Effect of Astrocytic Energy Metabolism Depressant on ¹⁴C-Acetate Uptake in Intact Rat Brain

Abstract

Summary: Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of 14 C-acetate was measured using autoradiography. A significant reduction (more than 80%) in 14 C-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/L) infusion. This effect was transient, and 14 C-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using 14 Ciodoamphetamine, was significantly increased by the fluorocitrate infusion. The present observations indicate that 14 Cacetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.

Stimulation-Induced Increases of Astrocytic Oxidative Metabolism in Rats and Humans Investigated with 1-11C-Acetate

“…It was previously suggested that the astrocytespecific substrate acetate allows to measure metabolism in human brains in vivo (Hosoi et al, 2004;Muir et al, 1986). However, to our knowledge this is the first study using label clearance from brain after 1-11 C-acetate administration for the investigation of astrocytic oxidative metabolism in vivo.…”

Glial–Cytokine–Neuronal Interactions Underlying the Mechanisms of Persistent Pain

“…The response frequencies to a range of von Frey filament forces were determined, a stimulus-response frequency relationship established, and EF 50 values derived. After establishing the behavioral hyperalgesia at 1 d after CFA injection, fluorocitrate, a gliotoxin that is relatively selective against astroglia (Hosoi et al, 2004), was microinjected into the ventral Vi/Vc transition zone through a chronically implanted guide cannula (Fig. 8a).…”

Infusion of gliotoxins or a gap junction blocker in the prelimbic cortex increases alcohol preference in Wistar rats

“…Fluorocitric acid inhibits aconitase, a TCA cycle enzyme, enriched in astrocytes, that transforms citrate into isocitrate (Peters, 1957). Thus, FC application results in interruption of ATP production and damage to astrocytes (Paulsen, et al, 1987;Hosoi, et al, 2004;Peters, 1957). On the other hand, glutamate reuptake via glutamate transporters and the cystine-glutamate antiporter is heavily dependent on astrocytes (Anderson and Swanson, 2000).…”