Effect of angiotensin II and angiotensin‐(1–7) on proliferation of stem cells from human dental apical papilla

Abstract

The effects of the renin-angiotensin system (RAS) on stem cells isolated from human dental apical papilla (SCAPs) are completely unknown. Therefore, the aim of this study was to identify RAS components expressed in SCAPs and the effects of angiotensin (Ang) II and Ang-(1-7) on cell proliferation. SCAPs were collected from third molar teeth of adolescents and maintained in cell culture. Messenger RNA expression and protein levels of angiotensin-converting enzyme (ACE), ACE2, and Mas, Ang II type I (AT1) and type II (AT2) receptors were detected in SCAPs. Treatment with either Ang II or Ang-(1-7) increased the proliferation of SCAPs. These effects were inhibited by PD123319, an AT2 antagonist. While Ang II augmented mTOR phosphorylation, Ang-(1-7) induced ERK1/2 phosphorylation. In conclusion, SCAPs produce the main RAS components and both Ang II and Ang-(1-7) treatments induced cell proliferation mediated by AT2 activation through different intracellular mechanisms.

Unraveling the Role of the Apical Papilla During Dental Root Maturation

“…Analysis of the SCAP secretome in conditioned medium revealed a diverse collection of proteins consisting of chemokines along with angiogenic, immunomodulatory, anti-apoptotic, neuroprotective factors, and extracellular matrix (ECM) proteins ( Yu et al, 2020 ). Moreover, SCAP produce components of the renin-angiotensin system (RAS) ( Macedo et al, 2021 ) which could increase SCAP proliferation rate via angiotensin 2 receptor activation ( Pizzatto et al, 2020 ). Several studies indicate the capacity of the apical papilla to maintain cells in a non-differentiated state intrinsically and even in neighboring tissues via currently unknown secretory signaling molecules.…”