Effect of an Abnormal Sperm Chromatin Structural Assay (Scsa) on Pregnancy Outcome Following (Ivf) With Icsi in Previous Ivf Failures

Check, Jerome H.; Graziano, Vincent; Cohen, Rachael; Krotec, J.W.; Check, M. L.

doi:10.1080/014850190518125

Cited by 91 publications

(57 citation statements)

References 19 publications

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“…Several studies have failed to confirm these earlier findings by demonstrating no significant differences in pregnancy rates following IVF or ICSI between patients with high and low levels of sperm DNA damage [13,16,17,46,63,108,131]. Successful pregnancies have also been obtained using IVF or ICSI cycles despite high levels of sperm chromatin damage (DFI > 27%) [16,17,21,46]. A recent study found no significant differences in IVF and ICSI pregnancy outcomes between low and high DFI groups.…”

Section: In Vitro Fertilizationmentioning

confidence: 54%

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Schulte

Ohl

Sigman³

et al. 2009

J Assist Reprod Genet

227

159

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Male factor infertility is the sole cause of infertility in approximately 20% of infertile couples, with an additional 30% to 40% secondary to both male and female factors. Current means of evaluation of male factor infertility remains routine semen analysis including seminal volume, pH, sperm concentration, motility, and morphology. However, approximately 15% of patients with male factor infertility have a normal semen analysis and a definitive diagnosis of male infertility often cannot be made as a result of routine semen analysis. Attention has focused on the role of sperm nuclear DNA integrity in male factor infertility. Here we review the structure of human sperm chromatin, the etiology and mechanisms of sperm DNA damage, current tests available to assess sperm DNA integrity, and effect of sperm DNA integrity on reproductive outcomes.

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Section: In Vitro Fertilizationmentioning

confidence: 54%

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Schulte

Ohl

Sigman³

et al. 2009

J Assist Reprod Genet

227

159

View full text Add to dashboard Cite

show abstract

“…As with IVF studies, these ICSI studies are quite heterogeneous in terms of design, inclusion/exclusion criteria, and the sperm DNA damage test employed. In keeping with a recent analysis [Collins et al 2008], the results of this updated meta-analysis on ICSI studies indicate that sperm DNA damage is not related to ICSI pregnancy rates (combined OR of 1.15, 95% 0.90, 1.55, p = 0.65) [Benchaib et al 2007;Boe-Hansen et al 2006;Borini et al 2006;Bungum et al 2007;Check et al 2005;Gandini et al 2004;Hammadeh et al 1996;Henkel et al 2003;Host et al 2000;Huang et al 2005;Lin et al 2008;Micinski et al 2009;Tarozzi et al 2009;Zini et al 2005]. The characteristics of the 14 ICSI studies that could be evaluated are shown in Tables 4 and 5.…”

Section: In Vitro Fertilization With Intracytoplasmic Sperm Injectionmentioning

confidence: 81%

Are sperm chromatin and DNA defects relevant in the clinic?

Zini

2011

Systems Biology in Reproductive Medicine

228

196

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There has been an increase in the use of sperm DNA and chromatin integrity tests in the evaluation of the infertile man with the hypothesis that these tests may better diagnose infertility and predict reproductive outcomes. This review discusses the etiology of sperm DNA damage, briefly describing the tests of sperm DNA damage, and evaluates the relationship between sperm DNA damage and reproductive outcomes. A systematic review of the literature allows us to conclude that sperm DNA damage is associated with lower natural, intra-uterine insemination (IUI), and in vitro fertilization (IVF) pregnancy rates. Studies to date have not shown a clear association between sperm DNA and chromatin defects and pregnancy outcomes after intra-cytoplasmic sperm injection (ICSI). However, we cannot exclude the possibility that very high levels of DNA damage will impact on ICSI outcomes. In couples undergoing IVF or ICSI, there is evidence to show that sperm DNA damage is associated with an increased risk of pregnancy loss. A limitation of this systematic review and meta-analysis is that it does not address the heterogeneity of the individual study characteristics. Although the clinical utility of tests of sperm DNA damage remains to be firmly established, the data suggest that there is clinical value in testing couples prior to assisted reproductive technologies (ARTs IUI, IVF, and ICSI) and in those couples with recurrent miscarriages. Additional, well-designed prospective studies are needed before testing becomes a routine part of patient care.

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“…Recent study by Venkatesh et al, 2009 andLewis et al, 2010 [12, 13] showed that there is no correlation between oxidative stress (OS) or DNA damage with SSP. So there is a need to have specific diagnostic tests for the assessment of OS and DNA damage Studies both, in vitro and in vivo have shown that there is a negative correlation between sperm DNA integrity and fertility [14][15][16][17][18][19][20]. DNA damage is associated with poor embryo development, decreased implantation, and poor pregnancy outcome [21].…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

137

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Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented.

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Effect of an Abnormal Sperm Chromatin Structural Assay (Scsa) on Pregnancy Outcome Following (Ivf) With Icsi in Previous Ivf Failures

Cited by 91 publications

References 19 publications

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Are sperm chromatin and DNA defects relevant in the clinic?

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

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