Effect of ATP and molsidomine combination on contractile function of isolated adult and old rat hearts during adequate coronary perfusion, at ischemia and reperfusion

Abstract

Abstract. Pathology of the cardiovascular system occupies a major place in the structure of diseases of the elderly and old patients. Metabolic disturbances are very important in ischemic damages of myocardium in the elderly and old people. So, drugі with metabolic mechanism of action is very ppromising in the treatment of elderly patients with cardiovascular diseases. The relevance of this study is determined by the feasibility of using drugs of metabolic action, which have a beneficial effect on the metabolism of cardiomyocytes, improve blood supply to the myocardium, increase its contractile function. The effect of ATP-molsidomine combination on myocardial contractility in different age animals was stuiesy in vitro experiments. The experiments on the isolated hearts from adult and old rats have shown that combined use of ATP and molsidomine did not significantly affect the contractility of the isolated hearts of adult rats under different perfusion regimes. In old rats, the use of ATP-molsidomine combination had a positive effect on the contractile function of the myocardium under the influence of damaging factors (ischemia, reperfusion): prevented a decrease of left ventricular developing pressure and its first derivative (velocity of pressure rise and velocity of pressure decline) and accelerated its growth during reperfusion. Co-administration of ATP and molsidomine during ischemia had a positive effect on the heart rhythm and restored heart rate at the reperfusion period in adult and old rats. The results of the study indicate a positive effect of the ATP-molsidomine combination on the myocardial contractility in old rats. Combined use of ATP and molsidomine exerted a favourable influence on the heart rhythm under damaging factors both in the adult and old animals. Key words: ATP; molsidomine; isolated rat heart; myocardial contractility; ageing

Pharmacology-based toxicity assessment of molsidomine and ATP-LONG combination with singular and repetitive injections under experimental conditions

“…A preclinical study of the specific activity of molsidomine in conjunction with ATP-LONG, performed on younger and spontaneously hypertensive lab animals, revealed vasodilator, cardio-and neurotropic, and antistress effects, thereby allowing the following combination to be used in geriatrics as a possible therapeutic and prophylactic cure [6,7]. The other equally important stage in preclinical studies of pharmaceuticals, including new combinations of already known bioactive substances, is the study of their toxicity.…”

“…In Ukraine, in practice, molsidomine is most often prescribed at a dose of 2 mg two times a day, which allows reducing the frequency of side effects but calls into question the effectiveness of therapy [26]. In this case, the advantage of the combination of molsidomine with ATP-long is the ability to achieve a higher vasodilatory effect at a lower dose of molsidomine, which has been proven in animal trials [7].…”