Effect of amino acids and antibodies against nerve growth factor receptors on the development of organotypic culture of lymphoid tissue

Abstract

We studied the effects of 20 L-amino acids on organotypic culture of splenic lymphoid tissue from 3-month-old rats were studied in the presence of apoptosis-inducing monoclonal antibodies against low-affinity receptors for nerve growth factor NGFRp75. The influence of amino acids stimulating cell proliferation in explants (lysine, asparagine, and glutamic acid) did not depend on NGFRp75. Hydrophobic amino acids inhibiting the growth zone in isolated application and abolished the inhibition of explant development in the presence of antibodies against NGFRp75. These amino acids can mediate the proapoptotic effect on lymphoid tissue via low-affinity receptors for nerve growth factor.

Effect of encoded amino acids on associative learning of honeybee Apis mellifera

“…In tissues of mesodermal origin, the proliferation occurs predominantly under effect of hydrophilic amino acids, whereas hydrophobic amino acids either produce the expressed proapoptotic effect or have no effect. In contrast to these phenomena, in tissues of the ectodermal origin, stimulation of proliferation occurs under effect of hydrophobic amino acids, while hydrophilic amino acids, except for histidine, have no stimulatory effect on development of the nervous tissue [4,5]. CHALISOVA et al tioned reflex background.…”

“…We used in this work all amino acids at a concentration of 10 -6 M that had turned out to be the most effective in our earlier pharmacological studies at the study of the capability for learning of honeybees [7] and at cultivation of mammalian tissues [4,5] (its effectiveness was revealed as a result of titration from 10 -3 M to 10 -12 M). It cannot be ruled out that some amino acids that have no stimulating effect can produce it at a change of concentration.…”