Effect of artemether on cytokine profile and egg induced pathology in murine schistosomiasis mansoni

Madbouly, Neveen; Shalash, Ibraheem R.; Deeb, Somaya O. El; Amir, Azza M. El

doi:10.1016/j.jare.2014.07.003

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Our results also showed an increased egg burden of the liver in the TLR2-activated MSC group or decreased egg burden in the TLR4-activated MSC group, which appeared atrophy of the thymus or improved structure of the thymus, respectively. Many results also showed the correlation of Th1-type polarization with the reduction of liver eggs [25][26][27][28]. Our results also showed that the lower egg burden appeared in the TLR4-activated MSC group which had the highest level of IFN-γ and alleviated damage to the liver.…”

Section: Discussionsupporting

confidence: 67%

Immunopathology in schistosomiasis is regulated by TLR2,4- and IFN-γ-activated MSC through modulating Th1/Th2 responses

et al. 2020

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Background and aims: A marked egg-induced CD4 + T cell programmed inflammation and subsequent hepatic fibrosis characterize the pathogenesis of schistosomiasis. Mesenchymal stem cell (MSC) has been extensively studied for the treatment of schistosomiasis. However, the mechanism by which MSCs modulate the pathogenesis of schistosomiasis has not been clarified. Furthermore, the local inflammatory milieu may greatly influence the immunoregulatory properties of MSCs, and our early experiments demonstrated that Toll-like receptor (TLR)2/TLR4 agonist effected immune modulation of MSC. Here, we further investigated their modulation on the pathogenesis of schistosomiasis. Methods: Adult BALB/c male mice were percutaneously infected with 16 ± 2 pairs S. japonicum cercariae and received intravenously pretreated MSC at 1 week and 3 weeks post-infection, respectively. At 8 weeks post-infection, effects of MSC on liver histology were shown by hematoxylin and eosin (H&E) staining and Masson staining and quantitatively compared by the hepatic hydroxyproline content; α-smooth muscle actin (α-SMA), collagen type I(Col-1), transforming growth factor β (TGF-β), and tumor necrosis factor-α (TNF-α) gene expression in the liver were assessed by semi-quantitative polymerase chain reaction (PCR); the Th1/Th2 dominance among different groups was compared by analyzing CD4 + interferon-γ (IFN-γ)+ and CD4+interleukin-4 (IL-4)+T cells in the liver by flow cytometry and serum level of IFN-γ and IL-5 using enzyme-linked immunosorbent assay (ELISA). Effects of different kinds of MSC were further evaluated in vitro by the coculture system. Results: Results showed TLR4-and IFN-γ-activated MSC alleviated liver fibrosis in infected mice, without a significant increase of mortality, and unpretreated MSC showed no clear improvement; however, TLR2-and IFN-γactivated MSC displayed aggravated immunopathology. In accord with the pathological results, TLR4-and IFN-γactivated MSC groups showed moderate enhancement of Th1 response in vitro and clear Th1 dominance in vivo without leading to extreme inflammation, whereas TLR2-and IFN-γ-activated MSC not only induced Th1 response, but also triggered excessive inflammation as evidenced by atrophy of the thymus and higher TNF level in the coculture system.

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Section: Discussionsupporting

confidence: 67%

Immunopathology in schistosomiasis is regulated by TLR2,4- and IFN-γ-activated MSC through modulating Th1/Th2 responses

et al. 2020

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“…Artemether showed anti-parasitic properties toward many protozoan parasites such as Leishmania, Toxoplasma gondii and Trypansoma spp. (Mishina et al, 2007), and also a promising drug in control of schistosomiasis mansoni due to its reductive impact on worm burden and its role in improvement of hepatic granulomatous lesions (Madbouly et al, 2015). Production of artemisinin in genetically modified microorganisms is an attractive way to enable sufficient supply of the effective antimalarial agent (Zeng et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Exploring Artemisia annua L., artemisinin and its derivatives, from traditional Chinese wonder medicinal science

Shahrajabian

Sun

Cheng

2020

Not Bot Horti Agrobo

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Artemisia annua L. (Chinese wormwood herb, Asteraceae) synthesizes artemisinin, which is known as qinghaosu, considers as a unique sesquiterpene endoperoxide lactone. In traditional Chinese medicine, it has been used for the treatment of fevers and haemorrhoides. More researches on Artemisia annua L. and its derivatives, especially artemisinin and other metabolites will help to increase the knowledge and value of A. annua and its constituents. Phenolics from Artemisia annua consists of coumarins, flavones, flavonols, phenolic acids, and miscellaneous. Artemisinin has attracted much attention from scientists due to its potent antimalarial properties as secondary metabolites. Moreover, more attentions are focusing on the roles of artemisinin and its derivatives in treating obesity and metabolic diseases. They also have anti-bacterial, anti-inflammatory, anti-tumor, anti-protozoa, anti-helminthic, anti-fungal, anti-angiogenic and antiproliferation properties. The most important derivatives of Artemisia annua L. are arteether, artemether, artemiside, artemisinin, artemisone, artesunate, and dihydroartemisinin. Artemisinin also use against some cancers such as liver cancer, brain glioma, leukemia, nasopharyngeal cancer, gallbladder cancer, gastric cancer, cervical cancer, lung cancer, breast cancer and colon cancer. This important gift from ancient Chinese traditional medicine can guarantee health of people all around the world. Further researches should be done on the new advances and development of artemisinin and its derivatives as potential natural medicine in the global fight against so many diseases, malaria included.

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“…It was first described as anti-schistosomal agent in 1980s, against juvenile worms (5-21day-old) S. japonicum ( Liu et al, 2012 ). Artemether efficiency as a schistosomicidal and molluscicidal agent has been previously shown ( Al-Kazzaz et al, 2014 ; Madbouly et al, 2015 ).…”

Section: Introductionmentioning

confidence: 93%

Efficacy of Miltefosine and Artemether on infected Biomphalaria alexandrina snails with Schistosoma mansoni: immunological and histological studies

et al. 2020

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SummaryBiomphalaria alexandrina snails have received much attention due to their great medical importance as vectors for transmitting Schistosoma mansoni infection to humans. The main objective of the present work was to assess the efficacy of miltefosin a synthetic molluscicidal drug and artemether a natural molluscicidal drug. The correlation between immunological and histological observations from light and electron microscopy of the hemocytes of B. alexandrina post treatment with both drugs was also evaluated. LC50 and LC90 values were represented by 13.80 ppm and 24.40 ppm for miltefosine and 16.88 ppm and 27.97 ppm for artemether, respectively. The results showed that the treatment of S. mansoni-infected snails and normal snails with sublethal dose of miltefosine (LC25=8.20 ppm) and artemether (LC25=11.04 ppm) induced morphological abnormalities and a significant reduction in hemocytes count.

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Effect of artemether on cytokine profile and egg induced pathology in murine schistosomiasis mansoni

Cited by 12 publications

References 21 publications

Immunopathology in schistosomiasis is regulated by TLR2,4- and IFN-γ-activated MSC through modulating Th1/Th2 responses

Immunopathology in schistosomiasis is regulated by TLR2,4- and IFN-γ-activated MSC through modulating Th1/Th2 responses

Exploring Artemisia annua L., artemisinin and its derivatives, from traditional Chinese wonder medicinal science

Efficacy of Miltefosine and Artemether on infected Biomphalaria alexandrina snails with Schistosoma mansoni: immunological and histological studies

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