Effect of antineoplastic drugs on the expression of Bcl-2 and Bcl-xL genes in the feline T-cell leukemia cell line

Sano, Junichi; Nagafuchi, S; Yamazaki, Junpei; Oguma, Koichi; Kano, Rui; Hasegawa, Atsuhiko

doi:10.1016/j.rvsc.2005.03.001

Cited by 19 publications

(21 citation statements)

References 15 publications

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“…Lymphoma is the most common neoplasm in cats, accounting for one‐third of all tumors in cats 15 . The antineoplastic drugs generally used for chemotherapy on feline lymphoma are reported to induce apoptosis in tumor cells 9,15 . In this study, it was confirmed to be associated with apoptotic signals in feline tumor cells.…”

supporting

confidence: 72%

“…In a previous report, we identified complementary DNA (cDNA) encoding the feline Bcl‐2 gene and examined the expression of Bcl‐2 messenger RNA in a feline tumor cell line during drug‐induced apoptosis 9,10 . The nucleotide sequences and amino acid sequences of feline Bcl‐2 cDNA exhibited a high degree of similarity with those of human and mouse Bcl‐2 , suggesting that feline Bcl‐2 might be similarly essential in cell growth and apoptosis 10 .…”

mentioning

confidence: 99%

“…Moreover, the expression level of Bcl‐2 was higher in FL‐74‐UDC‐1 and FT‐1 than in FeTJ‐1 but not in the PBMC (Figure 2), indicating high expression of Bcl‐2 in T‐cell lymphoma cells. The expression of Bcl‐2 transcripts in FT‐1 (feline T‐cell lymphoma cell line) was highly induced by doxorubicin and prednisolone treatments, 9 and might have been related to the inhibition of apoptosis. Lymphoma is the most common neoplasm in cats, accounting for one‐third of all tumors in cats 15 .…”

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confidence: 99%

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Expression of Bcl‐2 in feline lymphoma cell lines

Kano

Sato

Okamura

et al. 2008

Veterinary Clinical Pathol

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confidence: 72%

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confidence: 99%

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confidence: 99%

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Expression of Bcl‐2 in feline lymphoma cell lines

Kano

Sato

Okamura

et al. 2008

Veterinary Clinical Pathol

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“…A domestic cat KIT mRNA sequence (accession number: NM_001009837.3) was used to design KIT . In all cases, glyceraldehyde 3-phosphate dehydrogenase ( GAPDH , 5'-GGAGAAAGCTGCCAA ATATG-3' and 5'-CAGGAAATGAGCTTGACAAAGTGG-3') designed from previous study [30] was used as the internal control.…”

Section: Methodsmentioning

confidence: 99%

Characterization and <i>In Vitro</i> Culture of Putative Spermatogonial Stem Cells Derived from Feline Testicular Tissue

et al. 2013

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Spermatogonial stem cells (SSCs) function to regulate the balance of self-renewal and differentiation of male gametes. SSCs have been successfully isolated and cultured in vitro in several species, but not in feline. Therefore, in this study, we aimed to culture and characterize feline SSCs. In experiment 1, testes (n=5) from different pubertal domestic cats were cryosectioned and fluorescently immunolabeled to examine the expression of SSC (GFRα-1), differentiated spermatogonium (c-kit) and germ cell (DDX-4) markers. In experiments 2 and 3, testicular cells were digested and subsequently cultured in vitro. The resultant presumptive SSC colonies were then collected for SSC identification (experiment 2), or further cultured in vitro on feeder cells (experiment 3). Morphology, gene expression and immunofluorescence were used to identify the SSCs. Experiment 1 demonstrated that varying types of spermatogenic cells existed and expressed different germ cell/SSC markers. A rare population of putative SSCs located at the basement membrane of the seminiferous tubules was specifically identified by co-expression of GFRα-1 and DDX-4. Following enzymatic digestion, grape-like colonies formed by 13-15 days of culture. These colonies expressed GFRA1 and ZBTB16, but did not express KIT. Although we successfully isolated and cultured feline SSCs in vitro, the SSCs could only be maintained for 57 days. In conclusion, this study demonstrates, for the first time, that putative SSCs from testes of pubertal domestic cats can be isolated and cultured in vitro. These cells exhibited SSC morphology and expressed SSC-specific genes. However, long-term culture of these putative SSCs was compromised.

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“…The thermal cycler (G-Storm Thermal Cycler, Somerset, United Kingdom) was set at the condition of 15 minutes at 95 °C to activate Taq DNA polymerase, 30 cycles of 30 seconds at 94 °C for denaturing, 90 seconds at 57 °C for annealing, 30 seconds at 72 °C for extension, and 10 minutes at 72 °C for the final extension. Previously published [17] and [18] forward and reverse primers for feline LHR and FSHR and GAPDH were used as shown in Table 1 …”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%