Effect of an Escherichia coli traD (ts) Mutation on MS2 RNA Replication

A strain variation of varicella-zoster virus that maps to the UL region of the genome was found to be due to different copy numbers of a high GC 42-base-pair repeat. DNA sequence analysis of this variable region showed the sequence to be 5-GCGGGATCGGGCTTTCGGG(A/T)AGCGGCCGAGGTGGGCGCGACG-3. Strains Scott and Webster both contain 7 and 32 copies of the repeat, whereas strain Oka has exactly 4 copies less. Microheterogeneity exists within the repeated sequences, depending on the strain and the repeat number. MATERIALS AND METHODS Cells and viruses. VZV strains Oka, Webster, and Ellen were obtained from the American Type Culture Collection. Strain Scott was a gift from G. Fischer, Department of Pediatrics, Uniformed Services University of the Health Sciences. All viruses were grown in human foreskin fibroblast cells as previously described (30, 34). DNAs. VZV DNA fragments generated by EcoRI and BamHI were cloned into pBR325 or GT.wesB as previously described (29, 33) or into pUC9 (39). Clones containing EcoRI-P or BamHI-F were identified for further analysis after colony hybridization. DNA fragments were separated by electrophoresis in 1 or 1.5% agarose gels (SeaKem LE type; FMC Corp., Marine Div., Rockland, Maine) run at 40 mA for 18 to 24 h in 0.1 M Tris borate-0.01 M EDTA (pH 660

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Effect of an Escherichia coli traD (ts) Mutation on MS2 RNA Replication

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Conjugative Pili and Pilus-Specific Phages

Conjugative Pili and Pilus-Specific Phages

Putative glycoprotein gene of varicella-zoster virus with variable copy numbers of a 42-base-pair repeat sequence has homology to herpes simplex virus glycoprotein C

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