Effect of ART1 on the proliferation and migration of mouse colon carcinoma CT26 cells in vivo

Abstract

Arginine-specific mono-ADP-ribosyltransferase 1 (ART1) is an important enzyme that catalyzes arginine-specific mono-ADP-ribosylation. There is evidence that arginine-specific mono-ADP-ribosylation may affect the proliferation of smooth muscle cells via the Rho-dependent signaling pathway. Previous studies have demonstrated that ART1 may have a role in the proliferation, invasion and apoptosis of colon carcinoma in vitro. However, the effect of ART1 on the proliferation and invasion of colon carcinoma in vivo has yet to be elucidated. In the present study, mouse colon carcinoma CT26 cells were infected with a lentivirus to produce ART1 gene silencing or overexpression, and were then subcutaneously transplanted. To observe the effect of ART1 on tumor growth or liver metastasis in vivo, a spleen transplant tumor model of CT26 cells in BALB/c mice was successfully constructed. Expression levels of focal adhesion kinase (FAK), Ras homolog gene family member A (RhoA) and the downstream factors, c-myc, c-fos and cyclooxygenase-2 (COX-2) proteins, were measured in vivo. The results demonstrated that ART1 gene silencing inhibited the growth of the spleen transplanted tumor and its ability to spread to the liver via metastasis. There was also an accompanying increase in expression of FAK, RhoA, c-myc, c-fos and COX-2, whereas CT26 cells with ART1 overexpression demonstrated the opposite effect. These results suggest a potential role for ART1 in the proliferation and invasion of CT26 cells and a possible mechanism in vivo.

Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions

“…However, the mechanism underlying how high blood glucose levels contribute to the development of CRC is currently unclear. Our previous studies demonstrated that ART1 may promote the proliferation, invasion and metastasis of CRC, and inhibit the apoptosis of CRC in vitro and in vivo (9,29). In addition, it was observed that the expression of ART1 in human CRC tissue from patients with T2DM is much higher than that in non-diabetic CRC tissue (Chen et al, unpublished data).…”

“…In our previous study, ART1 was revealed to upregulate the expression of p-AKT, c-Myc and c-Fos, in order to promote the proliferation and invasive potential of CT26 cells (9); ART1 also inhibits the apoptosis of CT26 cells via the PI3K/AKT pathway (29). A previous study demonstrated that the PI3K/AKT pathway serves a critical role in promoting aerobic glycolysis (31).…”

“…Our previous studies demonstrated that arginine-specific mono-ADP-ribosyltransferase 1 (ART1) promotes proliferation, invasion and metastasis of CRC in vitro and in vivo (9,10). Upregulation of the expression of ART1 activates the Ras homologue gene family mer A/ROCK1 pathway, which affects the expression of phosphorylated-AKT (p-AKT), c-Fos and c-Myc, and promotes the proliferation and invasion of CRC cells (9). Furthermore, ART1 upregulates hypoxia-…”

High Norepinephrine Status Induced Growth of Colorectal Cancer With Type 2 Diabetes Mellitus Benefited From ADP-Ribosyltransferase 1

“…Our previous studies [20,21,22,23] showed high expression of ART1 boosted malignant biological behavior of CRC, besides, ADP-ribosylation was proved to impact glucose metabolism in several pathway [35]. However, there is no research of effect of ART1 on colorectal cancer associated with diabetes mellitus.…”

“…ADP-ribosyltransferase-1 (ART1), an important single ADP ribose transferase, catalyzes the posttranslational modi cation of proteins by transferring a single ADP ribose group to the arginine residue of the protein, is believed to have a closely related to a variety of cellular biological behaviors [18,19]. In the early stage, we reported that ART1 expression changes altered the phosphorylation level of AKT, as well as the activity and expression of mTOR, GSK-3, c-myc, then affected the proliferation, invasion, metastasis, differentiation, angiogenesis and apoptosis of colon cancer CT26 cells [20,21,22]. AKT signaling pathway is one of the important pathways of cell survival, but also the important insulin signaling pathway.…”