Effect of antibiotics on polymorphonuclear leukocyte functions and myeloperoxidase activity, glutathione and malondialdehyde levels in allergic asthma

Rayaman, Pervin; Rayaman, Erkan; Çevikbaş, Adile; Demirtunç, Refik; Şehirli, Ahmet Özer; Alagöz, Seyda Gül; Gürer, Ümran Soyoğul

doi:10.33073/pjm-2015-010

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…In the current study, the balance between oxidative and anti oxidative systems was assessed by measuring the serum levels of GSH and MDA. Whereas, GSH is a vital antioxidant that belongs to the non-enzymatic antioxidant defense system and scavenges free radicals directly, while MDA is an indicator of lipid peroxidation that is formed by free radicals during tissue damage, so it is used to measure oxidative stress (15).There was significant decrease in GSH and significant increase in MDA in UC group as compared to the control, and the reverse revealed in RJ group consistent with previous studies [1,3,16] .…”

Section: Discussionsupporting

confidence: 86%

Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats : A histological study

Ismail

Aboulkhair

2018

Egyptian Journal of Histology

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Introduction: Ulcerative colitis (UC) is a chronic inflammatory condition characterized by acute episodes of colonic inflammation. Its pathogenesis is associated with decreased antioxidant capability. Royal Jelly (RJ) is usually used as a complementary therapy in various diseases because of its antioxidant, anti-inflammatory and immunomodulatory effects. Aim of the work:To evaluate the protective effect of RJ against acetic acid-induced UC in adult male albino rats. Materials and Methods: Thirty rats were divided equally into 3 groups. Group I was the control group. Group II included rats subjected to intracolonic acetic acid (AA) for induction of UC. Group III 10 rats treated with RJ (250 mg/kg/day orally) for 7 days, thereafter subjected to AA. RJ was administered for another 14 days. Rats were sacrificed after 21 days. Serum glutathione (GSH) and malondialdehyde (MDA) were assessed. Colonic sections were subjected to H&E, Periodic Acid Schiff (PAS), toluidine blue, Mallory's trichrome stains and cyclooxygenase-2 (COX-2) immunohistochemical stain. Results: Group II showed significant decrease in GSH, significant increase in MDA and marked histological alterations in colon. There was a significant decrease in the number of goblet cells, but significant increases in the number of mast cells, area% of collagen fibers and COX-2 immunoexpression compared to the control. In group III, GSH was significantly increased and MDA was significantly decreased compared to group II. The colon showed minimal changes, significant increase in the number of goblet cells, significant reduction in the number of mast cells, collagen deposition and COX-2 immunoreactivity compared to group II. When compared to the control, there was no significant difference regards the serological, histological and morphometric results, except for the number of goblet cells that revealed significant decrease. Conclusion: RJ proven to protect against acetic acid-induced UC in albino rats.

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Section: Discussionsupporting

confidence: 86%

Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats : A histological study

Ismail

Aboulkhair

2018

Egyptian Journal of Histology

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“…Yu et al found that hydrogen-rich saline improved the serum MDA and SOD levels in an allergic rhinitis animal model [ 23 ] involved with suppression of eotaxin, further confirming that molecular hydrogen had protective effects on allergic disorders. MPO, which is a haemoprotein secreted during activation of neutrophils [ 24 ], was considered to stimulate the production of MDA and participate in neutrophilic airway inflammation in asthma [ 25 ]. The simultaneous decrease of MDA and MPO may suggest the interaction between these two substances.…”

Section: Discussionmentioning

confidence: 99%

Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice

Zhang

Deng

Zhang

et al. 2018

asthma res and pract

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BackgroundAsthma is a worldwide common chronic airway disease that cannot be cured and results in the huge burden in public health. Oxidative stress was considered an important mechanism in the pathogenesis of asthma. Hydrogen gas been demonstrated to function as a novel antioxidant and exert therapeutic antioxidant activity in a number of diseases and the function of this nontoxic gas in asthma was unclear. The purpose of the study aims to examine the effect of inhalation hydrogen gas on the pathophysiology of a mouse model of asthma.MethodsA murine model of ovalbumin (OVA)-induced allergic airway inflammation was used in this study. Briefly, Mice were sensitized to ovalbumin and received inhalation of 67% high concentration of hydrogen gas for 60 min once a day for 7 consecutive days after OVA or PBS challenge respectively. Lung function was assessed in the apparatus with 4 channels of biological signal system. Morphology and goblet cell hyperplasia were stained by H/E and Periodic acid-Schiff staining. Cytologic classification in the bronchial alveolar lavage fluid (BALF) was analyzed by Wright Giemsa staining. Serum, BALF and lung tissue were collected for biochemical assay. One-way analysis of variance (ANOVA) was used to determine statistical significance between groups. Multiple comparisons were made by Bonferroni’s Multiple Comparison Test by using GraphPad Prism 5 software.ResultsInhalation of hydrogen gas abrogated ovalbumin-induced the increase in lung resistance. Concomitantly, the asthmatic mice showed severe inflammatory infiltration and goblet cell hyperplasia which were reversed by hydrogen gas inhalation. Hydrogen gas inhalation reduced significantly the number of total cells, eosinophils and lymphocytes in BALF. Increased level of IL-4, IL-13, TNF-α and CXCL15 in the BALF and IL-4 in the serum were decreased significantly after inhalation. Hydrogen gas inhalation markedly upregulated the activity of decreased superoxide dismutase and significantly attenuated the increased level of malondialdehyde and myeloperoxidase.ConclusionsHydrogen gas inhalation improves lung function and protects established airway inflammation in the allergic asthmatic mice model which may be associated with the inhibition of oxidative stress process. This study provides a potential alternative therapeutic opportunity for the clinical management of asthma.

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“…& Lipid peroxidation was detected by measuring malondialdehyde (MDA) (Rayaman et al 2015). It was measured colorimetrically by a commercially available kit (Biodiagnostic, Cairo, Egypt) according to Lapenna et al (2001).…”

Section: Lipid Peroxidation Estimation and Oxidative Enzyme Assaymentioning

confidence: 99%

Impact of e-cigarettes on colonic mucosa and the role of recovery: involvement of oxidative and inflammatory pathway

Mohammed

El-Din

Farag

2021

Environ Sci Pollut Res

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Electronic cigarettes (e-cigarettes) (EC) are often advertised as a safer alternative to conventional cigarettes. Its widespread use has led to increased interest in its adverse health effects, thanks to few restrictions and a lack of regulatory guidelines. The study aimed to evaluate the influence of exposure to e-cigarette aerosol inhalation in rat colon model and conduct a follow-up after cessation of exposure. The experiment included 30 male adult Albino rats. The animals were divided into three groups: group I (control), non-exposed animals; group II (exposed), was exposed to electronic cigarette liquid vapor for four consecutive weeks; and group III (recovery), was followed up for another 4 weeks after exposure to an e-cigarette as exposed group and for the same duration. In the exposed group, malondialdehyde (MDA) and total nitric oxide (NO) increased significantly in colonic tissue, while superoxide dismutase (SOD) decreased. On histological examination, colonic mucosa showed distortion and loss of its epithelial lining with heavy inflammatory cell infiltration. Also, there was a significant decrease in periodic acid-Schiff-positive goblet cells and area percent of proliferating cell nuclear antigen expression. Tumor necrosis factor-alpha (TNFα) expression significantly increased in colonic mucosa. After 4 weeks of EC cessation, the colonic mucosal histological structure showed recovery with downregulated TNFα immunoexpression and restored oxidant/antioxidant balance. In conclusion, the usage of electronic cigarettes resulted in marked pathological alterations in the colonic mucosa, which could be attributed to oxidative and inflammatory stresses. In contrast, the cessation of exposure led to recovery.

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Effect of antibiotics on polymorphonuclear leukocyte functions and myeloperoxidase activity, glutathione and malondialdehyde levels in allergic asthma

Cited by 6 publications

References 13 publications

Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats : A histological study

Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats : A histological study

Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice

Impact of e-cigarettes on colonic mucosa and the role of recovery: involvement of oxidative and inflammatory pathway

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