Effect of an antimicrobial drug on lung microbiota in healthy dogs

Fastrès, Aline; Taminiau, Bernard; Vangrinsven, Emilie; Tutunaru, Alexandru-Cosmin; Moyse, Evelyne; Farnir, Frédéric; Daube, Georges; Clercx, Cécile

doi:10.1016/j.heliyon.2019.e02802

Cited by 15 publications

(28 citation statements)

References 28 publications

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“…Bronchoscopy, bronchoalveolar lavage (BAL) procedure, and BALF processing and analysis were performed as already described [1,29]. Briefly, dogs were anesthetized using various protocols at the discretion of a board-certified anesthesiologist.…”

Section: Balf Collection and Processingmentioning

confidence: 99%

“…DNA was extracted from crude BALFs and PCSs previously banked at −80 °C, following the protocol provided with the DNeasy Blood and Tissue kit (QIAGEN Benelux BV; Antwerp, Belgium) as already described [29,34]. Total DNA quantity and purity were measured after extraction using the ND-1000 spectrophotometer (Nan-oDrop ND-1000, Isogen, De Meern, The Netherlands).…”

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

“…Duplicate qPCRs targeting the V2-V3 region of the 16S rDNA were performed to evaluate the bacterial load in the lung as already described [29,35]. qPCRs were conducted in a final volume of 20 µL containing 2.5 μL of template DNA, 0.5 μL of forward primer (5′-ACT CCT ACG GGA GGC AGC AG-3′; 0.5 μM), 0.5 μL of reverse primer (5′-ATT ACC GCG GCT GCTGG-3′; 0.5 μM), 10 μL of No Rox SYBR 2 × MasterMix (Eurogentec, Seraing, Belgium), and 6.5 μL of water.…”

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

“…To characterize the bacterial populations in samples, the V1-V3 region of the bacterial 16S rDNA gene was amplified using the forward primer (5′-GAG AGT TTG ATY MTGG CTC AG-3′) and the reverse primer (5′-ACC GCG GCT GCT GGCAC-3′) with Illumina overhand adapters as previously described [29,34]. PCRs were conducted and amplicons obtained purified with the Agencourt AMPure XP beads kit (Beckman Coulter, Villepinte, France), indexed using the Nextera XT index primers 1 and 2 and quantified by PicoGreen (Ther-moFisher Scientific, Waltham, MA, USA) before normalization and pooling to form libraries.…”

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

“…Studies in acute pneumonia indicate that the 16S rDNA sequencing improves the microbiological yield and could help to guide antimicrobial therapy [20,27]. In dogs, the lung microbiota (LM) has only been studied in bacterial secondary or community-acquired pneumonia (CAP) and only few data are available in experimental healthy beagles [28][29][30] and healthy dogs from other breeds [31]. In dogs with pneumonia, a dysbiosis of the LM was observed with the loss of bacteria found in health and the domination, mostly in CAP, of one or two bacteria [30].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction

Fastrès

Taminiau

et al. 2020

Vet Res

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Infection with Bordetella bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc. In diseased compared with healthy dogs, the β-diversity changed (P = 0.0024); bacterial richness and α-diversity were lower (P = 0.012 and 0.0061), and bacterial load higher (P = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P = 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases. © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Section: Balf Collection and Processingmentioning

confidence: 99%

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

Section: S Rdna Amplicon Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction

Fastrès

Taminiau

et al. 2020

Vet Res

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Bacterial Respiratory Infections (Tracheobronchitis, Pneumonia, and Pyothorax)

Reinero¹,

Lee‐Fowler²

2021

Greene's Infectious Diseases of the Dog and Cat

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Individual differences in behaviour and gut bacteria are associated in collared peccary (Mammalia, Tayassuidae)

et al. 2021

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Aims: We tested the hypothesis that the behaviour of an individual is associated with the diversity of its gut bacteria, using the collared peccary () as a model. Pecari tajacu Methods and Results: In all, 24 adult male collared peccaries received either low-( 12) or high-fibre diet ( 12) to induce contrasting gut n = n = fermentation profiles. They were submitted to three short-term challenges, allowing us to rate the animals in a coping-style dimension named 'calmness'. At the end of the experimental period, we collected samples of peccaries' forestomach contents to characterize bacterial diversity. We found a significant positive association between individual 'calmness' z-scores and the bacterial evenness index in gut bacteria (and a similar trend with the Simpson's diversity index), suggesting a more homogeneous bacterial community of calmer individuals. We also found a positive association between fibres digestibility and gut bacterial diversity in the peccaries' forestomach, but no effect of the dietary fibre level. Conclusions: Gut bacteria evenness increases with 'calmness' z-scores, suggesting a more homogeneous bacterial community of calmer individuals, compared with the more heterogeneous of the most distressed ones. Our results also suggest associations between the digestibility of ADF with the gut bacterial diversity indices and with the relative abundance of the Actinobacteria phylum. Significance and Impact of the Study: Our data showed that the hosts' individual behavioural differences are potentially aligned with gut bacterial diversity. The behaviour-microbiota link is correlated with host feed efficiency and, ultimately, may have implications for animal health and welfare of farm animals.

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Effect of an antimicrobial drug on lung microbiota in healthy dogs

Cited by 15 publications

References 28 publications

Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction

Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction

Bacterial Respiratory Infections (Tracheobronchitis, Pneumonia, and Pyothorax)

Individual differences in behaviour and gut bacteria are associated in collared peccary (Mammalia, Tayassuidae)

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