Effect of an ecdysone agonist, RH-5849, on wandering behaviour in Spodoptera littoralis

Pszczółkowski, Maciej A.; Kuszczak, Barbara

doi:10.1016/0742-8413(95)02064-0

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…Larvae of S. littoralis were reared under L16:D8 at 24 • C, on a modified semiartificial diet prescribed by Shorey & Hale (1965), and modified by Pszczolkowski & Kuszczak (1996). Larvae of S. littoralis were reared under L16:D8 at 24 • C, on a modified semiartificial diet prescribed by Shorey & Hale (1965), and modified by Pszczolkowski & Kuszczak (1996).…”

Section: Methodsmentioning

confidence: 99%

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Endocrine background of how 20‐hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in Spodoptera littoralis

Pszczółkowski

Kuszczak

Smagghe

1998

Entomologia Exp Applicata

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Under laboratory conditions (L16:D8, 24 • C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2-dibenzoyl-1-tert-butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose-dependent. In addition, non-lethal doses of RH 5849, ranging from 0.05 to 10 µg/larva were used. Following treatment of S. littoralis larvae with 5 µg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non-steroidal ecdysone agonists should consider the proper circadian timing of application in the field.

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Section: Methodsmentioning

confidence: 99%

“…Insects. Larvae of S. littoralis were reared under L16:D8 at 24 • C, on a modified semiartificial diet prescribed by Shorey & Hale (1965), and modified by Pszczolkowski & Kuszczak (1996). They were selected from the stock colony as young penultimate instar larvae and stored in transparent plastic boxes until they reached the final instar.…”

Section: Methodsmentioning

confidence: 99%

Endocrine background of how 20‐hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in Spodoptera littoralis

Pszczółkowski

Kuszczak

Smagghe

1998

Entomologia Exp Applicata

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“…The 5th (penultimate) instar larvae prepared for molting (identified by head capsule slippage) were separated from the stock culture and observed hourly for the selection of freshly molted individuals of the 6th instar. Only second gate larvae were collected and used (see [ 48 ] for details); in specific experiments, freshly molted larvae were transferred into constant light (LL). The experiments were done on 2-day-old larvae of the 6th instar.…”

Section: Methodsmentioning

confidence: 99%

Molecular Oscillator Affects Susceptibility of Caterpillars to Insecticides: Studies on the Egyptian Cotton Leaf Worm—Spodoptera littoralis (Lepidoptera: Noctuidae)

Darwich

Chrzanowski

Bernatowicz

et al. 2022

Insects

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The molecular oscillator is the core of the biological clock and is formed by genes and proteins whose cyclic expression is regulated in the transcriptional-translational feedback loops (TTFLs). Proteins of the TTFLs are regulators of both their own and executive genes involved in the control of many processes in insects (e.g., rhythmic metabolism of xenobiotics, including insecticides). We disrupted the clock operation in S. littoralis larvae by injecting the dsRNA of clock genes into their body cavity and culturing the larvae under continuous light. As a result, the daily susceptibility of larvae to insecticides was abolished and the susceptibility itself increased (in most cases). In the fat body, midgut, and Malpighian tubules (the main organs metabolizing xenobiotics) of the larvae treated with injected-dsRNA, the daily activity profiles of enzymes involved in detoxification—cytochrome P450 monooxygenases, Glutathione-S-transferase, and esterase—have changed significantly. The presented results prove the role of the molecular oscillator in the regulation of larvae responses to insecticides and provide grounds for rational use of these compounds (at suitable times of the day), and may indicate clock genes as potential targets of molecular manipulation to produce plant protection compounds based on the RNAi method.

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Effect of an ecdysone agonist, RH-5849, on wandering behaviour in Spodoptera littoralis

Cited by 2 publications

References 27 publications

Endocrine background of how 20‐hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in Spodoptera littoralis

Endocrine background of how 20‐hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in Spodoptera littoralis

Molecular Oscillator Affects Susceptibility of Caterpillars to Insecticides: Studies on the Egyptian Cotton Leaf Worm—Spodoptera littoralis (Lepidoptera: Noctuidae)

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