Effect of Antioxidant Water on the Bioactivities of Cells

Abstract

It has been reported that water at the interface of a hydrophilic thin film forms an exclusion zone, which has a higher density than ordinary water. A similar phenomenon was observed for a hydrated hydrophilic ceramic powder, and water turns into a three-dimensional cell-like structure composed of high density water and low density water. This structured water appears to have a stimulative effect on plant growth. This report outlines our study of antioxidant properties of this structured water and its effect on cell bioactivities. Culturing media which were prepared utilizing this antioxidant structured water promoted the viability of RAW 264.7 macrophage cells by up to three times. The same tendency was observed for other cells including IEC-6, C2C12, and 3T3-L1. Also, the cytokine expression of the splenocytes taken from a mouse spleen increased in the same manner. The water also appears to suppress the viability of cancer cell, MCF-7. These results strongly suggest that the structured water helps the activities of normal cells while suppressing those of malignant cells.

Exclusion zone and heterogeneous water structure at ambient temperature

“…We have reported these and other findings on the energizing effect of the hydrophilic powder in both the ‘contact’ and ‘non-contact’ seed model of germination and early sapling growth [ 13 ]. The similar effect was also observed in bioactivity of cells [ 21 ]. To evaluate the possibility that the non-contact biological effect of the infrared wave is due to the formation of EZs, the structure of frozen samples and electrical properties of water samples prepared by the two different methods, ‘contact’ and ‘non-contact’ were compared.…”

“…The well-known biological effects of the far-infrared wave may be interpreted from the point of view of assisting the formation of the structured water, EZ, in biological terrain. Undoubtedly, further research will be required to deal with this issue considering the complexity of the water structure and its biological effects [ 19 – 21 , 26 – 28 ].…”

“…Decreased redox potential means that the water may show an antioxidant property when it is mixed with oxidant materials. Actually, it was confirmed by the ultra-weak photon emission measurement that the non-contact treated water shows lower photon emission compared to control when it is mixed with a strong oxidant, 10 mM tert-Butyl hydroperoxide (TBHP) [ 21 ]. In this case, the difference between the control and the non-contact treated water became larger after 4 days of treatment.…”

“…However, there was almost no difference among the three water samples with different concentrations of powder, 1%, 3% and 10%. It seems that far lower concentration of powder, maybe in the ppm range, is enough to observe the same phenomenon [ 21 ].…”

“…The negative charge generated in the exclusion zone apparently moved to the right section of the container through the conductive aluminum foil and results in the reversed polarity. The increased availability of electrons by the formation of structured water can be confirmed by the decreased redox potential and the increased antioxidant property of water [ 21 ].…”