly reduced by HRT regimens. No change was observed in the levels of brinolytic activity in brain tissue.Conclusions: HRT showed bene cial e ects by decreasing brinolytic activity related to surgically-induced menopause. Short-term HRT treatment was associated with a shi in the procoagulant-anticoagulant balance toward a procoagulant state.

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Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat

Cited by 176 publications

References 41 publications

The Effects and Mechanisms of Mitochondrial Nutrient α-Lipoic Acid on Improving Age-Associated Mitochondrial and Cognitive Dysfunction: An Overview

The Effects and Mechanisms of Mitochondrial Nutrient α-Lipoic Acid on Improving Age-Associated Mitochondrial and Cognitive Dysfunction: An Overview

Dietary unsaponifiable fraction of extra virgin olive oil supplementation attenuates lung injury and DNA damage of rats co-exposed to aluminum and acrylamide

Effects of Hormone Replacement Therapy on Plasma and Tissue Fibrinolytic Activity in a Rat Model of Surgically Induced Menopause

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