Effect of AT<sub>1</sub> receptor blockade on hepatic redox status in  SHR: possible relevance for endothelial function?

Cediel, Eva; Sanz-Rosa, D.; Oubiña, M. Pilar; Heras, Natalia de las; Pacheco, Francisco R. González; Vegazo, Onofre; Jiménez, Javier; Cachofeiro, Victoria; Lahera, Vicente

doi:10.1152/ajpregu.00643.2002

Cited by 38 publications

(42 citation statements)

References 46 publications

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“…The cDNA was stored at -20°C for subsequent analysis. The primer sequences were all obtained from previous published journal articles [35-37] (Geneworks, Australia) (Table 5). Real-time PCR was performed using the iQ5 multicolour real-time PCR detection system (Bio-Rad, USA), with PCR reactions carried out using the iQ SYBR Green Supermix (Bio-Rad, USA).…”

Section: Methodsmentioning

confidence: 99%

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The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

et al. 2011

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BackgroundThis study aimed to determine if 25 days of canola oil intake in the absence of excess dietary salt or together with salt loading affects antioxidant and oxidative stress markers in the circulation. A further aim was to determine the mRNA expression of NADPH oxidase subunits and superoxide dismutase (SOD) isoforms in the aorta of stroke-prone spontaneously hypertensive (SHRSP) rats.MethodsMale SHRSP rats, were fed a defatted control diet containing 10% wt/wt soybean oil or a defatted treatment diet containing 10% wt/wt canola oil, and given tap water or water containing 1% NaCl. Blood was collected at the end of study for analysis of red blood cell (RBC) antioxidant enzymes, RBC and plasma malondialdehyde (MDA), plasma 8-isoprostane and plasma lipids. The aorta was removed and the mRNA expression of NOX2, p22phox, CuZn-SOD, Mn-SOD and EC-SOD were determined.ResultsIn the absence of salt, canola oil reduced RBC SOD and glutathione peroxidase, and increased total cholesterol and LDL cholesterol compared with soybean oil. RBC glutathione peroxidase activity was significantly lower in both the salt loaded groups compared to the soybean oil only group. In addition, RBC MDA and plasma HDL cholesterol were significantly higher in both the salt loaded groups compared to the no salt groups. Plasma MDA concentration was higher and LDL cholesterol concentration lower in the canola oil group loaded with salt compared to the canola oil group without salt. The mRNA expression of NADPH oxidase subunits and SOD isoforms were significantly reduced in the canola oil group with salt compared to canola oil group without salt.ConclusionIn conclusion, these results indicate that canola oil reduces antioxidant status and increases plasma lipids, which are risk factors for cardiovascular disease. However, canola oil in combination with salt intake increased MDA, a marker of lipid peroxidation and decreased NAPDH oxidase subunits and aortic SOD gene expression.

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Section: Methodsmentioning

confidence: 99%

“…The primers were all obtained from previous published sequences and were ordered through Geneworks (Australia): NOX2 [37], p22 phox [35], CuZn-SOD, MnSOD and Ec-SOD [36]. …”

Section: Methodsmentioning

confidence: 99%

The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

et al. 2011

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“…In previous studies, we found that 2 g but not 1 g of resting tension evidenced reduction of ACh relaxation in SHR compared with WKY. 25 When tension was stable, experiments were initiated by obtaining a reference contractile response to 75 mmol/L KCl. Endothelium-dependent relaxations were studied by evaluating relaxations to ACh (10 Ϫ10 to 10 Ϫ5 mol/L) in noradrenaline precontracted rings (1.5Ϯ0.18 g).…”

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confidence: 99%

Participation of Prostacyclin in Endothelial Dysfunction Induced by Aldosterone in Normotensive and Hypertensive Rats

et al. 2005

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Abstract-The aim of the present study was to analyze the possible involvement of vasoconstrictors prostanoids on the reduced endothelium-dependent relaxations produced by chronic administration of aldosterone in Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). For this purpose, acetylcholine (ACh) relaxations in aortic segments from both strains were analyzed in absence and presence of the cyclooxygenase-1 (COX-1) and COX-2 inhibitor indomethacin, the specific COX-2 inhibitor NS-398, the TP receptor antagonist (SQ 29 548), the thromboxane A 2 (TXA 2 ) synthase inhibitor furegrelate, and the prostacyclin (PGI 2 ) synthesis inhibitor tranylcypromine (TCP). In addition, COX-2 protein expression was studied by Western blot analysis. Release of prostaglandin E 2 (PGE 2 ) and the metabolites of PGF 2␣ , TXA 2 , and PGI 2 , 13,14-dihydro-15-keto PGF 2a , TXB 2 , and 6-keto-PGF 1␣ , respectively, were measured. Treatment with aldosterone did not modify blood pressure levels in any strain. However, aldosterone markedly reduced (PϽ0.05) ACh-induced relaxations in segments from both strains in a similar extent. Indomethacin, NS-398, SQ 29 548, and TCP enhanced (PϽ0.05) ACh relaxations in both strains treated with aldosterone. Aortic COX-2 protein expression was higher in both strains of rats treated with aldosterone. In normotensive animals, aldosterone increases the ACh-stimulated aortic production of 13,14-dihydro-15-keto PGF 2a, PGE 2 , and 6-keto-PGF 1␣ (PϽ0.05). In SHR, ACh only increased the 6-keto-PGF 1␣ production (PϽ0.05). It could be concluded that chronic treatment with aldosterone was able to produce endothelial dysfunction through COX-2 activation in normotensive and hypertensive conditions. PGI 2 seems to be the main factor accounting for endothelial dysfunction in hypertensive rats, whereas other prostanoids besides PGI 2 appear to be involved in endothelial dysfunction under normotensive conditions. Key Words: aldosterone Ⅲ endothelium Ⅲ prostacyclin Ⅲ normotension Ⅲ hypertension A ldosterone is a mineralocorticoid that participates in electrolyte balance and plays an important physiological role in the long-term regulation of Na ϩ and K ϩ in the distal tubule and collecting duct. [1][2][3][4] To date, several studies suggested that aldosterone plays a larger role than once appreciated in the regulation of vascular tone as well as in cardiovascular alterations such as endothelial dysfunction, vascular fibrosis, and inflammation, left ventricular hypertrophy, congestive heart failure, and cardiac arrhythmias. [5][6][7][8][9][10][11][12] Furthermore, aldosterone has also been shown to be involved in the pathogenesis of hypertension. [13][14][15] In general terms, endothelial dysfunction is characterized by reduced endothelium-dependent relaxations, suggesting a reduced availability of NO attributable to a reduction of NO production or enhanced NO inactivation. 16,17 In addition, an increased production of vasoconstrictor factors could be also responsible for the reduced endothelium-dependent...

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“…Some studies have shown that dietary ALA has a SBP-lowering effect, and the present findings were in accord with this 15,[22][23][24] . Endothelial dysfunction is attributed to a reduction of NO secretion associated with a reduction of endotheliumdependent relaxation and the development of vascular remodeling [25][26][27][28] . In particular, a reduction of vascular relaxation in the large arterial circulation is regarded as a major factor in the development of systolic hypertension 29) .…”

Section: Resultsmentioning

confidence: 99%

Effect of Dietary Alpha-Linolenic Acid on Vascular Reactivity in Aorta of Spontaneously Hypertensive Rats

et al. 2009

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Several studies of hypertension have shown that dietary alpha-linolenic acid (ALA) has a blood pressure-lowering effect. In the present study, we investigated the effect of dietary ALA on endotheliumdependent vascular reactivity in six-week-old spontaneously hypertensive rats (SHR). At the end of the feeding period, systolic blood pressure in the ALA group was significantly lower than in the control group. There was no significant difference in endothelium-dependent vasorelaxant response between the two diet groups. There was also no significant inter-group difference in the vascular wall thickness and media-tolumen ratio. In conclusion, the present findings suggest that the blood pressure-lowering mechanism of dietary ALA does not involve increased sensitivity to NO or a change the in vascular morphology of the aorta.

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Effect of AT₁ receptor blockade on hepatic redox status in SHR: possible relevance for endothelial function?

Cited by 38 publications

References 46 publications

The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

Participation of Prostacyclin in Endothelial Dysfunction Induced by Aldosterone in Normotensive and Hypertensive Rats

Effect of Dietary Alpha-Linolenic Acid on Vascular Reactivity in Aorta of Spontaneously Hypertensive Rats

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Effect of AT1 receptor blockade on hepatic redox status in SHR: possible relevance for endothelial function?

Cited by 38 publications

References 46 publications

The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

Participation of Prostacyclin in Endothelial Dysfunction Induced by Aldosterone in Normotensive and Hypertensive Rats

Effect of Dietary Alpha-Linolenic Acid on Vascular Reactivity in Aorta of Spontaneously Hypertensive Rats

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Effect of AT₁ receptor blockade on hepatic redox status in SHR: possible relevance for endothelial function?