Effect of auxin physiological analogues on rapeseed (Brassica napus) cold hardening, seed yield and quality

Abstract

The effect of the auxin physiological analogues analogues 1-[2-chloroethoxycarbonylmethyl]-4-naphthalenesulfonic acid calcium salt (TA-12) and 1-[2-dimethylaminoethoxicarbonylmethyl]naphthalene chlormethylate (TA-14) TA-14 on different winter rapeseed cultivars were studied with regard to their autumnal growth, cold hardening, accumulation of the stress-protective metabolites proline and saccharide in plant organs: apical bud and root collum, winter survival and productivity formation. The test cultivars were the very early 'Libea' medium-resistant to wintering, the medium-early 'Sunday' resistant to wintering, the medium-early 'Valesca' less than medium resistant to wintering, and the early 'Hornet' (hybrid) tolerant to stress growth conditions. During the period of cold hardening in natural field conditions, the test compounds TA-12 (2 mM) and TA-14 (4 mM), applied to different winter rapeseed cultivars at the 4th-5th leaf stage, stimulate accumulation proline and saccharides (sucrose and glucose) in the root collum and apical bud tissues, influence plants acclimation to cold, overwintering and productivity formation. Compounds TA-12 and especially TA-14 produced a stable effect on seed and crude fat yield in cvs. 'Hornet', 'Sunday' and 'Libea'. The genotypic peculiarities of a cultivar and the meteorological conditions of the plant vegetation period were the factors that mostly determined fatty acid content in seed oil.

Transcriptome Profile Analysis of Winter Rapeseed (Brassica napus L.) in Response to Freezing Stress, Reveal Potentially Connected Events to Freezing Stress

“…In Arabidopsis and Rice, a significant number of auxin-regulated genes are additionally affected by cold stress [44,49]. When auxin analogs were applied on Brassica napus , it stimulated the accumulation of freeze-protective metabolites and soluble sugars during cold hardening [50]. Therefore, we speculated that auxin might play an important role in Brassica napus response to freezing stress.…”

The Application of Auxin-like Compounds Promotes Cold Acclimation in the Oilseed Rape Plant

“…In our previous study, it was shown that after treatment with the auxin-like compounds TA-12 and TA-14, the winter resistance of the studied oilseed rape cv. ‘Valesca’ increased [ 32 ]. In the current study, we extend knowledge about the integration of auxin-like compounds into cell systems during the preparation for wintering.…”

“…An analysis of the structure and activity of these auxin-like compounds has shown that the activity of the compounds TA-12 and TA-14 is determined by the two methylene groups in their molecules, as well as a sulfo group that is attached to the naphthalene ring (TA-12) and a methyl group (CH 3 ) that is attached to N + (TA-14). Our previous study showed the effect of auxin-like compounds on the accumulation of proline and saccharides in the root collar and terminal bud in oilseed rape and, furthermore, that these compounds influence the acclimation of plants to the cold, overwintering, and productivity formation [ 32 ]. However, from a theoretical point of view, these results also raised a question regarding which metabolic processes may also be integrated into the metabolism of the cell systems during the cold acclimation period, successfully overwintering, and maintaining dormancy in plants.…”

Influence of Nostoc entophytum and Tetracystis sp. on Winter Survival of Rapeseed

“…They contribute to the development of rootage [15] and nutrient absorption, increase photosynthetic activity and improve their ability to cope with environmental stress factors [16,17]. For example, application of artificial auxin analogues, increased the content of proline and saccharose of the roots and shoot tips of rape, which led to the survival of an increased number of overwintered plants and also improved their growth indicators [18].…”