Effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of Anchusa italica

Azizi, Susan; Mohamad, Rosfarizan; Bahadoran, Azadeh; Bayat, Saadi; Rahim, Raha Abdul; Ariff, Arbakariya; Saad, Wan Zuhainis

doi:10.1016/j.jphotobiol.2016.06.007

Cited by 137 publications

(43 citation statements)

References 40 publications

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“…33 A small difference in the alloy nanoparticle size may be attributed to a minute variation in the annealing temperature. 34 However, since there is no difference between the nanoparticles synthesized in this study and those in our previous study with respect to their composition, these nanoparticles were considered apt for further experiments. The estimated volume of the particles was 21.1 nm 3 .…”

Section: Results and Discussion Characterization Of Nfasmentioning

confidence: 91%

Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Dhawan

et al. 2018

IJN

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IntroductionThe efficacy of a chemotherapy drug in cancer therapy is highly determined by the ability to control the rate and extent of its release in vivo. However, the lack of techniques to accurately control drug release drastically limits the potency of a chemotherapy drug.Materials and methodsHere, we present a novel strategy to precisely monitor drug release under magnetic stimulation. Methotrexate (MTX), an anticancer drug, was covalently functionalized onto iron–gold alloy magnetic nanoparticles (Fe–Au alloy nanoparticles or NFAs) through 2-aminoethanethiol grafting and the ability of this drug–nanoparticle conjugate (NFA–MTX) in limiting HepG2 (liver carcinoma) cell growth was studied. Well-dispersed NFAs were prepared through pyrolysis.Results and discussionTransmission electron microscopy revealed the average nanoparticle size to be 7.22±2.6 nm, while X-ray diffraction showed distinct 2θ peaks for iron and gold, confirming the presence of iron and gold nanoparticles. Inductively coupled plasma mass spectrometry revealed that the amount of NFA–MTX conjugate ingested by HepG2 cancer cells was 1.5 times higher than that ingested by L929 fibroblasts, thereby proving a higher selective ingestion by cancer cells compared to normal cells. Fourier-transform infrared spectroscopy revealed the breakage of Au-S bonds by the heat generated under magnetic field stimulation to release MTX from the NFA–MTX conjugate, triggering a 95% decrease in cellular viability at 100 µg/mL.ConclusionThe ability of NFA–MTX to dissociate under the influence of an applied magnetic field provides a new strategy to induce cancer cell death via hyperthermia. Applications in drug delivery, drug development, and cancer research are expected.

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Section: Results and Discussion Characterization Of Nfasmentioning

confidence: 91%

Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Dhawan

et al. 2018

IJN

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“…Peak at 3431 cm À1 might also originate from bonded eNH and eOH vibrations of eCOOH groups found in the P. granatum peel powder (Fig. 6a) [38]. The major absorption peaks found in P. granatum peel powder can be observed between 1000 cm À1 and 1750 cm À1 (Fig.…”

Section: Fourier Transform Infrared Spectroscopy Studymentioning

confidence: 87%

“…From literature reviews, it has been agreed that particle size is considered one of the main factors that affect the sensitivity of bacteria towards nanomaterials [14]. Based on several research papers, ZnO-NPs of particle sizes less than 37 nm were successfully synthesized in temperatures lower than 500 C [38]. MIC value for these ZnO-NPs against E. coli and E. faecalis were reported to be 32 mg/mL and 16 mg/mL, respectively [42].…”

Section: Evaluation Of Antibacterial Effects Of P Granatum/zno-npsmentioning

confidence: 99%

Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract

Sukri

Shameli

Wong

et al. 2019

Journal of Molecular Structure

179

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The unique properties of zinc oxide nanoparticles (ZnO-NPs) produced using plant extract make them attractive for use in medical as well as industrial applications, and it is necessary to develop environmentally friendly methods for their synthesis. This can be accomplished by replacing the traditional chemical compounds for the reduction of the zinc ions to ZnO-NPs during synthesis with natural plant extracts. Here, the biosynthesis of ZnO-NPs using Punica granatum (P. granatum) fruit peels extract was investigated as the reducing and stabilizing agent. The P. granatum/ZnO-NPs with spherical and hexagonal shapes were biosynthesized at different annealing temperatures. The X-ray diffraction analysis confirmed the synthesis of highly pure ZnO-NPs with increasing crystallinity in higher annealing temperatures. The ZnO-NPs displayed characteristic absorption peaks between 370 and 378 nm in the UV evis spectra. Transmission electron microscopy (TEM) imaging showed the formation of mostly spherical and hexagonal-shaped ZnO-NPs in the mean size of 32.98 nm and 81.84 nm at 600 C and 700 C respectively. According to FTIR spectrum, strong absorption bands in the range of 462e487 cm À1 corresponding to ZneO bond stretching can be seen. Antibacterial activities of P. granatum/ZnO-NPs against Escherichia coli (E. coli) and Enterococcus faecalis (E. faecalis) were investigated and compared. Results obtained show that smaller-sized P. granatum/ZnO-NPs are more effective in inhibiting growth of both bacteria. In addition, cytotoxicity assays were performed for P. granatum/ZnO-NPs against human colon normal and cancerous cells. P. granatum/ZnO-NPs exhibited similar killing activities of both cell lines at the concentration of !31.25 mg/mL. The biosynthesized ZnO-NPs could offer potential applications in biomedical field.

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“…In addition, the plant metabolites with pharmaceutical effects have the potential to be introduced on the nanoparticle surfaces during the synthesis procedure, which eventually leads to the occurrence of subsequent different surface effects in their medical applications. 16 Considering the advantages of biologic synthesis of metallic nanoparticles, we developed a green approach to synthesize Ag-NPs with fruit extract of Citrullus colocynthis (C. colocynthis).…”

Section: Introductionmentioning

confidence: 99%

In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases

Moniri¹,

Moghaddam²,

Azizi³

et al. 2018

IJN

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BackgroundIn recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression.ObjectivesThe aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties. The in vitro molecular study was performed to evaluate expression of genes involved in healing of wounds after treatment with BNC/Ag biofilms.Study design, materials, and methodsSilver nanoparticles were formed by using Citrullus colocynthis extract within new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Besides, swelling property and Ag release profile of the nanocomposites were studied. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was studied. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR.ResultsSpherical silver nanoparticles with particle size ranging from 20 to 50 nm were synthesized and impregnated within the structure of BNC. The resulting nanocomposites showed significant antibacterial activities with inhibition zones ranging from 7±0.25 to 16.24±0.09 mm against skin pathogenic bacteria. Moreover, it was compatible with human fibroblast cells (HDF) and could promote in vitro wound healing after 48h. Based on bioinformatics databases, the genes of TGF-β1, MMP2, MMP9, CTNNB1, Wnt4, hsa-miR-29b-3p and hsa-miR-29c-3p played important role in wound healing. The nanocomposites had an effect in expression of the genes in healing. Thus, the BNC/Ag nanocomposite can be used to heal wound in a short period and simple manner.ConclusionThis eco-friendly nanocomposite with excellent antibacterial activities and healing property confirming its utility as potential wound dressings.

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Effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of Anchusa italica

Cited by 137 publications

References 40 publications

Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract

In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases

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Effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of Anchusa italica

Cited by 137 publications

References 40 publications

Iron&ndash;gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Iron&ndash;gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract

In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases

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Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy