Effect of aluminum and pH on the growth ofAnacystis nidulans

Lee, Lee H.; Lustigman, B.; Chu, I-Yu; Jou, Huey-Ling

doi:10.1007/bf01689958

Cited by 30 publications

(3 citation statements)

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“…4, green line). The pH of S. IU 625 cultures grown in the absence (Lee et al, 1991) or presence (Lee et al, 2002) of mercuric chloride is within the range of pH 7-10. Mercuric chloride has been shown to be stable in solution up to a pH as high as 10.5 (Spence and Barton, 2003).…”

Section: Resultsmentioning

confidence: 99%

“…They also serve as good indicators of environmental pollution, especially heavy metal contamination. Studies have found that heavy metals such as barium, aluminum, lead, cadmium, and mercury are toxic to S. IU 625 (Lee et al, 1991, 1992a, 1992b; Lee and Lustigman, 1996). However, even essential metals such as Mn 2+ , Cu 2+ , Zn 2+ and Ni 2+ inhibit the growth of S. IU 625 when present at high concentrations (Lee et al, 1993, 1994; Lee and Lustigman, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Adaption of Synechococcus sp. IU 625 to growth in the presence of mercuric chloride

et al. 2012

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Resistance to heavy metals is important for the survival of bacteria in contaminated environments. In this study, we show that the unicellular cyanobacterial species Synechococcus sp. IU 625 adapts to growth in the presence of mercuric chloride, recovering from pigmentation and morphological defects. Cells accumulate mercury within two hours of growth and by three days, the total mercury concentration is significantly reduced, with all remaining mercury associated with the cells. This suggests that Synechococcus sp. IU 625 can convert mercury to a volatile form.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adaption of Synechococcus sp. IU 625 to growth in the presence of mercuric chloride

et al. 2012

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“…This moiety, which is associated with this trivalent metal from the early stages of growth, is involved in the formation of a gelatinous precipitate in which aluminum is eventually immobilized. Although the toxicity of this trivalent element is well documented, reports of tolerance mechanisms to aluminum are rare (9,18). Aluminum binds to membrane components of cell walls and is also known to interfere with iron and calcium metabolism.…”

Section: Discussionmentioning

confidence: 99%

Aluminum Elicits Exocellular Phosphatidylethanolamine Production in Pseudomonas fluorescens

Appanna¹,

Pierre²

1996

Appl Environ Microbiol

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Pseudomonas fluorescens ATCC 13525 was found to grow in a minimal mineral medium supplemented with millimolar amounts of aluminum, a known environmental toxicant. During the stationary phase of growth, the trivalent metal was localized in a phosphatidylethanolamine (PE)-containing residue. The concentration of PE in pellets ranged from 1.7 to 13.9 mg ml of culture ؊1 in media supplemented with 1 to 30 mM aluminum. Although the gelatinous residue was observed during the stationary phase of growth, ultracentrifugation and dialysis experiments revealed that PE was produced from earlier stages of incubation and was associated with aluminum. A sharp diminution in the levels of PE and aluminum in the spent fluid was concomitant with the formation of the insoluble deposit. The aluminum content of the soluble cellular fraction increased during growth and reached an optimum of 1.85 mM of test metal at 45 h in cultures with 15 mM aluminum. Further incubation, however, led to a marked decrease in the cellular aluminum content, and during the stationary phase of growth, only trace amounts of the trivalent metal were detected in this fraction. When 45-h cells were incubated in fresh citrate medium, most of the intracellular aluminum was secreted in the spent fluid and citrate was rapidly consumed. Aluminum efflux was also observed in cultures in which D-glucose was substituted for citrate. However, no efflux of this trivalent metal was evident in media devoid of either citrate or D-glucose. Scanning electron microscopic studies and X-ray energy-dispersive analyses of the dialyzed supernatant aided in the visualization of nodule-like aluminum-and phosphorus-rich bodies associated with thread-like carbon-, oxygen-, and phosphorus-containing structures. Transmission electron microscopic and electron energy loss spectroscopic analyses revealed the presence of aluminum within bacteria after 45 h of incubation. Cells harvested after aluminum insolubilization did not shown aluminum inclusions. This aluminum-tolerant microbe may have potential application in bioremediation processes.

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