Effect of amniotic fluid on the in vitro culture of human corneal endothelial cells

Feizi, Sepehr; Soheili, Zahra‐Soheila; Bagheri, Abouzar; Balagholi, Sahar; Mohammadian, Azam; Rezaeikanavi, Mozhgan; Ahmadieh, Hamid; Samiei, Shahram; Negahban, Kambiz

doi:10.1016/j.exer.2014.04.002

Cited by 21 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The intermediate filament vimentin was expressed much higher in the CSCs as opposed to the cell line, possibly demonstrating the absence of endothelial‐to‐mesenchymal transition in the B4G12 cells. However, presence of this protein has been demonstrated in histological sections of corneal endothelium in situ and in cultured HCEnCs as well …”

Section: Discussionmentioning

confidence: 53%

“…However, presence of this protein has been demonstrated in histological sections of corneal endothelium in situ and in cultured HCEnCs as well. 26,27 The expression levels of tight junction genes ZO-1 and claudin 14 were also confirmed in the cell line and the primary HCEnCs; interestingly, claudin 10 was not detectable by qPCR in the latter cells, which differed from the data obtained by immunofluorescent staining of the protein, and also, not in line with the findings of others. 28 Other groups have reported detecting claudin expression in B4G12 cells, which differed from that of primary human endothelium.…”

Section: Discussionmentioning

confidence: 74%

See 1 more Smart Citation

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells

Nagymihály

Veréb

Albert

et al. 2017

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 74%

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells

Nagymihály

Veréb

Albert

et al. 2017

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

show abstract

“…As such, it plays important roles in preserving corneal dehydration and transparency 51 . Vimentin is a major component of the mesenchymal cell cytoskeleton and is also expressed in human CECs 52 . Due to its neuroectodermal origin, the corneal endothelium represents a special case with respect to the expression of cadherin.…”

Section: Discussionmentioning

confidence: 99%

In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

Han

Lei

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Corneal endothelial cells (CECs) are very important for the maintenance of corneal transparency. However, in vitro, CECs display limited proliferation and loss of phenotype via endothelial to mesenchymal transformation (EMT) and cellular senescence. In this study, we demonstrate that continuous supplementary nutrition using a perfusion culture bioreactor and three-dimensional (3D) spheroid culture can be used to improve CEC expansion in culture and to construct a tissue-engineered CEC layer. Compared with static culture, perfusion-derived CECs exhibited an increased proliferative ability as well as formed close cell-cell contact junctions and numerous surface microvilli. We also demonstrated that the CEC spheroid culture significantly down-regulated gene expression of the proliferation marker Ki67 and EMT-related markers Vimentin and α-SMA, whereas the gene expression level of the CEC marker ATP1A1 was significantly up-regulated. Furthermore, use of the perfusion system in conjunction with a spheroid culture on decellularized corneal scaffolds and collagen sheets promoted the generation of CEC monolayers as well as neo-synthesized ECM formation. This study also confirmed that a CEC spheroid culture on a curved collagen sheet with controlled physiological intraocular pressure could generate a CEC monolayer. Thus, our results show that the use of a perfusion system and 3D spheroid culture can promote CEC expansion and the construction of tissue-engineered corneal endothelial layers in vitro.

show abstract

“…This showed that 20% HAFcontaining medium exhibited a greater stimulatory effect on HCEC growth and could represent a potential enriched supplement for HCEC regeneration studies [32].…”

Section: Human Amniotic Fluidmentioning

confidence: 99%

Concise Review: An Update on the Culture of Human Corneal Endothelial Cells for Transplantation

Parekh

Ferrari

Sheridan

et al. 2015

Stem Cells Translational Medicine

View full text Add to dashboard Cite

The cornea forms the front window of the eye, enabling the transmission of light to the retina through a crystalline lens. Many disorders of the cornea lead to partial or total blindness, and therefore corneal transplantation becomes mandatory. Recently, selective corneal layer (as opposed to full thickness) transplantation has become popular because this leads to earlier rehabilitation and visual outcomes. Corneal endothelial disorders are a common cause of corneal disease and transplantation. Corneal endothelial transplantation is successful but limited worldwide because of lower donor corneal supply. Alternatives to corneal tissue for endothelial transplantation therefore require immediate attention. The field of human corneal endothelial culture for transplantation is rapidly emerging as a possible viable option. This manuscript provides an update regarding these developments. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:258-264 SIGNIFICANCEThe cornea is the front clear window of the eye. It needs to be kept transparent for normal vision. It is formed of various layers of which the posterior layer (the endothelium) is responsible for the transparency of the cornea because it allows the transport of ions and solutes to and from the other layers of the cornea. Corneal blindness that results from the corneal endothelial dysfunction can be treated using healthy donor tissues. There is a huge demand for human donor corneas but limited supply, and therefore there is a need to identify alternatives that would reduce this demand. Research is underway to understand the isolation techniques for corneal endothelial cells, culturing these cells in the laboratory, and finding possible options to transplant these cells in the patients. This review article is an update on the recent developments in this field.

show abstract

Effect of amniotic fluid on the in vitro culture of human corneal endothelial cells

Cited by 21 publications

References 43 publications

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells

In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

Concise Review: An Update on the Culture of Human Corneal Endothelial Cells for Transplantation

Contact Info

Product

Resources

About