Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze–thawing process

Bucak, Mustafa Numan; Ateşşahín, Ahmet; Yüce, Abdurrauf

doi:10.1016/j.smallrumres.2007.09.002

Cited by 215 publications

(144 citation statements)

References 41 publications

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“…Also, BME did not benefit ram sperm motility 2 h after thawing, contradicting findings reported for bull sperm . A potential cryoprotectant effect of cysteine at the same tested concentration, as reported elsewhere (Bucak et al, 2008), was not confirmed by our results. Future studies may test distinct periods for inclusion of antioxidants in freezing extenders.…”

Section: Discussioncontrasting

confidence: 64%

“…In Experiment 1, BME and cysteine were added to the extender before freezing, in opposition to studies with frozen sperm of bulls (Bilodeau et al, 2001), boars (Yamaguchi andFunahashi, 2012) and rams (Salem et al, 1988;Bucak et al, 2008), in which antioxidants were added to extenders after thawing. The inclusion of antioxidants prior to freezing was aimed to minimize the production of superoxide radicals that commonly occur during all steps of sperm cryopreservation (Chatterjee and Gagnon, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In such form, cysteine would serve as a substrate for GSH synthesis (Matos et al, 1997;Bai et al, 2008). Although supplementation of extenders with GSH itself and other antioxidants can protect spermatozoa against lipid peroxidation, their benefits for postthawing viability are controversial not only for ram sperm (Bucak et al, 2008;Câmara et al, 2011), but also for the sperm of bulls ) and bucks (Sinha et al, 1996). Cryotolerance of boar sperm can be improved with supplementation of freezing extenders with GSH, although such effect may be influenced by the individual freezing ability of specific ejaculates (Yeste et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the inclusion of substances with antioxidant properties in freezing extenders may prevent cryoinjuries in spermatozoa's membranes and organelles that may impair their post-thawing viability (Chatterjee and Gagnon, 2001). Many antioxidant additives have been tested in protocols for freezing ram sperm (Bucak et al, 2008;Maia et al, 2010), but due to their inconsistent results, further research in this field is justified.…”

Section: Introductionmentioning

confidence: 99%

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé

Cardoso²,

Silva³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos ABSTRACTThe effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated.Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé

Cardoso²,

Silva³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…Several antioxidants such as glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) act as a defense mechanism against the LPO and play a major role in maintaining sperm motility and viability (Bucak et al, 2008). One of the substances that is widely distributed in other types of cells is glutathione (GSH), where one of its functions is to protect cells against the destructive effect of reactive oxygen species (ROS, Meister, 1983).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Shamiah¹,

El-Karim

Eshera³

2017

Journal of Sustainable Agricultural Sciences

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IntroductionThe increasing use of artificial insemination (AI) in poultry industry emphasizes the need for good quality sperm distribution. A proper evaluation of semen prior to AI or storage is very important to avoid losses in fertility. Because semen evaluation is extremely important for semen storage and AI programs, a rapid, economical, objective and fertility predictive method of semen evaluation would be beneficial for the poultry industry protocols. Determination of antioxidant enzymes concentration in semen has been practiced for a long time (Peters, 2008).Lipids are a basic component of semen, contributing to the membrane structure of spermatozoa, the metabolism of the sperm cells, and their ability to capacitate and fertilize the female gamete. In birds, the lipid composition of spermatozoa has an influence on fertility (Ansah and Buckland, 1982). El-Saadany (2002) reported that increasing storage time of diluted semen from local chicken breed for 0, 24, 48 and 72 hr decreased significantly (P<0.01) the fertility rate (FR) by 5.12, 9.55 and 12.53%, respectively. Shamiah et al. (2017) reported that GSH supplementation in semen extender significantly (P<0.05) increased FR. Semen supplemented with 0.2 mM GSH (74.5%) showed higher FR than those supplemented with 0.6 mM GSH or free semen (59.9 and 59.2%, respectively).Sperm cells have a high content of unsaturated T HIS STUDY was carried out to evaluate the effect of glutathione (GSH) supplementation at levels of 0.0 (control), 0.2, 0.4 and 0.6 mM to diluted semen stored for 0, 24, 48 and 72 hr at 5°C, on antioxidant enzymes activities in seminal plasma, semen characteristics and fertility rate (FR) of cocks Inshas strain. Supplementation of 0.6 mM GSH to the diluted cocks semen significantly increased (P<0.05) the concentration of alkaline phosphatase (ALP) (40.2 u/ml) compared to other treatments (33.0, 27.8 and 28.3 u/ml). Superoxide dismutase (SOD) concentration in seminal plasma was not significantly affected (P<0.05) by different levels of GSH, storage period or interaction between levels of GSH and storage period. Glutathione peroxidase (GSH-Px) concentration in seminal plasma was not affected by different levels of GSH (P<0.05). Total antioxidant capacity (TACO) in seminal plasma was significantly affected (P<0.05) by different levels of GSH, storage period or interaction between them. There was a positive correlation (r=0.089 and r = 0.168) between SOD concentration and (GSH-Px and TAOC) concentration, but negative correlation (r=-0.119) with ALP concentration. Positive insignificant correlation between (ALP, SOD and TACO) concentration in seminal plasma with the progressive motility, live sperm, curled tail and fertility, while positive significant correlation between GSH-Px concentration with progressive motility, curled tail and fertility. It could be concluded that the addition of GSH to semen extender of cockerels especially at levels 0.2 mM improved the antioxidant enzymes activities, which play a vital role in lowering the deleterious effe...

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Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation

Salimi

Eslami

Farrokhi‐Ardabili

2023

Veterinary Medicine & Sci

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Background: Due to lower antioxidant capacity and higher amounts of polyunsaturated fatty acids, ram spermatozoa are very susceptible during cooling process. Objectives:The objective was to examine the effect of the trans-ferulic acid (t-FA) on the ram semen during liquid preservation.Methods: Semen samples were collected from the Qezel rams, pooled, and extended with the Tris-based diluent. Pooled samples enriched with different amounts of the t-FA (0, 2.5, 5, 10, and 25 mM) and preserved at 4 • C for 72 h. Spermatozoa's kinematics, membrane functionality, and viability were assessed by CASA system, hypoosmotic swelling test, and eosin-nigrosin staining, respectively. Moreover, biochemical parameters were measured at 0, 24, 48, and 72 h.Results: Results showed that 5 and 10 mM t-FA improved forward progressive motility (FPM) and curvilinear velocity compared to the other groups at 72 h (p < 0.05).Samples treated with 25 mM t-FA showed the lowest total motility, FPM, and viability at 24, 48, and 72 h of storage (p < 0.05). Higher total antioxidant activity levels were observed in the 10 mM t-FA-treated group compared to the negative control at 72 h (p < 0.05). Treatment with 25 mM t-FA increased malondialdehyde amounts and decreased superoxide dismutase activity compared to other groups at the final time assessment (p < 0.05). Nitrate-nitrite and lipid hydroperoxides values were not affected by treatment. Conclusions:The current study indicates the positive and negative influences of different concentrations of t-FA on the ram semen upon cold storage.

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Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze–thawing process

Cited by 215 publications

References 41 publications

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation

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