Effect of antioxidant supplementation on leucocyte expression of reactive oxygen species in athletes

Nielsen, Hilde Grindvik; Skjønsberg, Ole Henning; Lyberg, Torstein

doi:10.1080/00365510701864602

Cited by 16 publications

(16 citation statements)

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“…Vitamin E has antioxidant and free radical scavenging activities, which suggests that this vitamin may modulate oxidative DNA damage in mammalian cells (Jacobs, Connell, Rodriguez, & Seymour, 2001). Vitamin E neutralizes the increased ROS production induced by toxic substances (Nielsen, Skionsberg, & Lyberg, 2008). Vitamin E is the primary liposoluble antioxidant which may have an important role in scavenging free oxygen radicals and in stabilizing the cell membranes thus maintaining its permeability (Navarro, Arroyo, Martin, Bello, & Villalba, 1999).…”

Section: 2mentioning

confidence: 99%

Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil

Anwar

Mohamed

2015

Journal of Radiation Research and Applied Sciences

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Wheat germ oilOxidative stress Estrogen Rats a b s t r a c tThe purpose of this study was to evaluate the effect of sodium nitrate administration on some biochemical parameters and to explore the ability of Wheat germ oil (WGO) as a natural source of antioxidants to minimize the deleterious effects of sodium nitrate.The results showed significant increase in alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and significant decrease in butyryl cholinestersea (BChE) content in hepatic tissue in nitrate group as compared to control and wheat germ oil groups through the experimental period. Furthermore, there was a significant increase in thiobarbituric reactive substances (TBARS) accompanied by significant decrease in reduced glutathione (GSH) content in rat renal tissue after 28 and 42 days of treatment with drinking water containing sodium nitrate. Significant decrease was also observed in serum estradiol (E2) in group treated with nitrate through the experimental period. In addition, microscopically examination of renal tissue showed atrophy of glomerular tuft and congestion of renal blood vessels in nitrate treated group. Administration of WGO to rats with sodium nitrate suggesting role of WGO as a natural protective antioxidant agent in hepatic and renal tissues. WGO also stimulates estrogen secretion and inhibits oxidative damage that may be attributed to the presence of biologically active components (unsaturated fatty acids, unsaponifible matters and sterols matters) as antioxidant and cyto-protective activities.It can be concluded that WGO offers a great advantage for therapeutic purpose to minimized sodium nitrate free radical induced cell damage.journal h omepage: http :/ / www .e lsev ie r. co m/ lo cate/ j rras J o u r n a l o f R a d i a t i o n R e s e a r c h a n d A p p l i e d S c i e n c e s 8 ( 2 0 1 5 ) 7 7 e8 3 http://dx.

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Section: 2mentioning

confidence: 99%

Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil

Anwar

Mohamed

2015

Journal of Radiation Research and Applied Sciences

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“…This pre-treatment of vitamins restored decreased endothelial response to stressors following a HF meal in Koreans but didn’t improve endothelial response to stressors in Caucasians. Moreover, vitamins restore impaired endothelial response to stressors by scavenging free radicals, but may not improve normal endothelial response to stressors [6,32]. Several studies showed that antioxidant vitamins improved endothelial function in people with DM, coronary artery disease, and smokers but not in healthy control groups [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies showed that antioxidant vitamins improved endothelial function in people with DM, coronary artery disease, and smokers but not in healthy control groups [33,34]. In some studies antioxidant vitamins did not change oxidative stress status in healthy athletes [32]. However, in a study of young health men and women, the response to heat, unlike the response to occlusion, was increased by administration of large doses of vitamins in young, fit students at similar dosages to the present investigation and also for 2 weeks [11].…”

Section: Discussionmentioning

confidence: 99%

Protective effect of anti-oxidants on endothelial function in young Korean-Asians compared to Caucasians

et al. 2012

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SummaryBackgroundPrevious studies show that Asians have an impaired blood flow response (BFR) to occlusion after a single high fat (HF) meal. The mechanism is believed to be the presence and susceptibility to high free radicals in their blood. The free radical concentration after a HF meal has not been examined in Asians. Further the BFR to heat after a single HF meal in Koreans has not been measured.Material/MethodsThis study evaluated postprandial endothelial function by measuring the BFR to vascular occlusion and local heat before and after a HF meal and the interventional effects of anti-oxidant vitamins on improving endothelial function in young Korean-Asians (K) compared to Caucasians (C) with these assessments. Ten C and ten K participated in the study (mean age 25.3±3.6 years old). BFR to vascular occlusion and local heat and oxidative stress were assessed after a single low fat (LF) and HF meal at 2 hours compared to baseline. After administration of vitamins (1000 mg of vitamin C, 800 IU of vitamin E, and 300 mg of Coenzyme Q-10) for 14 days, the same measurements were made.ResultsThis study showed that the skin BFR to vascular occlusion and local heat following a HF meal significantly decreased and free radicals significantly increased at 2 hours compared to baseline in K (p<.001), but not in C. When vitamins were given, the BFR to vascular occlusion and local heat before and after HF meal were not significantly different in K and C.ConclusionsThese findings suggest that even a single HF meal can reduce endothelial response to stress through an oxidative stress mechanism but can be blocked by antioxidants, probably through scavenging free radicals in K. Since endothelial function improved even before a HF meal in K, endothelial damage from an Americanized diet may be reduced in K by antioxidants.

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“…The antioxidant vitamins C and E are commonly used as sports supplements [16]. Leukocytes, and in particular granulocytes, have an enormous capacity to produce ROS [17]. It has been suggested that leukocytes and erythrocytes in peripheral blood respond differently to supplementation, with leukocytes having a greater sensitivity than erythrocytes [18], and a reduction in oxidative stress occurs in response to supplementation with vitamins C and E [18].…”

Section: Introductionmentioning

confidence: 99%

Vitamin C, A and E supplementation decreases the expression of HSPA1A and HSPB1 genes in the leukocytes of young polish figure skaters during a 10-day training camp

Żychowska

Jastrzębski

Chruściński

et al. 2015

Journal of the International Society of Sports Nutrition

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BackgroundOverexpression of HSPA1A and HSPB1 has been shown to indicate stress and the degradation of damaged proteins. Therefore, the expression of these genes is often evaluated during exercise. Vitamin supplementation in young athletes may affect the expression of these genes, and help to maintain health and improve the effects of training.MethodsFourteen top junior female athletes (age 14–15y ± 0.3 SD, body mass 51 kg ± 5 SD, and BMI of 20.15 ± 0.9 SD, time in professional training 8.5 y ± 0.5 SD) attended a conditioning camp that included meals planned by a team dietitian. To examine the effects of vitamin supplementation on antioxidant status we supplemented the athletes with either vitamin A (16 ug/kg/day), vitamin C (8 mg/kg/day) and vitamin E (1 mg/kg/day) or an inert placebo. Blood samples were taken before and after (12 h post) the camp to assess the relative expression of HSPA1A and HSPB1 mRNA in leukocytes via quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsOverall, participants trained ~135 min daily (1345 min total). No statistically significant differences in HSPA1A and HSPB1 expression were observed between the groups before the camp. In the unsupplemented group, there was a non-statistically significant increase in HSPA1A expression (100% change) and a significant increase (37% change, p < 0.05) in HSPB1 expression over the study period. The supplemented group experienced a significant decrease in HSPA1A (40% change, p = 0.01) and HSPB1 (25% change p = 0.03) expression over the study period.ConclusionOur results indicate that supplementation with antioxidant vitamins decreases HSPA1A and HSPB1 mRNA expression in leukocytes, and thereby may reduce exercise-induced stress in young athletes, not only during training, but also in sports competitions.

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Effect of antioxidant supplementation on leucocyte expression of reactive oxygen species in athletes

Abstract: We observed no influence of 4 weeks of dietary antioxidant supplementation on oxidative stress status. Based on these findings, there is no rationale advising athletes to ingest antioxidant supplements in addition to their regular diet if that includes daily recommended doses of vitamins.

Cited by 16 publications

References 13 publications

Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil

Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil

Protective effect of anti-oxidants on endothelial function in young Korean-Asians compared to Caucasians

Vitamin C, A and E supplementation decreases the expression of HSPA1A and HSPB1 genes in the leukocytes of young polish figure skaters during a 10-day training camp

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