Effect of antibiotic co-administration on young and mature biofilms of cystic fibrosis clinical isolates: the importance of the biofilm model

Tré-Hardy, Marie; Macé, Camille; Manssouri, Naïma El; Vanderbist, Francis; Traore, Hamidou; Devleeschouwer, Michel

doi:10.1016/j.ijantimicag.2008.07.012

Cited by 52 publications

(41 citation statements)

References 33 publications

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“…While this study shows that overcoming the rheological barrier using co-administration of DNase contributed to potentiating ciprofloxacin, it remains to be seen whether the co-administration of ciprofloxacin and DNase would be effective in killing the highly-resistant bacteria at the later stage. For overcoming the bacterial resistance to the antibiotics, it is conceivable to co-administer multiple antibiotics (61,62) along with DNase.…”

Section: Discussionmentioning

confidence: 99%

Inhalable Antibiotic Delivery Using a Dry Powder Co-delivering Recombinant Deoxyribonuclease and Ciprofloxacin for Treatment of Cystic Fibrosis

Yang

Tsifansky

et al. 2009

Pharm Res

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Section: Discussionmentioning

confidence: 99%

Inhalable Antibiotic Delivery Using a Dry Powder Co-delivering Recombinant Deoxyribonuclease and Ciprofloxacin for Treatment of Cystic Fibrosis

Yang

Tsifansky

et al. 2009

Pharm Res

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“…The medium was changed daily from day 1 to day 12 in order to promote biofilm formation and maturation. We have shown previously that such a mature biofilm (12 days old) is a more realistic in vitro model than 24-hour-old biofilm (35).…”

Section: Methodsmentioning

confidence: 99%

“…This model simulates the in vivo growth conditions of P. aeruginosa in the upper respiratory airways of patients with CF better than planktonic and immature biofilm models. Using this model, we have previously demonstrated that the combination tobramycin-clarithromycin might be a good antibiotic association to treat P. aeruginosa in patients with CF (35).…”

mentioning

confidence: 99%

Efficacy of the Combination of Tobramycin and a Macrolide in an I n V itro Pseudomonas aeruginosa Mature Biofilm Model

Tré-Hardy

Nagant

Manssouri

et al. 2010

Antimicrob Agents Chemother

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Respiratory disease is the main cause of morbidity and mortality in patients with cystic fibrosis (CF). In particular, patients suffer from chronic infection due to biofilm formation by opportunistic Pseudomonas aeruginosa (32). Therefore, there is an urgent need to develop alternative ways to treat biofilm-associated clinical infections. The aim of this study was to compare the antimicrobial effects in vitro of the combinations tobramycin-clarithromycin and tobramycin-azithromycin against five P. aeruginosa biofilms and to establish the most effective combination. We performed a kinetic study over a period of 28 days of a twice-daily coadministration of the combinations tobramycin-clarithromycin and tobramycin-azithromycin on 12-day-old, mature P. aeruginosa biofilms formed on microplate pegs for 4 clinical isolates and one laboratory strain (PAO1) to simulate the treatment of CF patients with tobramycin inhalation solution (TOBI) through aerosolization. A synergy between tobramycin and clarithromycin was recorded for 3/5 biofilms, with a bacterial decrease of more than 5 log. Conversely, we found an antagonistic activity when 4 g/ml tobramycin was administered with azithromycin at 2 g/ml for P. aeruginosa PAO1 and with azithromycin at 2, 20, 50, 100, and 200 g/ml for P. aeruginosa PYO1. Treatment with tobramycin at 4 g/ml combined with clarithromycin at 200 g/ml eradicated all five biofilms, while tobramycin-azithromycin at the same concentrations eradicated only three biofilms. Results of this study suggest that local administration of tobramycin and clarithromycin into the respiratory tract represents a better strategy than the combination tobramycin-azithromycin for the treatment of P. aeruginosa-associated pulmonary infections.

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“…In addition, DTB and naringenin drastically increased the sensitivity of P. aeruginosa to tobramycin, confirming that biofilm formation is impaired by these treatments. The preliminary structural characterization of the responsible compounds could have medical applications as biofilm formation/structure greatly contributes to chronic infection by increasing antimicrobial resistance properties of pathogenic bacteria (Hentzer & Givskov, 2003;Ito et al, 2009;Landry et al, 2006;Tré-Hardy et al, 2009). Naringenin and the responsible compounds in DTB are therefore important lead compounds for the development of new therapeutic compounds against biofilm-encapsulated pathogens, acting by disrupting biofilm structure and so increasing pathogen exposure to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Endemic Malagasy Dalbergia species inhibit quorum sensing in Pseudomonas aeruginosa PAO1

et al. 2013

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Various species of the plant genus Dalbergia are traditionally used as medicine for sundry ailments and some of them have been shown recently to quench the virulence of Gram-positive and Gram-negative bacteria. Cell-to-cell communication mechanisms, quorum sensing (QS) in particular, are key regulators of virulence in many pathogenic bacteria. Screening n-hexane extracts of leaves, roots and bark of endemic Malagasy Dalbergia species for their capacity to antagonize QS mechanisms in Pseudomonas aeruginosa PAO1 showed that many reduced the expression of the QS-regulated genes lasB and rhlA. However, only the extract of Dalbergia trichocarpa bark (DTB) showed a significant reduction of QS gene expression without any effect on the aceA gene encoding a QS-independent isocitrate lyase. Further characterization of DTB impact on QS revealed that the QS systems las and rhl are inhibited and that swarming, twitching, biofilm formation and the production of pyocyanin, elastase and proteases are also hampered in the presence of the DTB extract. Importantly, compared with the known QS inhibitor naringenin, the DTB extract showed a stronger negative effect on twitching, biofilm formation and tobramycin resistance. Preliminary structural characterization of these potent biofilm disrupters suggests that they belong to the phytosterols. The strong inhibition of motility and biofilm formation suggests that the DTB extract contains agents disrupting biofilm architecture, which is an important observation in the context of the design of new drugs targeting biofilm-encapsulated pathogens.

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Effect of antibiotic co-administration on young and mature biofilms of cystic fibrosis clinical isolates: the importance of the biofilm model

Cited by 52 publications

References 33 publications

Inhalable Antibiotic Delivery Using a Dry Powder Co-delivering Recombinant Deoxyribonuclease and Ciprofloxacin for Treatment of Cystic Fibrosis

Inhalable Antibiotic Delivery Using a Dry Powder Co-delivering Recombinant Deoxyribonuclease and Ciprofloxacin for Treatment of Cystic Fibrosis

Efficacy of the Combination of Tobramycin and a Macrolide in an I n V itro Pseudomonas aeruginosa Mature Biofilm Model

Endemic Malagasy Dalbergia species inhibit quorum sensing in Pseudomonas aeruginosa PAO1

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