Effect of an Antibacterial Polysaccharide Produced by Chaetomium globosum CGMCC 6882 on the Gut Microbiota of Mice

Abstract

Previously, a polysaccharide produced by Chaetomium globosum CGMCC 6882 was found to have antibacterial activity, but its toxic effects on body health and gut microbiota were concealed. Recent results showed that this polysaccharide was safe to Caco-2 cells and mice, while it reduced the body weight gain of mice from 10.5 ± 1.21 g to 8.4 ± 1.17 g after 28 days administration. Acetate, propionate, butyrate and total short-chain fatty acids concentrations increased from 23.85 ± 1.37 μmol/g, 10.23 ± 0.78 μmol/g, 7.15 ± 0.35 μmol/g and 41.23 ± 0.86 μmol/g to 42.77 ± 1.29 μmol/g, 20.03 ± 1.44 μmol/g, 12.06 ± 0.51 μmol/g and 74.86 ± 2.07 μmol/g, respectively. Furthermore, this polysaccharide enriched the abundance of gut microbiota and the Firmicutes/Bacteroidetes ratio was increased from 0.5172 to 0.7238. Overall, this study provides good guidance for the promising application of polysaccharides as preservatives in foods and in other fields in the future.

The Gut Mycobiome Characterization of Gestational Diabetes Mellitus and Its Association With Dietary Intervention

“…The most important finding was that the level of Ganoderma markedly increased in GDM-W2 samples after 2 weeks of diet intervention, suggesting the beneficial effect of diet on GDM patient management through its modulation of the compositions of some probiotic fungi. Another polysaccharide-producing fungus was Chaetomium , members of which were found to have antibacterial activity ( Sun et al, 2021b ). Anti-inflammatory polysaccharides from Chaetomium nigricolor were reported to inhibit nuclear factor-kappa B and c-Jun N-terminal kinase activation, in turn suppressing levels of proinflammatory mediators and cytokines ( Kim et al, 2020 ).…”

Effect of Polysaccharide Extracted From Gynostemma Pentaphyllum on the Body Weight and Gut Microbiota of Mice

“…Li et al ( 18 ) also found that Eucommia ulmoides polysaccharide was non-toxic to Raw 264.7 macrophages. In our previous works, we found that Ganoderma lucidum polysaccharide and Chaetomium globosum CGMCC 6882 exo-polysaccharide were not toxic to Caco-2 cells ( 13 , 14 ). In the present work, experimental mice showed no symptoms of death or disease, suggesting the safety of GPP to mice, which was in consistent with the safe result of GPP to Caco-2 cells.…”

“…Toxicity of GPP was determined using methyl thiazolyl tetrazolium (MTT) method against Caco-2 cells by using a previously reported method with some modifications ( 14 ). The freeze-dried GPP powder was dissolved and stirred in DMEM to concentrations of 50, 100, 200, 400, and 800 μg/ml, respectively.…”

“…The organic supernatant was collected and mixed with 1.0 g of anhydrous calciumchloride for 10 min to remove water. The supernatant was filtered against a 0.22 μm organic-based filter membrane, and SCFAs in organic layer were analyzed according to previously reported methods ( 14 ).…”

“…Gut microbiota was extracted and detected according to method reported previously with some modifications ( 14 ). Briefly, the total microbial DNA in the bacteria of collected cecum contents was extracted by using DNA extraction kit.…”