Effect of Antibiotics on the Symptoms of Stunting Disease of <i>Magnolia liliiflora</i> Plants

Kamińska, M.; Śliwa, H.

doi:10.1046/j.1439-0434.2003.00682.x

Cited by 13 publications

(3 citation statements)

References 16 publications

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“…1968; Freitag and Smith 1969; Andersen et al. 2001; Kamińska and Śliwa 2003; Wongkaew and Fletcher 2004), but symptoms in treated plants mostly reappeared after the transfer of plants to antibiotic‐free medium. Bradel et al.…”

Section: Introductionmentioning

confidence: 99%

Auxin-treatment induces recovery of phytoplasma-infected periwinkle

Perica

2008

Journal of Applied Microbiology

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Aims: To test the effect of auxin‐treatment on plant pathogenic phytoplasmas and phytoplasma‐infected host. Methods and Results: In vitro grown periwinkle shoots infected with different ‘Candidatus Phytoplasma’ species were treated with indole‐3‐acetic acid (IAA) or indole‐3‐butyric acid (IBA). Both auxins induced recovery of phytoplasma‐infected periwinkle shoots, but IBA was more effective. The time period and concentration of the auxin needed to induce recovery was dependent on the ‘Candidatus Phytoplasma’ species and the type of auxin. Two ‘Candidatus Phytoplasma’ species, ‘Ca. P. pruni’ (strain KVI, clover phyllody from Italy) and ‘Ca. P. asteris’ (strain HYDB, hydrangea phyllody), were susceptible to auxin‐treatment and undetected by nested PCR or detected only in the second nested PCR in the host tissue. ‘Ca. P. solani’ (strain SA‐I, grapevine yellows) persisted in the host tissue despite the obvious recovery of the host plant and was always detected in the direct PCR. Conclusions: Both auxins induced recovery of phytoplasma‐infected plants and affected tested ‘Candidatus Phytoplasma’ species in the same manner, implying that the mechanism involved in phytoplasma elimination/survival is common to both, IAA and IBA. Significance and Impact of the Study: The results imply that in the case of some ‘Candidatus Phytoplasma’ species, IBA‐treatment could be used to eliminate phytoplasmas from in vitro grown Catharanthus roseus shoots.

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Section: Introductionmentioning

confidence: 99%

Auxin-treatment induces recovery of phytoplasma-infected periwinkle

Perica

2008

Journal of Applied Microbiology

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“…Till today, all the treatments against phytoplasmas have proved ineffective. Tetracyclines are known to have a bacteriostatic effect on phytoplasmas (Davis et al , 1968; Freitag & Smith, 1969; Šarić & Cvjetković, 1985; Bradel et al , 2000; Andersen et al , 2001; Kamińska & Śliwa, 2003). However, symptoms in treated plants would reappear after prolonged growth without the antibiotic, and phytoplasma presence was confirmed by PCR assays.…”

Section: Introductionmentioning

confidence: 99%

Effect of indole-3-butyric acid on phytoplasmas in infected Catharanthus roseus shoots grown in vitro

Perica

Lepeduš

Musić

2007

FEMS Microbiology Letters

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Phytoplasmas are noncultivable bacteria usually maintained in Catharanthus roseus shoots grown in vitro on MS medium with benzylaminopurine. The aim of our research was to examine the influence of indole-3-butyric acid (IBA) on C. roseus shoots infected with three different phytoplasma strains. Supplement of IBA in the medium supported plant growth, photosynthesis and remission of symptoms in all phytoplasma-infected shoots, but had no effect on the presence of EY-C and SA-I phytoplasma strains in tested tissue. However, HYDB phytoplasma was undetectable in approximately half of the tested shoots grown on the medium with IBA. After 1 year of IBA treatment, HYDB-infected periwinkle shoots were retransferred to the medium supplemented with benzylaminopurine. Some of the shoots showing remission of symptoms during the IBA treatment permanently escaped the infection and remained negative when tested for phytoplasma presence. This is the first report on the differential influence of plant growth regulators on phytoplasma-infected C. roseus shoots.

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“…RFLP profiles indicated that detected phytoplasmas belonged to the 16SrI group – subgroup B, now re‐classified as the species ‘ Candidatus Phytoplasma asteris’, and to the 16SrX group – subgroup A, now re‐classified as the species ‘ Candidatus Phytoplasma mali’. In Poland, 16SrI‐B subgroup strains affect several ornamental crops including magnolia, rose, lily, tulip, freesia, and bleeding heart, while the 16SrX‐A subgroup strain has been recorded less frequently, only in magnolia and rose (Kamińska & Śliwa, 2003, 2004). This report confirms infection of dahlia by aster yellows phytoplasma belonging to group 16Sr‐B, and records for the first time, infection by phytoplasma of group 16SrX‐A.…”

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confidence: 99%

Mixed infection of dahlia plants in Poland with apple proliferation and aster yellows phytoplasmas

Kamińska¹,

Śliwa

2008

Plant Pathology

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In summer 2004, five Dahlia cultorum plants of unknown cultivar, grown in garden plots in Skierniewice, Poland, exhibited bushy growth accompanied by shoot proliferation, narrowed leaf and flower bud deficiency. Research in Italy (Marzachi et al ., 1999) had indicated that stunted growth and shoot proliferation symptoms in some dahlia plants was associated with aster yellows phytoplasma infection. To detect the possible presence of phytoplasmas in dahlias in Poland, three plants showing symptoms and two symptomless plants were assayed for the presence of phytoplasma 16S rDNA by PCR. Nucleic acids were extracted from leaves and roots using DNeasy Plant Mini Kit (Qiagen). A nested PCR was done using the universal phytoplasma primer pair P1/P7, followed by primers fA/rA or R16F2n/R16R2. To detect potential mixed infection in dahlias, the phytoplasma 16SrI or 16SrX group-specific primer pairs (R16(I)F1/R16(I)R1, fAT/rAS, fAT/rPRUS or fPD/rAT) were used for nested PCR. Phytoplasma identification was accompanied by restriction fragment length polymorphism (RFLP) analysis of R16F2n/R16R2-primed PCR product digested with Alu I, Mse I, Hha I, Hpa II, Ssp I or Rsa I endonucleases.Products were generated by nested PCRs with universal and groupspecific 16(I)F1/R16(I)R1 and fAT/rAS primer pairs exclusively from two diseased D. cultorum plants. No bands were amplified from one dahlia with disease symptoms or from healthy dahlia and Catharanthus roseus plants.

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Effect of Antibiotics on the Symptoms of Stunting Disease of Magnolia liliiflora Plants

Cited by 13 publications

References 16 publications

Auxin-treatment induces recovery of phytoplasma-infected periwinkle

Auxin-treatment induces recovery of phytoplasma-infected periwinkle

Effect of indole-3-butyric acid on phytoplasmas in infected Catharanthus roseus shoots grown in vitro

Mixed infection of dahlia plants in Poland with apple proliferation and aster yellows phytoplasmas

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