Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica

Guimarães, Bárbara O.; Gremmen, Pieter; Wijffels, René H.; Barbosa, María J.; D’Adamo, Sarah

doi:10.1016/j.aquaculture.2021.736526

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The higher DW level might be due to the presence of salt residues in microalgae after washing. This is supported by Guimarães et al [ 27 ] study, in which the first washing step only removed 93.8% Na and 69.4% K concentrations. Comparable AFDW values of the four microalgae obtained using three different washing cycles in the present study further supported the fact that one cycle is not enough to remove salt contents from marine microalgae.…”

Section: Discussionsupporting

confidence: 59%

“…The washing process is a requisite step in marine microalgal biomass determination as salts may present on microalgae surfaces or in water columns. Ammonium formate has been suggested to be one of the washing agents and widely utilized in removing the salts of marine microalgae [ 6 , 25 , 26 , 27 ]. The common concentration of ammonium formate that is used to clean the marine microalgae is 0.5 M ( Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

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A Recommendation for a Pre-Standardized Marine Microalgal Dry Weight Determination Protocol for Laboratory Scale Culture Using Ammonium Formate as a Washing Agent

Khaw

Tan

Sopawong

et al. 2021

Biology

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Microalgal biomass is one of the crucial criteria in microalgal studies. Many reported methods, even the well-established protocol on microalgal dry weight (DW) determination, vary greatly, and reliable comparative assessment amongst published results could be problematic. This study aimed to determine the best condition of critical parameters in marine microalgal DW determination for laboratory-scale culture using four different marine microalgal species. These parameters included the washing process, grades of glass microfiber filter (GMF), GMF pretreatment conditions, washing agent (ammonium formate) concentrations, culture: washing agent ratios (v:v) and washing cycles. GMF grade GF/A with precombustion at 450 °C provided the most satisfactory DW and the highest ash-free dry weight (AFDW)/DW ratio. Furthermore, 0.05 M ammonium formate with 1:2 culture: washing agent ratio and a minimum of two washing cycles appeared to be the best settings of microalgal DW determination. The present treatment increased the AFDW/DW ratio of the four respective microalgae by a minimum of 19%. The findings of this study could serve as a pivotal reference in developing a standardized protocol of marine microalgal DW determination to obtain veracious and reliable marine microalgal DW.

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Section: Discussionsupporting

confidence: 59%