Effect of Ambroxol on Secretion, Production and Gene Expression of Mucin from Cultured Airway Epithelial Cells

Abstract

In this study, we investigated whether ambroxol signifi cantly affects secretion, production and gene expression of mucin from cultured airway epithelial cells. Confl uent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then treated for 30 min with ambroxol to assess the effect on mucin secretion using ELISA. Additionally, confl uent NCI-H292 cells were pretreated with ambroxol for 30 min and then stimulated with EGF or PMA for 24 h. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1)

Inhibitory Effect of an Urotensin II Receptor Antagonist on Proinflammatory Activation Induced by Urotensin II in Human Vascular Endothelial Cells

“…The expressions of adhesion molecules, cytokines, and TF mRNA were measured using RT-PCR as previously described (Lee et al ., 2011) . cDNAs were synthesized from total RNA using M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA).…”