Effect of Amlodipine to MAP2 on Neurons Culture SH-SY5Y That Induced by Chronic Hyperglycemia

Abstract

Exposure to chronic hyperglycemia in neurons results in increased Ca2+ intracellular then causes increased oxidative stress. Oxidative stress will increase the activity of calcium activated neural protease causing MAP2 (microtubule associated protein 2) to decrease which will increase the cell death. The aim of research is to determine on the effects of amlodipine on the expression of MAP2 in SH-SY5Y neuron culture cells. This research is an experimental study, using SH-SY5Y neuron cell culture and then exposed to hyperglycemia for 6 days, then given amlodipine 10 nM and 100 nM. Then the MAP2 expression is seen. Based on the results of the Anova test on the administration of 10 nM, 100 nM and without amlodipine on the expression of MAP2 in culture of SH-SY5 neurons, which were 50 mM glucose for 6 days there was decrease in MAP2 (p = 0.082). Based on Spearman correlation test results, there was a strong relationship (p = 0.011) (r = -0.7791). There is decrease expression of the MAP2 after the administration of the dose of amlodipine 10 nM, 100 nM and without administration although not significant. It is estimated that another line activation occurs. One such pathway is the activation NMDA receptor will inhibit potential post synapse and increase calpain activation to cause a decrease in MAP2.