Effect of an Intrauterine Device on the Gene Expression Profile of the Endometrium

Horcajadas, José A.; Sharkey, Andrew; Catalano, Rob D.; Sherwin, Joseph R.; Domı́nguez, Francisco; Burgos, L.; Castro, A. de; Peraza, M. R.; Pellicer, A.; Simón, Carlos

doi:10.1210/jc.2006-0430

Cited by 65 publications

(37 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, changes in gene expression are not necessarily reflected in changes in translated proteins, nor does gene analysis take into account posttranscriptional, translational, or posttranslational changes that relate to cyclical transitions. Of the hundreds of gene expression changes typically identified by microarray, relatively few are common to more than two studies [47,48]. Comparison of proteomic data with published gene expression data in similar cohorts of women [47,49,50] also have revealed an overall lack of correlation between the two, suggesting that posttranscriptional or translational regulation is an important feature in human biology.…”

Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Methods Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negativepregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. Results There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the nonblastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. Conclusions CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.

show abstract

Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Obihiro University (no. [18][19][20][21][22][23][24][25]. Twelve Holstein cows (3-7 yr of age) that had been ovariectomized 40 -50 days before the beginning of the experiment were maintained in a free-stall barn throughout the experimental period and had free access to food and water.…”

Section: Methodsmentioning

confidence: 99%

“…Changes in mRNA expression profiles in bovine endometrium during the estrous cycle were already evaluated in two studies (7,36). Microarray studies that give an insight into the changes occurring in gene expression in the endometrium during the sexual cycle have been performed also in humans (19), mice (26), sheep (12), and pigs (44). In cattle, two studies (8,28) have taken two different animal model approaches to address the differences in endometrial gene expression between pregnant and cycling animals on day 18.…”

mentioning

confidence: 99%

Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium

Shimizu

Krebs

Bauersachs

et al. 2010

Physiological Genomics

View full text Add to dashboard Cite

The aim of our study was to analyze endometrial gene expression profiles in ovariectomized cows treated with estradiol and/or progesterone by using microarray analysis. Clustering of differentially expressed genes allowed separation into distinct hormone response patterns. These patterns could be classified into independent and interdependent actions of the steroid hormones estrogen and progesterone. The use of ovariectomized cows and external administration of hormones identified a set of genes whose regulation depends on a progesterone priming effect. The progesterone-primed estrogen response comprises gene functions such as migration, cell differentiation, and cell adhesion and therefore may play a crucial role in tissue remodeling, as one of its key regulators in the endometrium, TGFB2, is among this group of progesterone-primed genes. Functional annotation analysis of the estrogen-responsive gene clusters shows a clear dominance of functions such as cell cycle, morphogenesis, and differentiation. The functional profile of the progesterone-responsive clusters is less clear but nevertheless shows some important fertility-related terms like luteinization, oocyte maturation, and catecholamine metabolism. We looked for putative regulators of the steroid hormone response in endometrium by searching for enriched transcription factor binding sites in the promoter regions of the genes with similar hormone response profile. This analysis identified transcription factors such as SP1, NFYA, FOXA2, IRF2, ESR1, and NOBOX as candidate regulators of gene expression in bovine endometrium treated with steroid hormones. Taken together, our data provide novel insights into the regulation of bovine endometrial physiology by steroid hormones.

show abstract

“…In the present study, the presence of the IUD had no significant effect on LIF expression in the endometrium. Horcajadas et al [19] investigated the expression of 147 genes in the endometrium of five females prior to IUD insertion, with the IUD in place and after IUD removal. The study found a significant decline in LIF expression with an IUD in place and 2 months after IUD removal compared to expression prior to IUD insertion.…”

Section: Discussionmentioning

confidence: 99%

Do Leukaemia Inhibitory Factor and Vascular Endothelial Growth Factor Have Any Roles in Intrauterine Device Mechanism of Action? An Experimental Rat Study

Çakmak

Ozalp²,

Açıkalın³

et al. 2015

Gynecol Obstet Invest

View full text Add to dashboard Cite

Objective: To assess leukaemia inhibitory factor (LIF) and vascular endothelial growth factor (VEGF) expression as markers of intrauterine device (IUD) efficacy in a rat model. Methods: Twenty nulliparous female Wistar rats were divided into two groups with 10 animals per group: group I (IUD) and group II (control group, no IUD). In group I, a 2-cm 3-0 silk suture was placed into one horn of the rat bicornuate uterus. On day 20 (after IUD insertion) rats were sacrificed and their uteri removed. The number of vessels and the distribution of LIF and VEGF were compared among the uterine horns. Results: There were no significant differences in LIF and VEGF expression in the groups and all horns (p > 0.05). The number of vessels was higher in the IUD+ horn than in the IUD- horn of group I and in the horn of group II (p < 0.05). There was no significant difference in the number of vessels between the IUD- horns of groups I and II (p > 0.05). Conclusion: LIF and VEGF expression did not correlate with IUD efficacy in a rat model. An IUD may increase the number of vessels in the uterine horn independent of VEGF expression.

show abstract

Effect of an Intrauterine Device on the Gene Expression Profile of the Endometrium

Abstract: Our results reveal that a refractory endometrium in a fertile woman produced by an IUD is induced by preventing the normal transition to a receptive gene expression profile through effects on a specific subset or cluster of genes that impact on endometrial receptivity.

Cited by 65 publications

References 27 publications

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium

Do Leukaemia Inhibitory Factor and Vascular Endothelial Growth Factor Have Any Roles in Intrauterine Device Mechanism of Action? An Experimental Rat Study

Contact Info

Product

Resources

About