Effect of an electric field on a floating lipid bilayer: A neutron reflectivity study

Lecuyer, Sigolène; Fragneto, Giovanna; Charitat, Thierry

doi:10.1140/epje/i2006-10054-8

Cited by 38 publications

(38 citation statements)

References 21 publications

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“…Charitat and colleagues have performed high resolution neutron studies of floating bilayers, which are exposed to electric potentials in aqueous environments. They have obtained no thickness changes even for strong, alternating electric fields (10 9 V/m, 10 Hz) [LFC06,CLF08]. These results were consistent with reference experiments using solely single bilayers, which were bound to the substrate.…”

Section: Electric Field Effect On Membrane Structuresupporting

confidence: 85%

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Black lipid membranes studied by x-ray phase contrast imaging

Beerlink¹

2011

Göttingen Series in X-Ray Physics

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Section: Electric Field Effect On Membrane Structuresupporting

confidence: 85%

“…These variations in the layer structure follow the predictions of Burgess [LFC06,CLF08]. The influences of the supporting substrate remain unclear and need to be investigated in future experiments.…”

Section: Discussionsupporting

confidence: 62%

Black lipid membranes studied by x-ray phase contrast imaging

Beerlink¹

2011

Göttingen Series in X-Ray Physics

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“…These stresses act to increase the membrane area, thus producing a negative electrostatic correction to the membrane tension. This contribution has been recognized to drive instabilities in membranes when a normal DC electric field is applied [6,39,45].…”

Section: Interpretation Of the Electrostatic Contribution To The Surfmentioning

confidence: 99%

Electrostatic and electrokinetic contributions to the elastic moduli of a driven membrane

et al. 2009

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Abstract. We discuss the electrostatic contribution to the elastic moduli of a cell or artificial membrane placed in an electrolyte and driven by a DC electric field. The field drives ion currents across the membrane, through specific channels, pumps or natural pores. In steady state, charges accumulate in the Debye layers close to the membrane, modifying the membrane elastic moduli. We first study a model of a membrane of zero thickness, later generalizing this treatment to allow for a finite thickness and finite dielectric constant. Our results clarify and extend the results presented in [D. Lacoste, M. Cosentino Lagomarsino, and J. F. Joanny, Europhys. Lett., 77, 18006 (2007)], by providing a physical explanation for a destabilizing term proportional to k 3 ⊥ in the fluctuation spectrum, which we relate to a nonlinear (E 2 ) electro-kinetic effect called induced-charge electro-osmosis (ICEO). Recent studies of ICEO have focused on electrodes and polarizable particles, where an applied bulk field is perturbed by capacitive charging of the double layer and drives flow along the field axis toward surface protrusions; in contrast, we predict "reverse" ICEO flows around driven membranes, due to curvature-induced tangential fields within a non-equilibrium double layer, which hydrodynamically enhance protrusions. We also consider the effect of incorporating the dynamics of a spatially dependent concentration field for the ion channels.

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“…73 The main result of this work was the observation of the complete unbinding of the floating bilayer, by application of an electric field on a double DSPC bilayer in a CaCl 2 solution. The effect occurs at low frequency ͑10 Hz͒ and for a voltage amplitude higher than 5 V. Figure 18 shows reflectivity data before and after applying the electric field.…”

Section: B Destabilization By An Electric Fieldmentioning

confidence: 99%

Fluctuations and destabilization of single phospholipid bilayers

2008

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Supported phospholipid bilayers are interesting model systems for biologists and present fascinating physical properties. The authors present an extensive experimental study of the dynamic properties of supported bilayers. The structure and the equilibrium properties of single and double supported bilayers were investigated with neutron reflectivity. The submicronic fluctuation spectrum of a nearly free "floating" bilayer was determined using off-specular x-ray scattering: the surface tension of the bilayer, its bending modulus, and the intermembrane potential could be determined. Using fluorescence microscopy, the authors showed that this well-controlled single bilayer can form vesicles. Destabilization occurred either at the main gel-fluid transition of the lipids and could be interpreted in terms of a decrease in the bending rigidity or under an ac low-frequency electric field applied in the fluid phase. In the latter case, the authors also studied the effect of the electric field at the molecular length scale by neutron reflectivity. In both cases, destabilization leads to the formation of relatively monodisperse vesicles. This could give further understanding on the vesicle formation mechanism and on the parameters that determine the vesicle size.

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Effect of an electric field on a floating lipid bilayer: A neutron reflectivity study

Cited by 38 publications

References 21 publications

Black lipid membranes studied by x-ray phase contrast imaging

Black lipid membranes studied by x-ray phase contrast imaging

Electrostatic and electrokinetic contributions to the elastic moduli of a driven membrane

Fluctuations and destabilization of single phospholipid bilayers

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