“…In the cultivation of medicinal plants, micro-fertilizers are commonly used to enhance both yield and bioactive substance content. Trace elements are essential nutrients for plant growth, and although the demand for them is relatively small, each trace element plays an important biological role ( Parshuram, Himangshu & Ranjan, 2022 ; Xiaodong et al, 2022 ). It has been reported that the application of micro-fertilizer (0.2% B, 0.3% Cu, 0.8% Fe, 0.4% Mn, 0.3% Zn) can increase the umbel number, seed yield per plant and per decare, thousand seed weight, as well as the essential oil content of anise ( Pimpinella anisum ) ( Günay & Gümüşçü, 2023 ).…”