Effect of antibiotics on the development of antibody-producing cells, in vitro

Uyeki, Edwin M.

doi:10.1016/0006-2952(72)90250-x

Biochemical Pharmacology

1972

DOI: 10.1016/0006-2952(72)90250-x

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Effect of antibiotics on the development of antibody-producing cells, in vitro

Edwin M. Uyeki

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1972

1986

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Cited by 7 publications

(1 citation statement)

References 22 publications

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“…In vitro techniques for culturing antibodyprodwing cells (APC) are useful for determining the effects of drugs on the various developmental stages of the immune response (1)(2)(3). By utilizing 2,4,6-trinitrophenylsubstituted horse red blood cells (TNP-HRBC) as the immunogen, the effects of drugs on the development of antibody-producing cells against both carrier (HRBC) and hapten (TNP) can be determined.…”

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Carrier and Hapten Antibody-Producing Cells, in Vitro I. Cytokinetics

Pazdernik

Uyeki

1973

Experimental Biology and Medicine

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confidence: 99%

Carrier and Hapten Antibody-Producing Cells, in Vitro I. Cytokinetics

Pazdernik

Uyeki

1973

Experimental Biology and Medicine

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Suiciding of lymphocytic precursor cells by tritiated nucleosides, in vitro

1978

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Differences in suiciding by various tritiated nucleosides were observed between two functional assays for in vitro lymphocytic precursor cell development, the hemolysin plaque-forming cell (PFC) assay and the B lymphocytic colony-forming cell (CFC-L) assay, using BDF1 mouse spleen cells. PFC growth was markedly reduced by an early (days 0-1) pulse of tritiated deoxyadenosine ([3H]dAdo), but relatively unaffected by a pulse of tritiated thymidine ([3H]dThd) during the same interval. In contrast, CFC-L formation significantly dropped after an early (day 0) [3H]dThd pulse, as well as after pulses of [3H]dAdo and the corresponding tritiated ribosides, uridine and adenosine. This implied a cycling state in an early lymphocytic precursor cell, as opposed to the PFC insensitivity to an early [3H]dThd pulse. The response pattern of colonies and clusters to [3H]dThd supported our notion of a delayed suiciding of CFC contributing to the increase in cluster numbers.

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Role of cellular density, in vitro, in anti-tumor activity of CFA-treated and immunized cells

1976

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Incorporation of tritiated deoxythymidine (3HdT) into DNA was used to measure growth, in vitro, of P815 tumor cells admixed with spleen and peritoneal effector cells. At a high tumor cell density ((1x10(5) cells per dish), using anti-theta and anti-macrophage sera, T-cells and macrophages from the peritoneum of immunized mice could be identified as cells possessing anti-tumor activity. A nonspecific inhibition by normal effector cells, which occurred at the high tumor cell density, did not occur at a lower tumor cell density (1x10(4) cells per dish). Therefore, the effects of immunization and Freund's adjuvant treatment on the anti-tumor activity of effector cells were determined more accurately when normal cells were no longer inhibitory. Thus, experimental variables dealing with cellular density (cells/mm2 of the culture vessel surface) and effector:tumor cell ratios play an important role in the anti-proliferative capacity of effector cells.

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Effect of antibiotics on the development of antibody-producing cells, in vitro

Cited by 7 publications

References 22 publications

Carrier and Hapten Antibody-Producing Cells, in Vitro I. Cytokinetics

Carrier and Hapten Antibody-Producing Cells, in Vitro I. Cytokinetics

Suiciding of lymphocytic precursor cells by tritiated nucleosides, in vitro

Role of cellular density, in vitro, in anti-tumor activity of CFA-treated and immunized cells

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