Effect of Amino Acid Availability on Vitamin B
            <sub>12</sub>
            Production in
            <i>Lactobacillus reuteri</i>

Santos, F.D.; Teusink, Bas; Molenaar, Douwe; Heck, Maurice van; Wels, Michiel; Sieuwerts, Sander; Vos, Willem M. de; Hugenholtz, Jeroen

doi:10.1128/aem.02487-08

Cited by 31 publications

(33 citation statements)

References 21 publications

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“…These altered transcriptional activities probably arise because the incomplete AMC cycle in the mutant prevents sufficient production of cysteine by the reverse trans-sulfuration pathway. Similar transcriptional patterns have been observed in L. reuteri JCM112 grown in the absence of cysteine (11). These met- the genes encoding the enzymes that catalyze these reactions were upregulated in the luxS mutant under all growth conditions.…”

Section: Resultssupporting

confidence: 64%

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

et al. 2012

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c Autoinducer-2 (AI-2)-mediated quorum sensing has been extensively studied in relation to the regulation of microbial behavior. There are, however, two potential roles for the AI-2 synthase (LuxS). The first is in the production of AI-2 and the second is as an enzyme in the activated methyl cycle, where it catalyzes the conversion of S-ribosylhomocysteine to homocysteine. The by-product of the reaction catalyzed by LuxS is (S)-4,5-dihydroxy-2,3-pentanedione, which spontaneously forms the furanones known collectively as AI-2. The mammalian gut contains a complex collection of bacterial species so a method of interspecies communication might influence community structure and function. Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent forestomach, where it adheres to the nonsecretory epithelium, forming a biofilm. Microarray comparisons of gene expression profiles of the L. reuteri 100-23 wild type and a luxS mutant under different culture conditions revealed altered transcription of genes encoding proteins associated with cysteine biosynthesis/oxidative stress response, urease activity, and sortasedependent proteins. Metabolomic analysis showed that the luxS mutation affected cellular levels of fermentation products, fatty acids and amino acids. Cell density-dependent changes (log phase versus stationary phase growth) in gene transcription were not detected, indicating that AI-2 was unlikely to be involved in gene regulation mediated by quorum sensing in L. reuteri 100-23.

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Section: Resultssupporting

confidence: 64%

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

et al. 2012

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“…In contrast, the genes with predicted involvement in folate biosynthesis, encoding FolC (EC:6.3.2.12) and SulD (FolB; EC:4.1.2.25), were relatively more abundant in kittens fed the kibbled diet compared to the canned diet (FDR < 0.05; Table 5). Both folC , which encodes dihydrofolate/folylpolyglutamate synthase, and suID which encodes dihydroneopterin aldolase, are prevalent among Streptococcus and Lactobacillus genomes5960616263, and these taxa were significantly enriched in kittens fed the kibbled diet. The increased metagenome potential for synthesising thiamine and riboflavin in kittens fed the canned diet, but increased potential for folate synthesis in kibble-fed kittens, suggests scope for improving both types of diets to optimise microbial vitamin B biosynthesis in the cat.…”

Section: Resultsmentioning

confidence: 99%

Pre- and post-weaning diet alters the faecal metagenome in the cat with differences in vitamin and carbohydrate metabolism gene abundances

Young

Moon

Thomas

et al. 2016

Sci Rep

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Dietary format, and its role in pet nutrition, is of interest to pet food manufacturers and pet owners alike. The aim of the present study was to investigate the effects of pre- and post-weaning diets (kibbled or canned) on the composition and function of faecal microbiota in the domestic cat by shotgun metagenomic sequencing and gene taxonomic and functional assignment using MG-RAST. Post-weaning diet had a dramatic effect on community composition; 147 of the 195 bacterial species identified had significantly different mean relative abundances between kittens fed kibbled and canned diets. The kittens fed kibbled diets had relatively higher abundances of Lactobacillus (>100-fold), Bifidobacterium (>100-fold), and Collinsella (>9-fold) than kittens fed canned diets. There were relatively few differences in the predicted microbiome functions associated with the pre-weaning diet. Post-weaning diet affected the abundance of functional gene groups. Genes involved in vitamin biosynthesis, metabolism, and transport, were significantly enriched in the metagenomes of kittens fed the canned diet. The impact of post-weaning diet on the metagenome in terms of vitamin biosynthesis functions suggests that modulation of the microbiome function through diet may be an important avenue for improving the nutrition of companion animals.

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“…Growth was monitored in continuously stirred vessels with 400 ml regular MRS medium [26](for the CO 2 dependency experiments) or a chemically defined medium (in case of the acetate dependency experiments). The latter medium was described for Lactobacillus reuteri [27] and has previously been shown to also support growth of Lactobacillus johnsonii NCC 533. Batches were sparged with specific gas mixtures varying in CO 2 and O 2 content.…”

Section: Methodsmentioning

confidence: 99%

Oxygen Relieves the CO2 and Acetate Dependency of Lactobacillus johnsonii NCC 533

et al. 2013

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Oxygen relieves the CO2 and acetate dependency of Lactobacillus johnsonii NCC 533. The probiotic Lactobacillus johnsonii NCC 533 is relatively sensitive to oxidative stress; the presence of oxygen causes a lower biomass yield due to early growth stagnation. We show however that oxygen can also be beneficial to this organism as it relieves the requirement for acetate and CO2 during growth. Both on agar- and liquid-media, anaerobic growth of L. johnsonii NCC 533 requires CO2 supplementation of the gas phase. Switching off the CO2 supply induces growth arrest and cell death. The presence of molecular oxygen overcomes the CO2 dependency. Analogously, L. johnsonii NCC 533 strictly requires media with acetate to sustain anaerobic growth, although supplementation at a level that is 100-fold lower (120 microM) than the concentration in regular growth medium for lactobacilli already suffices for normal growth. Analogous to the CO2 requirement, oxygen supply relieves this acetate-dependency for growth. The L. johnsonii NCC 533 genome indicates that this organism lacks genes coding for pyruvate formate lyase (PFL) and pyruvate dehydrogenase (PDH), both CO2 and acetyl-CoA producing systems. Therefore, C1- and C2- compound production is predicted to largely depend on pyruvate oxidase activity (POX). This proposed role of POX in C2/C1-generation is corroborated by the observation that in a POX deficient mutant of L. johnsonii NCC 533, oxygen is not able to overcome acetate dependency nor does it relieve the CO2 dependency.

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Effect of Amino Acid Availability on Vitamin B ₁₂ Production in Lactobacillus reuteri

Cited by 31 publications

References 21 publications

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Pre- and post-weaning diet alters the faecal metagenome in the cat with differences in vitamin and carbohydrate metabolism gene abundances

Oxygen Relieves the CO2 and Acetate Dependency of Lactobacillus johnsonii NCC 533

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Effect of Amino Acid Availability on Vitamin B 12 Production in Lactobacillus reuteri

Cited by 31 publications

References 21 publications

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

Pre- and post-weaning diet alters the faecal metagenome in the cat with differences in vitamin and carbohydrate metabolism gene abundances

Oxygen Relieves the CO2 and Acetate Dependency of Lactobacillus johnsonii NCC 533

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Effect of Amino Acid Availability on Vitamin B ₁₂ Production in Lactobacillus reuteri