Effect of antioxidants in cryopreservation media on spotted halibut (Verasfer variegatus) sperm quality during cryopreservation

Zidni, Irfan; Lee, Hyo Bin; Yoon, Ji Hye; Park, Jung Yeol; Oh, Young Dae; Jang, Hyun Seok; Cho, Youn Su; Lee, Il Young; Lim, Han Kyu

doi:10.1016/j.aquaculture.2022.738351

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…In combination with gentamicin, a high concentration of MT reduced sperm motility and viability. MT at 100 µM was optimal for cryopreservation of spotted halibut sperm [14]. MT reportedly enhances the cryopreservation of testicular tissue of several mammalian species.…”

Section: Discussionmentioning

confidence: 92%

“…An antioxidant is a nucleophilic constituent that inhibits the harmful effects of an oxidative cellular environment. Antioxidants preserve the viability of sperm cells during storage by reducing, stopping, or preventing free-radical processes [14]. Antioxidants have been used to alleviate several fish health problems, such as reproduction and freezing fish spermatozoa [15].…”

Section: Introductionmentioning

confidence: 99%

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Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of >43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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“…In a study they conducted on Verasfer variegatus, Zidni et al froze sperms with different concentrations of Mito-TEMPO (0, 25, 50, 75, 100, 125, 150, 175 and 200 μM) and evaluated the post-thawing quality of sperms. When compared with control groups, antioxidant supplementation in sperm was found to be more effective in increasing post-thawing motility, maintaining cellular survival rates and preventing the increase in DNA damage that occurs in sperms during storing (7).…”

Section: The Effects Of Mito-tempo On Sperm Cryopreservationmentioning

confidence: 93%

The effects of Mito-TEMPO, a mitochondria targeted antioxidant, on frozen human sperm parameters

SAYLAN¹,

Dıramali²

2023

tjhealthsport

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Aim:The goal of this study is to find out if Mito-TEMPO, an antioxidant that targets mitochondria, can improve the quality of frozen human sperm.Material-Method: Normospermic 25 human semen samples were frozen and stored in solutions including different concentrations (0, 5, 10 and 50 µM) of Mito-TEMPO. After thawing, they were evaluated in terms of sperm motility, viability, morphology, chromatin integrity and apoptosis.Result: Sperm morphology was not found to change after cryopreservation. Viability was found to be significantly preserved in groups that were added Mito-TEMPO (p<0.01). When the motility of the sperms after they had been frozen was compared, the group that added 50 µM Mito-TEMPO had the most motile sperms. (p<0.05), even though there was no statistically significant difference between the groups that added 1 µM Mito-TEMPO and the groups that did not add Mito-TEMPO. In addition, chromatin decondensation and apoptosis rate decreased significantly in groups that were added Mito-TEMPO (p<0.01). Conclusion:Mitochondria targeted antioxidant Mito-TEMPO improves sperm quality that decreases after thawing.

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“…The fish were fed a commercial diet (Merk, Super Plus 7S, Korea) once daily during the study. After acclimatization, tricaine methanesulfonate (MS-222) was applied to anesthetize the fish and spermiation was stimulated through intramuscular injection of Ovaprim (0.3 mL kg −1 ; Syndel Laboratories, Nanaimo, BC, Canada) [ 6 , 7 ]. After 3 days of hormonal treatment, sperm samples were obtained by abdominal stripping and direct collection using 1.0-mL plastic syringes.…”

Section: Methodsmentioning

confidence: 99%

“…The amount of formazan (an orange dye) produced by dehydrogenase activity indicates the number of living cells. In this test, orange sperm are alive, whereas white sperm are dead [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

Zidni

Lee

Yoon

et al. 2022

Animals

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The roughscale sole, Clidoderma asperrimum is categorized as an endangered species. Sperm freezing is essential for preserving gametes. This study examined the CPA concentration, diluent, dilution ratio, and thawing temperature to design a sperm cryopreservation protocol for roughscale sole. The variables examined included sperm motility and kinematics, cell survival, fertilization, and DNA fragmentation. Sperm motility parameters were assessed via computer-assisted sperm analysis using a CEROS II instrument. Cell survival rate and DNA damage were assessed using the Cell Counting Kit-8 and single-cell gel electrophoresis assay, respectively. Sperm preservation was tested using several CPAs, including ethylene glycol, dimethyl sulfoxide (DMSO), glycerol, propylene glycol, and methanol. The diluents tested were 300 mM sucrose, 300 mM glucose, Stein’s solution, Ringer’s solution, and Hank’s solution. The optimal conditions for sperm cryopreservation were 10% DMSO + Stein’s solution. After thawing, sperm motility was highest with a 1:1 dilution ratio (sperm to CPA + diluent), at 69.20 ± 0.32%; thawing at 10 °C was optimal for post-thaw motility (72.03 ± 0.95%). The highest fertilization rate (40.00 ± 1.22%) was obtained using DMSO. The fresh sperm had the lowest tail DNA, followed by 10% DMSO + Stein’s solution. The developed cryopreservation methods can be used in roughscale sole hatcheries.

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Effect of antioxidants in cryopreservation media on spotted halibut (Verasfer variegatus) sperm quality during cryopreservation

Cited by 9 publications

References 52 publications

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

The effects of Mito-TEMPO, a mitochondria targeted antioxidant, on frozen human sperm parameters

Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

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