Effect of antibacterial plant flavanones on the intracellular calcium compartment involved in the first cleavage of sea urchin eggs

Biyiti, L.; Pesando, D.; Puiseux‐Dao, S.; Girard, Jean‐Pierre; Payan, P.

doi:10.1016/0041-0101(90)90063-d

Cited by 38 publications

(24 citation statements)

References 22 publications

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“…Sperm was collected dry and kept at 4°C. Shortly before use, it was diluted 1:50 in NSW, and 20 μl of this suspension were added per milliliter of egg suspension [20]. Experiments were carried out at room temperature with a 4% (v/v) egg suspension containing 20,000 eggs/ml in NSW stirred with a three‐blade propeller.…”

Section: Methodsmentioning

confidence: 99%

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Toxicity of tri‐n‐butyltin to sea urchin eggs and larvae: Relation to bioaccumulation at the nanomolar level

Girard¹,

Szpunar

Pedrotti

et al. 2000

Enviro Toxic and Chemistry

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Abstract-Nanomolar concentrations of tri-n-butyltin (TBT) from 5 ϫ 10 Ϫ10 M to 5 ϫ 10 Ϫ9 M, were assayed on sea urchin (Paracentrotus lividus) egg cleavage and on larval development. Preincubation enhanced TBT toxicity to first cleavage DNA and protein syntheses but not to intracellular calcium sequestration. Exposure to nanomolar TBT affected the larval development up to the ninth day by reducing arm length and increasing the diameter of the rudiment. Chromatographic analysis of TBT in eggs shows a dose-dependent biomagnification with a half-time of 5 min, which is much shorter than the length of preincubation necessary to provoke cytotoxicity at the same concentration (5 ϫ 10 Ϫ9 or 5 ϫ 10 Ϫ10 M). Our data suggest that nanomolar concentrations of TBT similar to those encountered in polluted waters could directly affect sea urchin egg development after fertilization and the larval cycle, these effects being independent of bioaccumulation.

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Section: Methodsmentioning

confidence: 99%

“…The rate of division was determined as described by Biyiti et al [20]. At time intervals after fertilization, the number of cleaving embryos (treated or controls) was determined under the microscope and the results were expressed as the percentages of treated eggs cleaving relative to cleaving control eggs.…”

Section: Methodsmentioning

confidence: 99%

Toxicity of tri‐n‐butyltin to sea urchin eggs and larvae: Relation to bioaccumulation at the nanomolar level

Girard¹,

Szpunar

Pedrotti

et al. 2000

Enviro Toxic and Chemistry

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“…1), respectively, together with minor amounts of further homologues and dibrominated pigments, for which we reserve the names alterochromide B/BЈ, etc. The alterochromides possessed cytotoxic effects on developing eggs of the sea urchin Strongylocentrotus intermedius with an MIC value of 40 mg/ml [2].…”

Section: Introductionmentioning

confidence: 99%

Bromoalterochromides A and A′, Unprecedented Chromopeptides from a Marine Pseudoalteromonas maricaloris Strain KMM 636T†

et al. 2007

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The marine strain Pseudoalteromonas maricaloris KMM 636 T was found to produce an inseparable mixture of two brominated yellow main pigments, bromoalterochromide A and AЈ, in a ratio of 3 : 1. Both pigments are Thr-Val-Asn-Asn-X pentapeptide lactones, where the amino group of Thr is acylated with 9-(3-bromo-4-hydroxyphenyl)-nona-2,4,6,8-tetraenoic acid, and X is aIle and Leu, respectively. They possess cytotoxic effects on developing eggs of the sea urchin Strongylocentrotus intermedius, but no antibiotic activity.

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“…Sperm was collected dry and kept at 4°C. Shortly before use, it was diluted 1:50 in seawater, and 20 μl of this suspension were added per milliliter of egg suspension [18]. Experiments were carried out at room temperature with a 4% (v/v) egg suspension containing 20,000 eggs/ml in seawater stirred with a three‐blade propeller.…”

Section: Methodsmentioning

confidence: 99%

Structure–activity relationship for bromoindole carbaldehydes: Effects on the sea urchin embryo cell cycle

Moubax

Bontemps-Subielos

Banaigs

et al. 2001

Enviro Toxic and Chemistry

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Natural derivatives of indole-3-carbaldehyde were isolated from the tropical marine ascidian Stomoza murravi. A series of 13 derivatives, three natural and 10 synthetic (brominated and N-methylated), were examined for their effects on cell division of sea urchin eggs. These derivatives were shown to inhibit the first mitotic cycle in a concentration-dependent manner. By comparing the IC50 values with the structure of the various molecules, we were able to determine that bromination increased the cytotoxicity of the compound with a maximum occurring when bromine was added to carbon number 2, while addition of N-methylation was shown to markedly reduce the cytotoxicity of these same compounds brominated at carbon 2 only. Biological activity of this family of compounds has been characterized, via detailed study of addition of the most active derivative, 2,5,6-tribromoindole-3-carbaldehyde, on macromolecule synthesis and cytoskeleton reorganization during the first mitotic cycle of fertilized sea urchin eggs. Fluorescence localization of chromatin and microtubules revealed that 2,5,6-tribromoindole-3-carbaldehyde allowed pronuclei migration and fusion but prevented the condensation of chromatin, nuclear envelope breakdown, and bipolar mitotic spindle assembly, inducing an arrest of sea urchin embryogenesis at the beginning of mitosis. It is postulated here that this phenotype is likely to be due to a strong inhibition of DNA replication and protein synthesis.

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Effect of antibacterial plant flavanones on the intracellular calcium compartment involved in the first cleavage of sea urchin eggs

Cited by 38 publications

References 22 publications

Toxicity of tri‐n‐butyltin to sea urchin eggs and larvae: Relation to bioaccumulation at the nanomolar level

Toxicity of tri‐n‐butyltin to sea urchin eggs and larvae: Relation to bioaccumulation at the nanomolar level

Bromoalterochromides A and A′, Unprecedented Chromopeptides from a Marine Pseudoalteromonas maricaloris Strain KMM 636T†

Structure–activity relationship for bromoindole carbaldehydes: Effects on the sea urchin embryo cell cycle

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