Effect of anti-fungal compound phytosphingosine in wheat root exudates on the rhizosphere soil microbial community of watermelon

Li, Chunxia; Tian, Qing; Rahman, Muhammad Khashi u; Wu, Fengzhi

doi:10.1007/s11104-020-04702-1

Cited by 42 publications

(27 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This activity was shown for several LCB species, including those LCBs mainly present in plants, phytosphingosine (t18:0) and dihydrosphingosine (d18:0) 19 . To our knowledge, antimicrobial effects of LCBs against plant pathogens received attention only recently 20 , and have not been systematically assessed. Therefore, we aimed to test the antimicrobial effect of phytosphingosine on those bacterial and fungal species interacting with plants.…”

Section: Introductionmentioning

confidence: 99%

The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens

Glenz

Kaiping

Göpfert

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum, Fusarium graminearum and Sclerotinia sclerotiorum, the plant symbiotic fungal endophyte Serendipita indica, the bacterial pathogens Pseudomonas syringae pv. tomato (Pst), Agrobacterium tumefaciens, and the related beneficial strain Rhizobium radiobacter. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 μg/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.

show abstract

Section: Introductionmentioning

confidence: 99%

The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens

Glenz

Kaiping

Göpfert

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In plants, it has been reported that phytosphingosine participates in plant stress signaling through crosstalk-regulated events [ 94 ], and it has been also found that this compound obtained from wheat root exudates displays anti-fungal activity on the rhizosphere microbial community associated with watermelon [ 95 ]. In this work, phytosphingosine was more abundant in red fruits than in green ones, indicating that this and other sphingolipids could play a role in the ripening process.…”

Section: Resultsmentioning

confidence: 99%

“…Phytosphingosine has been reported in microorganisms, yeasts, plants, fungi, and mammal cells as an essential component of the membrane, playing key roles in cell growth, survival and cell death, and mediating the response against stress as secondary messengers [90][91][92][93]. In plants, it has been reported that phytosphingosine participates in plant stress signaling through crosstalk-regulated events [94], and it has been also found that this compound obtained from wheat root exudates displays anti-fungal activity on the rhizosphere microbial community associated with watermelon [95]. In this work, phytosphingosine was more abundant in red fruits than in green ones, indicating that this and other sphingolipids could play a role in the ripening process.…”

Section: Phytosphingosinementioning

confidence: 99%

Identification of Compounds with Potential Therapeutic Uses from Sweet Pepper (Capsicum annuum L.) Fruits and Their Modulation by Nitric Oxide (NO)

Guevara

Domínguez-Anaya

Ortigosa

et al. 2021

IJMS

View full text Add to dashboard Cite

Plant species are precursors of a wide variety of secondary metabolites that, besides being useful for themselves, can also be used by humans for their consumption and economic benefit. Pepper (Capsicum annuum L.) fruit is not only a common food and spice source, it also stands out for containing high amounts of antioxidants (such as vitamins C and A), polyphenols and capsaicinoids. Particular attention has been paid to capsaicin, whose anti-inflammatory, antiproliferative and analgesic activities have been reported in the literature. Due to the potential interest in pepper metabolites for human use, in this project, we carried out an investigation to identify new bioactive compounds of this crop. To achieve this, we applied a metabolomic approach, using an HPLC (high-performance liquid chromatography) separative technique coupled to metabolite identification by high resolution mass spectrometry (HRMS). After chromatographic analysis and data processing against metabolic databases, 12 differential bioactive compounds were identified in sweet pepper fruits, including quercetin and its derivatives, L-tryptophan, phytosphingosin, FAD, gingerglycolipid A, tetrahydropentoxylin, blumenol C glucoside, colnelenic acid and capsoside A. The abundance of these metabolites varied depending on the ripening stage of the fruits, either immature green or ripe red. We also studied the variation of these 12 metabolites upon treatment with exogenous nitric oxide (NO), a free radical gas involved in a good number of physiological processes in higher plants such as germination, growth, flowering, senescence, and fruit ripening, among others. Overall, it was found that the content of the analyzed metabolites depended on the ripening stage and on the presence of NO. The metabolic pattern followed by quercetin and its derivatives, as a consequence of the ripening stage and NO treatment, was also corroborated by transcriptomic analysis of genes involved in the synthesis of these compounds. This opens new research perspectives on the pepper fruit’s bioactive compounds with nutraceutical potentiality, where biotechnological strategies can be applied for optimizing the level of these beneficial compounds.

show abstract

“…A range of chemical compounds have been found in root exudates that have been reported to act as repellents or chemoattractants of soil microbes, during both pathogenic and beneficial interactions. Recently, Li et al [ 31 ] reported the inhibitory activity of wheat root exudates against the pathogenic fungus F. oxysporum f. sp. niveum .…”

Section: Resultsmentioning

confidence: 99%

Plant Roots Release Small Extracellular Vesicles with Antifungal Activity

Palma

Ambrosone

Leone

et al. 2020

Plants

View full text Add to dashboard Cite

Extracellular Vesicles (EVs) play pivotal roles in cell-to-cell and inter-kingdom communication. Despite their relevant biological implications, the existence and role of plant EVs released into the environment has been unexplored. Herein, we purified round-shaped small vesicles (EVs) by differential ultracentrifugation of a sampling solution containing root exudates of hydroponically grown tomato plants. Biophysical analyses, by means of dynamic light scattering, microfluidic resistive pulse sensing and scanning electron microscopy, showed that the size of root-released EVs range in the nanometric scale (50–100 nm). Shot-gun proteomics of tomato EVs identified 179 unique proteins, several of which are known to be involved in plant-microbe interactions. In addition, the application of root-released EVs induced a significant inhibition of spore germination and of germination tube development of the plant pathogens Fusarium oxysporum, Botrytis cinerea and Alternaria alternata. Interestingly, these EVs contain several proteins involved in plant defense, suggesting that they could be new components of the plant innate immune system.

show abstract

Effect of anti-fungal compound phytosphingosine in wheat root exudates on the rhizosphere soil microbial community of watermelon

Cited by 42 publications

References 71 publications

The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens

The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens

Identification of Compounds with Potential Therapeutic Uses from Sweet Pepper (Capsicum annuum L.) Fruits and Their Modulation by Nitric Oxide (NO)

Plant Roots Release Small Extracellular Vesicles with Antifungal Activity

Contact Info

Product

Resources

About